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B Dastugue

Publications and source records attributed to B Dastugue.

At least 145 records · Page 8Linked to original sources
PubMedDate not supplied

Human tears: normal protein pattern and individual protein determinations in adults.

Proteins in tears are known to be highly heterogeneous. We have tried to take into consideration some of the difficulties encountered in studying tear proteins such as tear collection, the choice of the parameters to be studied, the adequacy of techniques for small sample volumes, etc. Levels of total tear proteins were determined in 101 samples and the electrophoreses were performed. On gel slabs, four major proteins were always found to be present: lactoferrin, lysozyme and two tear specific proteins. Concentrations of some individual proteins, lactoferrin, lysozyme, IgA, IgG and albumin were determined. Comparisons of lactoferrin and lysozyme contents were found to be more reliable when based on total protein rather than on their own concentrations as such.

Adult↗
PubMedDate not supplied

[Outcome of hyperlactatemia and lipodystrophy syndromes in patients infected with human immunodeficiency virus].

HIV-positive persons requiring a highly active antiretroviral therapy containing one or more nucleosidic reverse transcriptase inhibitors associated with or without protease inhibitors are exposed to metabolic side effects among which lipodystrophy and hyperlactemia, defined by blood lactates higher than 2,25 mmol/L. Hyperlactatemia had to be differentiated from lactic acidosis of type B (without hypoxemia, lactates higher than 5 mmol/L and arterial pH lower than 7,3), a rare but potentially fatal complication by multi-visceral failure. The accused INRT induce mitochondrial toxicity by inhibition of DNA gamma polymerase and deterioration of its DNA. Our exploratory study, troop of 282 patients, identified age and stavudine like statistically associated, which has occurred of this metabolic anomaly. The patients having profited of a therapeutic change with the profit from drugs minus hyperlactatogenic presented an obvious clinical and biological improvement; whereas similar switch of therapy occurred for the lipodystrophic patients presented any clinical improvement. Nevertheless, biological parameters (blood lactates, triglycerides, total cholesterol and LDL-cholesterol) were significantly decreased after this therapeutic switch occurred on the lipodystrophic patients. In conclusion, the measurement of the following biological parameters: glycemia, lactatemia, triglycerides, total cholesterol and LDL-cholesterol at patient VIH, in a simple and rigorous pre-analytical and analytical context, appears to us justified in the monitoring of metabolic disorders in treated HIV patients by INRT and/or IP.

Acidosis, Lactic↗
PubMedDate not supplied

[Evaluation of rapid, semi-quantitative assay of C-reactive protein in whole blood, Actim CRP].

We evaluated a rapid and semi-quantitative C-Reactive Protein test on whole blood, the Actim CRP (Fumouze). Based on immuno-chromatography technology, this test ranked the blood sample in four groups: < 10 mg/L, 10-40 mg/L, 40-80 mg/L and > 80 mg/L. This evaluation finds an excellent repeatability, the absence of hook-effect for high levels of CRP and an independence from classical biological interferences: haemolysis, turbidity and bilirubin. The correlation is excellent between the rapid test and classical immuno-turbidimetric plasmatic CRP assay. This test with established analytical properties could be placed as an interesting alternative to replace the classical assays realised on analysers, and more particularly in case of reduced sample volume. The use in "patient care" context had to follow rigorous manufacturer's recommendations to respect analytical specificities identified during our validation process.

Amyloid↗