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Biomedical subjects

B Dastugue

Publications and source records attributed to B Dastugue.

At least 109 records · Page 6Linked to original sources
PubMed1981

Enzyme studies and neonatal brain damage.

The authors report a double-blind study of 57 full-term newborn infants prospectively subjected to clinical, electroencephalographical, blood and cerebrospinal fluid, and developmental examinations. Four enzymatic activities were measured in blood and CSF: aminotransferase (ASAT), creatine kinase (CK), lactate dehydrogenase (LD) and hydroxybutyrate dehydrogenase (HBD). Close relationships of enzymatic levels with psychomotor outcome are reported. In blood, ASAT and CL seemed to be the most important determinations, allowing threshold-values to be suggested. In CSF, LD and HBD were the determinations the most closely related to psychomotor events at age one. This method seems to be of theoretical as well as practical importance in evaluating neonatal brain injury.

Apgar Score↗
PubMed1980

A rapid and sensitive fluorometric assay of serum phospholipid.

A fluorometric assay of phospholipid in serum is presented. The reaction is based upon the binding of phospholipids with a fluorescent probe: 1-6-Diphenyl-1-3-5 hexatriene (DPH). The method is in very good agreement with other techniques for phospholipid estimation: phosphorus assay phospholipid extraction (Fiske and Subarrow), and enzymatic assay of phospholipid containing choline (Takayama). The correlation coefficient obtained was 0.81, when the proposed method was compared with the two other ones. Parameters influencing the reaction: DPH concentration, time and temperature of incubation are described. The normal range for human serum lies between 2.1 and 3.4 mmol/l. The sensitivity of the fluorometric assay is also sufficient for the determination of phospholipid in amniotic fluid and broncho-alveolar washings.

Amniotic Fluid↗
PubMed1979

G6PD deficiency with Gd(-)A like variant in a Chinese family from Cambodia.

A low rate value of G6PD was found in red blood cells from a Cambodian boy. Enzyme mapping was performed according to the WHO standard methods. G6PD presented all the characteristics of the A(-) variant encountered in the Negroes and behaved distinct from fast migrating enzymes described in China. No negro was in the ancestry of the mother.

Adult↗
PubMed1979

[Glycosylated hemoglobin: résults of 550 cases (author's transl)].

A selective estimation of glycosylated hemoglobin, HbA1C, exclusive of other minor components, is propably one of the best available tests. Such an automated method is proposed, which was experimented in 550 cases. It demonstrates a quite narrow gaussian distribution in controls. In contrast, a large dispersion is observed among long term diabetics, most of them with ocular complications with only a minority of results in the normal range. This is still more evident in case of juvenile diabetes.

Chromatography↗
PubMed1979

Quantitation of hemoglobin A1c: a rapid, automated precision-chromatography technique.

Hemoglobin A1c is increased in patients with diabetes mellitus and its level reflects the status of blood glucose equilibrium over a period of several weeks. The practical use of its estimation was hampered by technical difficulties in investigating large series of samples. In order to apply this examination for routine purposes we describe in this paper acceleration and full automatization of the original chromatographic method allowing quantitation of hemoglobin A1c in 45 min.

Autoanalysis↗
PubMed1979

Interaction of non-histone proteins with DNA and chromatin from Drosophila and mouse cells. Specificity, isolation and analysis of complexes.

The specificity of the binding of purified non-histone proteins to DNA has been investigated through two types of experiments. Using a nitrocellulose filter assay at a low protein/DNA ratio, the binding of mouse non-histone proteins to mouse DNA was twice as great as the binding of mouse non histone protein to Drosophila DNA. The reverse experiment using Drosophila non-histone protein confirmed the interpretation that some protein . DNA complexes were specific. Protein . DNA complexes isolated by gel filtration chromatography indicated that 20% or 10% of the non-histone protein was bound to homologous or heterologous DNA respectively. Purified non-histone proteins bound with lower efficiency (15%) than unpurified but with higher specificity to soluble chromatin than to naked DNA. This binding did not result from an exchange between chromatin non-histone proteins and purified non-histone proteins added in excess. DNA-bound and chromatin-bound proteins were analysed on polyacrylamide gels. Whereas no major qualitative differences were observed with DNA-bound proteins, some proteins bound to homologous mouse chromatin were different from those bound to heterologous Drosophila chromatin. These results suggest a possible role of DNA-bound non-histone proteins in the regulation of gene expression.

Animals↗
PubMed1979

Regulation of transcription by DNA-bound non-histone nuclear proteins.

Purified non-histone proteins from mouse mammary cells bind specifically to homologous DNA or chromatin. Complexes of non-histone protein with DNA or chromatin, isolated on agarose columns, were transcribed with both Escherichia coli RNA polymerase and RNA polymerase B from calf thymus. The fact that complexing of DNA with non-histone proteins increases transcription by E. coli RNA polymerase but not by RNA polymerase B suggests different mechanisms of transcription by these two enzymes. Similar experiments with mouse and Drosophila chromatin indicate that non-histone proteins specifically stimulate the transcription of mouse chromatin by RNA polymerase B. Non-histone proteins stimulate the transcription of mouse mammary tumor virus sequences in chromatin by RNA polymerase B but not by E. coli RNA polymerase. We conclude that those non-histone proteins bound specifically to chromatin are able to activate the transcription of specific genes by eukaryotic RNA polymerase.

Animals↗
PubMed1979

[Purification of pineal antigonadotropic factor (author's transl)].

Pineal activity on hypothalamo-hypophyso genital axis is well established. The mechanism is not simple, since progonadotropic or/an antigonadotropic activity were described. The biochemical isolation of the potent factor has been assayed. Several substances: melatonin, AVT, peptides have antigonadotropic properties. In this paper, we report many experiments concerning isolation and purification by several chromatographies of the factor (probably peptidic), that we believe to be responsible of antigonadotropic activity. Biological activity of the substance is proved by "in vitro" methods and radio immunoassays. This component is quite pure because electrophoresis on gel polyacrylamid reveals only one strip. Gel Sephadex G10 chromatography indicates a probable MW is between 500 and 1,000. This component causes an important inhibition of pituitary LH discharge (in vitro methods).

Animals↗
PubMed1979

[Foetal pulmonary maturity: estimation by the palmitic/stearic acids ratio and by the Clements' test in amniotic fluid (author's transl)].

Two parameters have been used to estimate the foetal pulmonary maturity: Clements' test or bubble test and palmitic acid/stearic acid ration. The reliability and the limits of these tests have been studied in function of several pathological pregnancies and the gestations's age. A value greater than 5 for the P/S ratio was a good pronostic at any gestational age. A value lesser than 5 should be examined according to this gestational age. A negative Clements' test has to be confirmed by an another amniocentesis, while a positive test have been followed always by a mature newborn's birth.

Amniocentesis↗
PubMed1979

[Lipids and phospholipids in the fluid of broncho-alveolar lavage. Value for the study of alveolar penetration of corticosteroids by aerosol (author's transl)].

Broncho-alveolar lavages from 13 control subjects have been studied for the following parameters : phospholipids, free fatty acids, total last parameter, through the depolarisation of Diphenylhexatriene, is independent of the recovered volume. An identical study has been performed for two bronchiolo-alveolar cancers. A treatment with hydrocortisone-containing aerosol has been associated with an increase in phospholipid amount and a drastic decrease in microviscosity. This observation suggests that the synthesis of tensio-active phospholipids is dependant of corticoids by an enzymatic induction. The effect of therapeutics given in aerosol can be monitored by the biochemical and physical modification in broncho-alveolar fluids thus obtained.

Adrenal Cortex Hormones↗
PubMed1978

Cerebrospinal fluid enzymology: creatine kinase, lactate dehydrogenase activity and isozyme pattern as a brain damage index.

Blood serum and cerebrospinal fluid from a 4.5-year-old girl suffering from convulsive episodes of toxic origin were investigated for lactate dehydrogenase (LDH) and creatine kinase (CK) activities. Elevated levels of both enzymes were found. Furthermore CK was higher in the CSF (680 I.U./1) than in blood serum (160 I.U./1). The CSF activity was demonstrated mainly as the BB form (96%) for CK and H4 (63%) was the predominant form for LDH. Identical investigations were performed 45 h later and results compared with the first set. These data provide an additional example of interest in CSF enzymatic studies as a brain damage index.

Alcoholic Intoxication↗
PubMed1978

[The determination of salivary pH by contact pH meter in individuals receiving psychotropic therapy. Study of pH on the tongue and at the orifice of Wharton's and Stenon's ducts (author's transl)].

The authors studied salivary pH at different sites in 172 patients receiving psychotropic therapy in hospital. For statistical purposes, the study was limited to individuals receiving a "moderate" dose of: --a benzodiazepine derivative --a neuroleptic and benzodiazepine --antidepressant, neuroleptic and benzodiazepine. Regardless of the type of psychotropic therapy and of the site of measurement, acidification of salivary pH which was statistically significant in comparison with values found in healthy subjects was noted. This acidification was particularly marked with regard to "lingual" pH. In addition, regardless of the site of measurement, a higher degree of acidification of salivary pH was seen in individuals receiving a combination of psychotropic agents. This acidification was particularly striking with regard to salivary pH in patients given a combination of antidepressant, neuroleptic and a benzodiazepine derivative. The cause of this acidification is not definitely known. A number of hypotheses may be put forward: decrease in salivary volume (Laudenbach, 6, and Vermeil, 7), excretion of acid metabolites by the salivary glands, effect of psychotropic agents upon the action itself of the salivary glands.

Antidepressive Agents↗