Biomedical subjects

B Czerniak

Publications and source records attributed to B Czerniak.

At least 55 records · Page 3Linked to original sources

PubMed1985

Flow cytometric identification of cancer cells in effusions with Ca1 monoclonal antibody.

The fluorescence of cells from 42 pleural and peritoneal effusions stained with Ca1 monoclonal antibody (Ca1MA) was studied by flow cytometry. In 14 of 17 malignant effusions a significantly higher intensity of fluorescence was observed in samples exposed to Ca1MA when compared with controls. There was no increase of fluorescence intensity in 25 benign effusions. The method failed in three malignant effusions: one due to endometrial carcinoma and two to malignant lymphoma. The sensitivity of the method was tested in experimental samples with a known percentage of malignant cells. The positive fluorescence with Ca1MA was detected in samples containing 0.1% of carcinoma cells. Flow cytometry with Ca1MA can be a relatively simple method of identification of malignant cells in effusions.

Antibodies, Monoclonal↗

PubMed1985

Expression of Ca antigen on human urinary bladder tumors.

Flow cytometric measurements of Ca antigen (Ca) visualized by immunofluorescence with Ca1 monoclonal antibody (Ca1) were performed on cells from 26 urinary bladder carcinomas of various histologic grades and DNA ploidy and on 6 samples of morphologically normal urothelium. In normal urothelium and in the 14 diploid tumors the proportion of positively staining cells was low and the differences were not statistically significant. A significant increase in the proportion of fluorescent cells was observed in all but one of the 12 aneuploid tumors. Among the 11 tumors with an increased proportion of positive cells, 7 were invasive. Data recorded in this study suggest that the quantitation of Ca1 fluorescence corresponding to the presence of Ca may prove to be helpful in the evaluation of clinical behavior of bladder tumors.

Aneuploidy↗

PubMed1984

Diagnosis of orbital tumors by aspiration biopsy guided by computerized tomography.

Thin-needle aspiration biopsy (TNAB) was performed on 22 patients with orbital tumors. Nine patients were studied before the introduction of computed tomography (CT) and only two cytologic diagnoses could be established. Thirteen aspiration biopsies guided by CT were successful. The technique of the CT-guided TNAB of orbital tumors is described. The cytologic features combined with the clinical data made it possible to diagnose precisely 10 malignant orbital tumors that were either metastatic or primary. Two meningiomas and one abscess were also identified. There were no complications. The TNAB of orbital tumors under CT guidance appears to offer several significant benefits to the patients.

Adult↗

PubMed1984

Expression of Ca antigen in relation to cell cycle in cultured human tumor cells.

In order to explain the heterogeneity of Ca antigen (Ca) expression observed previously on human malignant cells, a relationship of antigen content with events in the cell cycle was studied by multiparameter flow cytometry on four continuous human cell lines, HeLaS3, C4l, HT29, and T24, grown exponentially in culture. In the four cell lines studied, there was an increase in Ca expression during the G1 and G2 + M phases whereas, during the S phase, the Ca expression was relatively constant. Although the level of Ca varied among the four cell lines, the mean amount of Ca expressed during the G2 + M phase was from 58 to 94% higher than the mean for the G1 phase. The G1 and G2 + M cell populations also displayed considerably greater variability of Ca content when compared with cells in the S phase. The level of Ca among cell lines appeared to have an inverse relationship to DNA content expressed as DNA index. Ca expression appeared to be related to RNA content and hence, presumably, ribosomal content, across the cell cycle. This study suggests that some of the heterogeneity of Ca expression in human cancer cells may be related to cell cycle events.

Antigens, Neoplasm↗

PubMed1984

Nuclear pores and DNA ploidy in human bladder carcinomas.

The number of nuclear pores per sq micron was determined on the freeze-fractured nuclei of 20 human bladder tumors and five control samples of normal bladder epithelium. Measurements of the nuclear surface and volume were also performed, and the mean number of pores per nucleus and the ratio of pore to volume were calculated. The DNA distribution pattern on the same samples was determined by flow cytometry. All control samples and 12 tumors were diploid, and eight tumors were aneuploid. The mean number of pores per sq micron and mean total number of pores per nucleus in the control samples and in diploid tumors were similar. In the aneuploid tumors, both values were significantly higher. However, the ratio of pore to volume was shown to be constant regardless of the DNA content. It was further observed that, in aneuploid tumors, there are two populations of nuclei, one with density of pores similar to the diploid tumor and one with a higher pore density. Because aneuploid bladder tumors have been shown to have more aggressive behavior than diploid tumors, increased density of nuclear pores or their total number per nucleus may be related to tumor behavior. This view is supported by the observation that five of eight tumors with increased density and total pore number were invasive, while all tumors with low pore number were noninvasive.

DNA, Neoplasm↗

PubMed1983