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Biomedical subjects

B Cylwik

Publications and source records attributed to B Cylwik.

At least 19 recordsLinked to original sources
PubMed2003

Circulating tumour necrosis factor alpha and soluble tumour necrosis factor receptors in patients with different patterns of rheumatoid synovitis.

OBJECTIVE: To examine the relation between the serum levels of tumour necrosis factor alpha (TNFalpha), soluble tumour necrosis factor receptors (sTNF-R), and the histological pattern of rheumatoid synovitis. METHODS: An enzyme linked immunosorbent assay (ELISA) was used to measure TNFalpha, p55 sTNF-R, and p75 sTNF-R concentrations in the serum of 43 patients with rheumatoid arthritis (RA) and 34 patients with osteoarthritis (OA). RESULTS: Upon histological analysis two variants of rheumatoid synovitis emerged. Twenty six RA specimens presented only diffuse infiltrates of mononuclear cells. In the remaining 17 samples the formation of lymphocytic follicles with germinal centre-like structures was found. Serum concentrations of TNFalpha, p55 and p75 sTNF-R were raised in patients with RA compared with the OA control group (p<0.001 for all comparisons). Levels of TNFalpha, p55 and p75 sTNF-R were higher in the serum of patients with RA with follicular synovitis than in patients with diffuse synovitis (p<0.001, p<0.01, and p<0.05, respectively). Serum concentrations of TNFalpha, p55 and p75 sTNF-R correlated with markers of disease activity. CONCLUSION: Different histological types of rheumatoid synovitis associated with distinct serum levels of TNFalpha and sTNF-R reflect varying clinical activity of the disease and support the concept of RA heterogeneity.

Aged↗
PubMed2002

Serum matrix metalloproteinases and tissue inhibitors of metalloproteinases in different histological variants of rheumatoid synovitis.

OBJECTIVE: Rheumatoid synovitis is characterized by an invasive and tissue-destructive infiltrate of lymphocytes, macrophages and synoviocytes. Matrix metalloproteinases (MMPs) and tissue inhibitors of metalloproteinases (TIMPs) produced by these cells are important in the remodelling of the articular tissues in rheumatoid arthritis (RA). The aim of this study was to explore whether the serum concentrations of MMPs and their inhibitors were correlated with the histological appearance of the disease. METHODS: Tissue and serum samples were obtained from 37 patients with clinically active RA and 30 with osteoarthritis (OA). Morphological analysis allowed the division of RA synovial specimens into two distinct types. In 22 samples only diffuse infiltrates of mononuclear cells without further microanatomical organization were found. In 15 specimens we observed lymphocytic conglomerates with germinal centre-like structures. Serum concentrations of interstitial collagenase (MMP-1), stromelysin-1 (MMP-3), gelatinase B (MMP-9), TIMP-1 and TIMP-2 were measured with an ELISA technique. RESULTS: Unique serum profiles of MMPs and TIMPs were identified in each of the two histological types of RA synovitis. The serum concentrations of MMP-1, MMP-3 and MMP-9 were higher in RA patients than in OA patients used as a control group (P<0.001 for all comparisons). These three MMPs dominated in the serum of RA patients with follicular synovitis compared with those with diffuse synovitis (P<0.05, P<0.01 and P<0.001 respectively). The analysis of the serum concentrations of TIMP-1 and TIMP-2 showed that their levels were also elevated in RA patients compared with OA patients (P<0.001 and P<0.01 respectively). Only TIMP-1 was found in a significantly higher concentration in the serum of RA patients with follicular synovitis than in those with diffuse synovitis (P<0.05). The serum concentrations of MMPs and TIMP-1 clearly identified patients with two different histological types of rheumatoid synovitis and with OA. Additionally, the analysis of clinical data showed that the rheumatoid disease in patients with follicular synovitis seemed to be more active than in those with diffuse synovitis. CONCLUSION: The morphological appearance of rheumatoid synovitis and the serum MMP and TIMP-1 profile were correlated with the clinical activity of the disease, confirming the heterogeneity of RA. These associations also suggest that patients with different histological forms of RA might require different treatment regimens.

Adult↗
PubMed2002

Soluble adhesion molecules (ICAM-1, VCAM-1, and E-selectin) and vascular endothelial growth factor (VEGF) in patients with distinct variants of rheumatoid synovitis.

BACKGROUND: Cell adhesion molecules and endothelial growth factors have an important role in the infiltrating of rheumatoid synovium with mononuclear cells, leading to the initiation and progression of the disease. OBJECTIVE: To investigate whether the serum profile of soluble adhesion molecules and of vascular endothelial growth factor (VEGF) is associated with the histological appearance of rheumatoid arthritis (RA). METHODS: Serum levels of soluble intercellular adhesion molecule-1 (sICAM-1), vascular cell adhesion molecule-1 (sVCAM-1), E-selectin (sE-selectin), and VEGF were assessed by enzyme linked immunosorbent assay (ELISA) in 40 patients with RA and 32 patients with osteoarthritis (OA). RESULTS: Histological analysis of synovium specimens distinguished two types of rheumatoid synovitis. Twenty four RA samples presented diffuse infiltrates of mononuclear cells without any further microanatomical organisation, whereas in the remaining 16 samples lymphocytic follicles with germinal centre-like structures were identified. In comparison with patients with OA, constituting a control group, higher serum concentrations of sICAM-1 (p<0.001), sVCAM-1 (p<0.001), sE-selectin (p<0.01), and VEGF (p<0.001) were detected in patients with RA. Raised concentrations of sICAM-1, sVCAM-1, and VEGF dominated in the serum of patients with RA with follicular synovitis compared with those with diffuse synovitis (p<0.01 for all comparisons). The serum concentrations of sICAM-1, sVCAM-1, and VEGF correlated with markers of disease activity such as the erythrocyte sedimentation rate and C reactive protein levels. Furthermore, the clinical data analysed in our study indicated that patients with RA with follicular synovitis tend to have more severe disease. CONCLUSIONS: The distinct histological appearances of rheumatoid synovitis associated with different serum profiles of sICAM-1, sVCAM-1, and VEGF reflect varied clinical activity of the disease and confirm RA heterogeneity. Patients with different histological forms of synovitis may respond differently to the treatment regimens.

Arthritis, Rheumatoid↗
PubMed2001

Serum cytokines in different histological variants of rheumatoid arthritis.

OBJECTIVE: Rheumatoid arthritis (RA) is characterized by an invasive and tissue destructive infiltrate of lymphocytes, macrophages, and synoviocytes formed in the joints. Its etiopathogenesis and the role of the particular morphological components of synovitis remain unclear. There is evidence that its histological heterogeneity is correlated with synovium cytokine transcription. We investigated whether the serum cytokine profile is associated with the morphological appearance of the disease. METHODS: Tissue and serum samples were collected from 25 patients with clinically active RA and 25 with osteoarthritis (OA) as a control group. After histological analysis RA synovial biopsies were divided into 2 distinct types; 16 samples were characterized by diffuse lymphocyte infiltrates with no additional microanatomical organization. Lymphocytic aggregates with germinal center-like structures were found in 9 specimens. Serum concentrations of interferon-gamma (IFN-gamma), interleukin 12 (IL-12, p70 heterodimer), tumor necrosis factor-alpha (TNF-alpha), and IL-15 were measured by ELISA. RESULTS: Low concentrations of IFN-gamma (p < 0.01) and IL-12 (NS) were found in RA patients' serum compared with OA controls. RA patients with follicular synovitis had lower serum concentration of IFN-gamma (p < 0.05) and IL-12 (p < 0.05) than patients with diffuse infiltrates. High concentration of TNF-alpha and IL-15 characterized RA patient serum in comparison with controls (respectively, p < 0.001 and p < 0.01). In the serum of RA patients with follicular synovitis TNF-alpha was a dominant cytokine (p < 0.01) compared to patients with diffuse disease. At TNF-alpha level > or = 44 pg/ml, 5 (56%) of 9 patients with follicular RA had such elevated values vs one of 16 diffuse patients (< 10%; p < 0.02). Only serum concentrations of TNF-alpha could effectively differentiate between patients with OA and subgroups of RA. Analysis of clinical data suggested that activity of rheumatoid disease in patients with follicular synovitis was more severe than in those with diffuse infiltrates. CONCLUSION: The association between distinct histological appearance of rheumatoid synovitis and serum cytokine profile and diverse clinical activity of disease seems to confirm its heterogeneity.

Adult↗
PubMed2000

Morphometric and ultrastructural studies of the sciatic nerve regeneration in rats intoxicated with ethanol.

The aim of the study was to examine the process of sciatic nerve regeneration and changes in the dorsal root ganglia (from which sensory fibres of the sciatic nerve extend) in animals intoxicated with ethanol. The experiment used 20 rats, divided into two groups: control and treated. The treated animals were intragastrically given 2g/kg b.w. of ethanol in 25% aqueous solution. In both groups the right sciatic nerve was transected and then sutured. After 5 months the animals were anaesthetized. The left and the right spinal dorsal ganglia-L5 and sections from the non-operated and operated sciatic nerves were collected for analysis. Ultrastructural examinations and morphometric measurements were conducted. It was found that ethanol administrated to rats inhibited regeneration of the transected and then sutured sciatic nerve, impairing the growth of axons in the transected nerve and destroying the regenerating sensory ganglion cells. The mechanism of the changes described may be associated with axonal transport disorders or with the suppressed production of biologically active substances, which affect nerve regeneration.

Animals↗
PubMed1998

[Clinical significance of arthroscopic synovial biopsy in the diagnosis of knee synovitis].

Eighty-two patients (49 females and 33 males, mean age 38.2 years) underwent arthroscopic synovial biopsy in the course of treatment for chronic knee synovitis. After histopatological analysis of the samples Fritz et al. criteria were used for their classification. In 16 cases of specific synovitis clinical diagnosis was always confirmed by histopathology. In 66 patients with nonspecific synovitis lymphoplasmatic and serofibrous type prevailed. In 8 cases with dominant non-specific synovitis histological features of specific synovitis have been also found and correct clinical diagnosis has been established. Arthroscopic synovial biopsy increases diagnostic potential in the synovitis of unclear etiology.

Adolescent↗
PubMed1997

[Diagnosis usefulness of beta-2 microglobulin in clinical practice].

The laboratory diagnostics is mostly based on proteins determination and this tendency has been developed lately. It especially concerns the determination of the low molecular weight proteins. One of them became beta 2-microglobulin (beta 2-M), whose role in the organism and diagnostics is presently investigated. In this paper, the general characteristic of beta 2-M has been given including the usefulness in clinical diagnostics. A great part of this paper concerns role of this protein in nephrological diagnostics (early detection and localization of disorder, selective evaluation of filtration and resorption function, assessment of the degree of glomerules and tubules damage) and its significance in this field is without question. A very important problem is the determination of beta 2-M both in serum and urine, giving full information about renal dysfunction. The usefulness of beta 2-microglobulin in diagnosing cancer also has been described and discussed, and this protein can be used as a relatively sensitive marker of treatment in selected leukaemias. A beta 2-M usefulness in diagnostics has been presented in other diseases too, but it is worthy to note that the value of this test presently is not fully appreciated by clinicians.

Humans↗
PubMed1997

C-reactive protein concentration in the sera of pregnant women with imminent premature parturition and preterm amniorrhea.

C-reactive protein (CRP) levels were evaluated during spontaneous labour, in imminent premature parturition (IPP) and after preterm rupture of oocyst membranes (PROM). Increasing CRP level during spontaneous labour was found in 16.6% parturients. Elevated CRP level in patients with IPP makes it impossible to predict premature labour. In parturients with PROM, increase in CRP level makes further infection predictable only in a few cases.

C-Reactive Protein↗
PubMed1997

Ultrastructural evaluation of microcirculatory vessels in rheumatoid synovial membrane.

Since morphological lesions in microcirculatory vessels are often difficult to be found in the light microscope, the electron microscope investigations were performed on the synovial membrane biopsy specimens from 70 patients with rheumatoid arthritis (RA). Most common lesions referred to venules, capillaries and arterioles were swelling and proliferation of endothelial cells, adherence of lymphocytes, monocytes and neutrofiles to the endothelium, their margination and diapedesis. Also destructive changes in the endothelial cells, basement membrane thickening due to their multiplication and microthrombi were observed. Platelet aggregates, fibrin, fragments of desintegrated cells and deposits of granulofibrillary substance corresponding to fibrinoid necrosis were frequently seen. In 7 patients, lesions of this kind were found only in electronograms. It can be assumed that the evaluation of ultrastructural changes in the microcirculatory vessels may be of great significance as a complementary diagnostic examination in future determination of RA progression and further prognostication.

Adult↗
PubMed1995

Piroxicam and poststeroidal damage of articular cartilage.

The experiment was carried out in five groups of rats with 12 animals in each one. In three groups articular cartilage osteoarthrosis like damage was accomplished by intramuscular injection of Dexamethason once per week. The rest of animals with exception of the controls were given Piroxicam each day by gastric tube. In the group of rats receiving only Dexamethason we observed extensive destructive changes in the cartilage. In rats receiving Dexamethason and Piroxicam (mainly in the group with higher dosage) histological changes were significantly less than in the group receiving only steroid. The intensity of glycosaminoglycan (GAG) histochemical reactions were significantly higher. In the cartilage of rats receiving only Piroxicam degenerative changes were not found, and GAG contents were similar to the control group. Our results suggest an increase of GAG biosynthesis in articular cartilage after Piroxicam in experimental, poststeroidal damage.

Administration, Oral↗
PubMed1994

Von Willebrand factor antigen in assessment of vasculitis in patients with connective tissue diseases.

Von Willebrand factor antigen (vWF:Ag) is synthesized and secreted by endothelial cells. In the present study we tried to assess the relationship between plasma level of vWF:Ag and vascular damage in patients with vasculitis. The study was carried out on 59 patients with connective tissue diseases. Vasculitis was diagnosed by biopsies of the skin. The patients with vasculitis had a significantly elevated level of vWF:Ag; however, no significant correlation between the amount of plasma vWF:Ag and the degree of vasculitis was found. The obtained results show that the plasma level of vWF:Ag may reflect the presence of vascular, especially endothelial, damage in patients with connective tissue diseases.

Adult↗
PubMed1990

[Primary liver cell carcinoma among the population of Białystok in 1975-1988 in relation to hepatitis B virus infection].

In presented study, retrospective epidemiological analysis was performed in 84 patients dead due primary hepatocellular carcinoma, selected from among 17,973 persons dead in Białystok hospitals from 1975 till 1988. In the part from among these patients was estimated prevalence of serological markers of Hepatitis B virus infection, as a potential oncogenic factor. Analysis of this carcinoma diagnosis frequency in particular years showed rising tendency: from 1975 till 1979 number of PHC diagnosis was from 0 to 5 a year, whereas in 1988 it was 13. Percentage of necropsy diagnosed PHC was similar and increased to over 1% of all necropsies in 1988. Mean age of patients dead due PHC was 65, and 71.4% were men. Serological markers of HBV infection were observed in 50% and HBsAg in 34.6% from among PHC-dead patients. These percentage values were significantly higher, than observed in control group of Białystok population, which indicate possibility of association between HBV infection and PHC development.

Adult↗
PubMed1985

Morphometric investigations of dog kidney infarcts after embolization of the renal artery with Spongostan.

A macroscopic morphometric method for evaluating the volume of infarcts in the kidneys of dogs 24 hours, 7, 14, and 24 days after renal artery embolization by means of Spongostan is described. The whole kidney was not infarcted, the volume of the infarcts being 60.15-81.87% (mean 71.1%) of the volume of the embolized kidney. In order to evaluate the distant morphological changes in the parts of the embolized kidney not involved in the infarcts, it would be necessary to carry out microscopic examinations and to have a longer observation period.

Animals↗
PubMed1985

Morphometric and histological examinations of dog kidneys after embolization of the renal artery with the cyanoacrylic glue "Chirurcoll-Polfa".

The renal artery in 20 mongrel dogs was embolized with cyanoacrylic glue "Chirurcoll-Polfa", introduced through a catheter. The extent of the resulting renal infarction of the kidneys was evaluated morphometrically and was found to be 71.35 to 91.43% of the volume of the embolized kidneys (mean 77.61%). Histological examinations revealed no resorption of the glue after 3 months. In the embolized vessels, after 7 days tumefaction and degeneration of the reticulin, elastic, and collagen fibres were observed, after 24 days inflammatory changes, and after 2 and 3 months respectively, secondary collagenization and hyalinization. Inflammatory changes were also found in the perivascular tissue. In the sections of the kidneys not involved in the infarction a progressive atrophy of the nephrons and fibrosis of the parenchyma were observed 2 and 3 months after embolization.

Animals↗
PubMed1985

Histological and histochemical changes in the dog kidney after renal artery embolization with Spongostan.

Histological and histochemical examinations of canine kidneys 24 hours, 7, 14 and 24 days after embolization of the renal artery with Spongostan showed that during 24 days the resorption and organization of the infarcts in the embolized kidneys and the resorption of Spongostan in the arteries were not yet completed. The intensified reactions to phosphatases, dehydrogenases, and non-specific esterase indicate a prolonged activation of cells playing a role in the resorption and organization of infarcts which may be of significance in the stimulation of immune responses. There were no inflammatory reactions in the embolized arteries.

Animals↗
PubMed1985

[Effect of embolization of the renal artery on histologic changes in primary kidney cancer].

The authors compared the histologic changes in 12 kidneys removed due to renal carcinoma with the changes in 12 other kidneys in which before nephrectomy an embolization of the renal artery with Spongostan had been performed. The results achieved show that the embolization of the renal artery may intensify the lymphocytic macrophagal and fibroplastic interstice of the tumour and according to this may represent a factor which is able to activate the cellular effector mechanism of the antineoplastic immunity of the organism.

Adult↗