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Biomedical subjects

B Combes

Publications and source records attributed to B Combes.

At least 55 records · Page 3Linked to original sources
PubMed1976

Biliary excretion of dye in dogs infused with BSP or its glutathione conjugate.

A comparison of the maximal rates of biliary excretion (Tm), of dye in dogs infused with either BSP or its glutathione conjugate (BSP-GSH) was carried out. Tm was much higher when BSP-GSH rather than BSP was infused. This was accounted for by a significantly higher concentration of dye in bile of dogs receiving BSP-GSH. Evidence is presented that BSP and its conjugated metabolites compete for a common transport carrier and that BSP disproportionately depresses the biliary excretion of conjugated dye compounds. This latter observation accounts for the depressed dye Tm found during infusion of BSP. Choleresis invariably accompanied dye excretion. When BSP-GSH was infused, enhanced bile flow could be accounted for by the predicted osmotic activity of dye transported into bile. By contrast, the choleresis measured during infusion of BSP was significantly greater than that predicted. An additional mechanism for choleresis is operative, therefore, when unconjugated BSP is infused. Administration of taurocholate enhanced dye Tm when BSP-GSH was infused. Since increments of canalicular bile flow induced by theophylline and glucagon did not enhance dye excretion into bile, this effect by taurocholate appears to be related to taurocholate excretion per se rather than to the enhanced canalicular bile flow which accompanies its excretion.

Animals↗
PubMed1976

Solution properties of sulfobromophthalein sodium (BSP) compounds alone and in association with sodium taurocholate (TC).

A series of in vitro studies have been performed utilizing the techniques of ultracentrifugation, freezing point depression, vapor pressure osmometry, and spectrophotometry, to study the colloid-chemical characteristics of various sulfobromophthalein sodium (BSP) compounds in aqueous solution and to evaluate the possibility of a direct physicochemical interaction between BSP and taurocholate (TC). The results of these studies indicate that: (1) BSP compounds self-associate in aqueous solution to form polymolecular aggregates. These aggregates are larger with conjugated BSP, where the aggregation number appears to increase with the concentration of BSP, compared with the more polar glutathione conjugate of BSP; (2) There is a marked physicochemical interaction between unconjugated BSP and TC and a much smaller effect between the bile salt and conjugated BSP. This interaction was minor between BSP and glycodeoxycholate or taurodehydrocholate but was reproduced fully by glycocholate. Such an interaction between BSP and TC may have physiologic importance and may help to explain the previously noted facilitated excretion of BSP observed after infusion of TC in experimental animals.

Journal Article↗
PubMed1975

Serum gamma-glutamyl transpeptidase activity in normal children.

Serum gamma-glutamyl transpeptidase (GGT) activities of 82 healthy neonates (aged 9 hours to 11 days) and 106 healthy children (aged 2 months to 15 years) were determined. Serum GGT activity of 47 neonates (51%) was higher than the accepted upper limit of normal for adults. By three months of age, all of the children had serum GGT activities that were within the accepted normal range for adults. Thereafter there was only minimal variation in serum GGT activities of older children. Although mean serum GGT activity was higher in male children than in female children, there was no significant difference between the values for male and female neonates. That after the neonatal period serum GGT activity is constant in the adult range and is not affected by bone growth as is alkaline phosphatase suggests that GGT may be of value in the evaluation of hepatobiliary disease in children.

ABO Blood-Group System↗
PubMed1975

Prognostic significance of subacute hepatic necrosis in acute hepatitis.

A retrospective analysis has been made of 57 patients with subacute hepatic necrosis demonstrated on a liver biopsy obtained during the course of an episode of acute hepatitis. Fourteen patients have been lost to follow-up. One patient died acutely with massive hepatic necrosis, while 8 have developed chronic active liver disease. Two of nine biopsies subsequently performed on patients who had shown complete clinical and biochemical resolution revealed an inactive postnecrotic cirrhosis. The incidence of these complications developing in patients with subacute hepatic necrosis was approximately 30%. These findings add qualitative support to the position that liver biopsy findings bear important prognostic value in patients with acute hepatitis.

Acute Disease↗