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B Cinader

Publications and source records attributed to B Cinader.

172 records · Page 10Linked to original sources
PubMedDate not supplied

Alcohol dehydrogenase is not a major determinant of alcohol preference in mice.

We present evidence that in MRL/Mp mice, difference in one gene affects the activity of alcohol dehydrogenase. MRL/Mp- + show high alcohol dehydrogenase (ADH) activity in the range of that found in C57BL/6, while MRL/Mp-lpr show ADH values in the range of non-drinker strains such as DBA/1, BALB/c, A, SJL, CBA and C3H/He. Voluntary alcohol consumption in MRL/Mp- + is 30-40% higher than that in the MRL/Mp-lpr. However, alcohol preference of both congenic strains is markedly lower than those of C57BL/6 mice. Our findings lead us to conclude that although alcohol dehydrogenase levels do relate to alcohol preference in mice, they are responsible for only a minor fraction of inter-strain differences.

Alcohol Dehydrogenase↗
PubMedDate not supplied

Sensitization of T and B cells by a normally tolerogenic macromolecule: the induction of unresponsiveness and of sensitization is polymorphic.

Changes induced in B and T cells by tolerogen were experimentally defined in animals which resist down-regulation. Female A/J, C57BL/6J, MLR/MpJ-lpr/lpr, MLR/MpJ-+/+ and NZB/B1NJ mice were injected at various ages with a tolerogenic form of rabbit gamma globulin (sRGG) or were left uninjected, and all were then immunized with dinitrophenylated RGG (DNP-RGG) on alum. The degree of tolerance was estimated by measuring anti-DNP and anti-RGG spleen plaque-forming cell (PFC) numbers. In some cases, the state of T or B cells deduced from these experiments was further examined by cell transfer experiments. Four types of responsiveness to the tolerogenic form of RGG (sRGG) were distinguished: 1) persistent tolerance inducibility of T and B cells to tolerance induction (A/J); 2) T cells retaining tolerance-inducibility after initiation of age-dependent sensitization of B cells by the tolerogenic form of RGG (C57BL/6J, NZB/B1NJ); 3) age-dependent resistance of T cells to tolerance-induction and age-dependent sensitization of B cells by tolerogen, with the sensitization only manifesting itself in reactivity with T cells from immunized donors (SJL/J); and 4) sensitization of T cells by a usually tolerogenic form of RGG (MLR/MpJ-lpr-lpr). Thus, the development of resistance against tolerance induction is highly polymorphic, not only with respect to the age of onset, but also with respect to the cellular site of its first manifestation and the effect. Possible mechanisms of B-cell sensitization in sRGG-treated C57BL/6 and NZB mice are discussed in relation to the age-dependent emergence of a B-cell subpopulation and of non-specific helper T-cell factors.

Aging↗
PubMedDate not supplied

Rabbit B spleen lymphocytes and macrophages as accessory cells in T-cell activation by mitogens.

The enhancement of the response of T cells to concanavalin A (ConA) and phtyohaemagglutinin (PHA) by macrophages has been shown in most species, whereas the accessory role of B cells has been only described in studies using human or rabbit lymphocytes. In rabbit, the accessory activity is confined to a subpopulation of B lymphocytes: the majority of B cells have sedimentation velocity of 2.5 to 4 mm/h, whereas the maximum of the accessory activity is found among the B cells, sedimenting with a velocity of 3.5 to 8 mm/h; B cells sedimenting between 1 and 3.5 mm/h have only a very weak accessory activity. Splenic adherent and/or phagocytic spleen cells may contribute additional augmentation of the response of T cells to ConA, since other macrophages (peritoneal and alveolar) are able to increase the ConA response to spleen T cells.

Animals↗