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Biomedical subjects

B Cercek

Publications and source records attributed to B Cercek.

At least 109 records · Page 6Linked to original sources
PubMed1975

Induction of PHA response in mouse bone marrow cells by thymic extracts as studied by changes in the structuredness of cytoplasmic matrix.

The specificity and cross-species activity of thymic extracts and some non-specific agents on some alleged properties of T cells and their precursors in the bone marrow cells of normal, nude and thymectomized mice was studied in vitro. The phenomenon of changes in the structuredness of cytoplasmic matrix (SCM) was used to assess the maturation and responses of cells treated with the extracts to mitogens, i.e. phytohaemagglutinin and concanavalin A. We found that thymic extracts of calf and mouse origin induce functional 'maturation' which can be abrogated by cycloheximide, an inhibitor of protein synthesis. The nonspecific agents used (spleen extracts, PPD, dBcAMP and endotoxin) had no effect on bone marrow cells, as measured by changes in the SCM. The method of measuring SCM using the technique of fluorescence polarization is described.

Animals↗
PubMed1974

Inhibitors of haemopoietic cell proliferation? Specificity of action within the haemopoietic system.

The specificity of action of mature blood cell extracts on their own progenitor cells was investigated by measuring their effects on the structuredness of the cytoplasmic matrix (SCM) using the technique of fluorescence polarization. Changes in SCM induced by the various extracts are probably closely related to the proliferation state of the cells.Saline extracts of lymphocytes, granulocytes and erythrocytes (LNE, GCE and RCE respectively) have been partially purified by ultrafiltration into selected molecular weight ranges and each tested against proliferative populations of lymphoid, granulocytic and erythroid cells. In all cases, complete specificity of effect on SCM was found, LNEs affecting only lymphoid cell populations, GCEs affecting only the granulocytic cell populations and RCEs affecting only erythroid cells. In each case, with the possible exception of the RCEs, the active fractions reside in the molecular weight ranges reported in the literature for cell extracts possessing proliferation inhibitory properties.

Animals↗