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Biomedical subjects

B Caddy

Publications and source records attributed to B Caddy.

33 records · Page 2Linked to original sources

Extraction of high quality genomic DNA from microsamples of human blood.

A simple and efficient method for extracting human genomic DNA from microsamples of blood has been developed. This method used sodium perchlorate, chloroform, polymerised silica gel and a dumbbell-shape tube, instead of proteinase K and phenol. The entire process took less than two hours, and high molecular weight DNA, in high yield and purity, was obtained from a few microlitres of human blood. DNA prepared in this way can be easily digested with restriction endonucleases and has been employed for DNA profiling and the polymerase chain reaction.

DNA↗

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Forensic Medicine↗