KINETICS OF THE FERRIMYOGLOBIN-AZIDE-SYSTEM.
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Biomedical subjects
Publications and source records attributed to B CHANCE.
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The cytochrome composition of mitochondrial fractions which have been stripped of inner membrane subunits by exposure to high-frequency sound have been examined by lowtemperature spectroscopy. The ratio of cytochrome c to cytochrome a is not changed by the treatment, but the concentration of cytochrome per milligram of protein is increased and the concentrations of cytochromes c(1) and b change slightly. These cytochromes (c(1) and b) may be at least partly located in the subunits of the inner membrane, but the idea that all the respiratory components are located in single subunits of the mitochondrial cristae may be considered to be disproved.
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Projecting subunits of an average diameter of 80 A are found on the cristae of mitochondria prepared from the muscle of Ascaris lumbricoides. A spectroscopic examination of the cytochrome content of these mitochondria shows no detectable cytochrome c(1), a, or a(3) and does reveal cytochromes of types c and b. Subunits in the same size range are found in cytochrome c deficient mitochondria of the emergent bee, while the frequency of their occurrence along the cristae is decreased relative to the adult bee. Apparently, the cytochrome content of the respiratory chain is not related to the size of the subunits, but may be related to the frequency of occurrence of the subunits.
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