Search PubMed⌕ Search

Biomedical subjects

B C Starcher

Publications and source records attributed to B C Starcher.

At least 55 records · Page 3Linked to original sources
PubMed1977

Comparison of the elastolytic effects of human leukocyte elastase and porcine pancreatic elastase.

Porcine and bovine elastins were digested by human leukocyte elastase and porcine pancreatic elastase. The enzymes showed similarities in the extent to which they digested elastin and the pattern and quantitative distribution of N-terminal amino acids in the digests. However, fingerprints of the digests showed differences between the products of leukocyte elastase and pancreatic elastase. Each enzyme produced its characteristic fingerprint irrespective of whether the elastin substrate was obtained from ligament, pleura or lung parenchyma. The enzymes also digested tropoelastin differently. The results suggest that leukocyte elastase and pancreatic elastase should not be considered interchangeable in experimental models of tissue injury.

Amino Acid Sequence↗
PubMed1977

The induction of pulmonary emphysema with human leukocyte elastase.

Purified human leukocyte elastase was injected into the tracheas of 46 hamsters. Thirteen animals died spontaneously within 1 week, with extensive lung hemorrhage. The elastin content of the lungs was only slightly less than control values 3 hours after injection. At 2 months, the lungs of the remaining animals showed mild, patchy emphysema and morphometric changes consistent with emphysema. These results contrasted with the effects of a similar elastolytic dose of pancreatic elastase administered to 26 other hamsters in that only one animal died spontaneously, the lung elastin content 3 hours after injection was substantially decreased, and severe emphysema was present 2 months later. Leukocyte elastase appears to be capable of causing emphysema; but unlike pancreatic elastase, leukocyte elastase produces emphysema that is mild, even at a dose sufficient to produce intense lung hemorrhage and a high mortality.

Animals↗
PubMed1975

Scanning electron microscopy and electron probe microanalysis of calcified alpha-elastin coacervates.

The uptake of calcium and phosphorus from a serum calcification medium by coacervated alpha-elastin was studied by electron probe microanalysis and scanning electron microscopy. Calcium and phosphorus were bound by the coacervate in ratios similar to that of hydroxyapatite. A significant difference was observed in the secondary electron image of the coacercules from the serum during calcification of the coacervate.

Animals↗
PubMed1975

A large scale procedure for purification of desmosine and isodesmosine.

A rapid large scale procedure was devised for the purification of desmosine and isodesmosine from ligamentum nuchae elastin. The method makes use of the hydrophilic nature of the desmosines which preferentially absorbs to cellulose fibers in mixtures of organic solvents. Resolution of the isomers was achieved on a polystyrene resin column.

Amino Acids↗