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Biomedical subjects

B Brinkmann

Publications and source records attributed to B Brinkmann.

At least 109 records · Page 6Linked to original sources

A sampling approach to biases in conditional probability judgments: beyond base rate neglect and statistical format.

Conditional probability judgments of rare events are often inflated. Early accounts assumed a general deficit in using statistical base rates. More recent approaches predict improvement when problems are presented in frequency format or refer to natural categories. The present theory focuses on sampling processes. Experiment 1 showed that a seeming advantage of frequency over probability formats is due to a confounded factor, the need to mentally transform stimulus samples. An information search paradigm was used in Experiment 2. When sampling by the predictor, the probability to be estimated, p(criterion/predictor), was conserved in the samples and judgments were quite accurate. However, when sampling by the criterion, the low base-rate event was strongly overrepresented, accounting for the entire bias. Judgments were quite sensitive to the sampled data, but failed to take sampling constraints into account, as shown in Experiments 3 and 4.

Adult↗

Automated fluorescent detection of a 10 loci multiplex for paternity testing.

The discovery of new highly efficient tetra repeat STR loci, development of fluorescence multicolour dye technology and capillary electrophoresis have made it possible to amplify ten loci in a single reaction. This combination provides an extraordinary effectiveness of simultaneous amplification and detection. With this method it became possible to determine individual identity and paternity at an enhanced level of precision and accuracy in 1 to 2 days with a high biostatistical probability. This review demonstrates the role of automated fluorescent multicolour dye genotyping technology in forensic paternity testing.

Electrophoresis, Capillary↗

Human identity testing with PCR-based systems.

Large numbers of repetitive stretches of DNA are present within the human genome that are associated with human individuality due to their polymorphic character. Approximately one-third of these repeat sequences is arranged as microsatellites or short tandem repeats (STRs) whose valuable application as state-of-the-art technique in human identity testing will be briefly summarized in this review. Prerequisites for successful DNA typing using STRs amplified by polymerase chain reaction (PCR) are outlined and particular attention is paid to the molecular structure of STRs from autosomes as well as from the Y chromosome. A comprehensive overview about current and emerging methods of STR analysis is given as well.

Genetic Variation↗

Immunoaffinity extraction of morphine, morphine-3-glucuronide and morphine-6-glucuronide from blood of heroin victims for simultaneous high-performance liquid chromatographic determination.

The development of an immunoaffinity-based extraction method for the determination of morphine and its glucuronides in human blood is described. For the preparation of an immunoadsorber, specific antisera (polyclonal, host: rabbit) against morphine, morphine-3-glucuronide and morphine-6-glucuronide were coupled to 1,1'-carbonyldiimidazole-activated tris-acrylgel and used for immunoaffinity extraction of morphine and its glucuronides from coronary blood. The resulting extracts were analysed by HPLC with native fluorescence detection. The mean recoveries from spiked blood samples were 71%, 76% and 88% for morphine, morphine-3-glucuronide and morphine-6-glucuronide, respectively. The limit of detection was 3 ng/g blood and the limit of quantitation was 10 ng/g blood for all three analytes. The results of the analysis of coronary blood samples from 23 fatalities due to heroin are presented.

Chromatography, Affinity↗

The role of clinical forensic medicine in cases of sexual child abuse.

The work of specialists in forensic medicine in those cases of child abuse that result in the killing of a child is defined and well known. It is less well defined in cases of (suspected) sexual abuse. The cases presented show the difficulties that arise if medical doctors and prosecutors are uncertain about the procedures that have to be followed or do not appreciate the value of objective findings. It is concluded that knowledge about necessary examinations by physicians, police officers and prosecutors has to be promoted in order to improve handling and (legal) outcome of these cases.

Child Abuse, Sexual↗

D18S535, D1S1656 and D10S2325: three efficient short tandem repeats for forensic genetics.

Three short tandem repeat (STR) polymorphisms characterized by PCR product length < 175 bp were investigated. D18S535 and D1S1656 contained a 4 bp unit as basic repeat motif, D10S2325 a 5 bp unit. The heterozygosity rates were 0.76 (D18S535), 0.88 (D10S2325) and 0. 90 (D1S1656), leading to a combined discrimination power of 0.9999. In contrast to D10S2325 and D18S535, which showed a homogeneous repeat array without any variation in the repeat motifs, repeat length and sequence variation was found for D1S1656. Robust typing results could be observed for all three STRs using highly degraded DNA.

Alleles↗

[Confusing tissue samples: causes, consequences, prevention].

The rising numbers of tissue samples for securing a diagnosis has led to a multitude of tissue samples in pathology institutes. Criteria and measures for ensuring the quality of histological diagnosis have been developed. Corresponding rules for the organization of the tissue sampling up to their processing do not yet exist. Suspicion of interchanged samples occurs much more often than it actually happens; however, it is important to be aware of the possibility so as to inform the clinician and to eliminate any possible causes of the confusion. The civil and penal consequences of such an interchanging, the subsequent "incorrect" diagnosis and the therapeutic measures are secondary compared to the potentially dramatic consequences for the patient. Important causes for an interchanging and possible preventive measures are presented.

Clinical Laboratory Techniques↗

Y-Chromosomal STR haplotypes in a population from north west Germany.

We present a German Y-chromosome short tandem repeat (STR)-haplotype database consisting of the loci DYS19, DXYS156-Y, subtypes of DYS389, DYS390, DYS391, DYS392 and as well as DYS393. 104 haplotypes were observed in 179 unrelated Germans, the haplotype diversity is 98.06%. This database is a prerequisite for the forensic application of these new markers.

Chromosome Mapping↗

Influence of soil storage and exposure period on DNA recovery from teeth.

A study was performed to determine the influence of garden soil on the deoxyribonucleic acid (DNA) recovery from teeth depending on the duration of storage. In the first series 24 teeth supplied by dentists were exposed to garden soil storage for a maximum of 18 weeks. Selected samples were excavated for DNA extraction at time intervals of 6, 12 and 18 weeks. For the second series 20 teeth were stored for one year in garden soil. Following phenol/chloroform extraction with decalcification (first series) and without decalcification prior to extraction (second series) DNA was quantified, amplified using the polymerase chain reaction (PCR) for the tandem repeat loci D1S80, tyrosine hydroxylase, intron 1 (TH01) and Von Willebrand factor, intron A (VWA) (first series), human alpha fibrinogen (FGA) (second series) and sequenced in the hypervariable regions 1 and 2 (HV1, HV2) of the mitochondrial DNA (second series). The DNA concentration of the extracts after the first 6 weeks in soil was reduced by more than 90%. Amplification and direct sequencing of HV1 and HV2 of the mitochondrial genome was the most successful DNA technique.

Blood Stains↗

Human Y-chromosomal STR haplotypes in a Kurdish population sample.

In an Iraqi Kurdish population sample (n = 101), seven polymorphic STR loci of the Y-chromosome (DYS19, 389, 390, 391, 392, 393, and DXYS156-Y) were typed, with DYS389 being subtyped for its four segments. The haplotype diversity was 97.83% and 82 different haplotypes were observed. The Kurds shared some Y-types with neighbouring south Turks but strikingly few with Germans: it is 20-30 times more likely to find a sequence match in a random pair of Kurds than in a random Kurd-German pair.

Ethnicity↗

Physical activity following fatal injury from sharp pointed weapons.

Cases of suicide from sharp pointed weapons (n = 12) witnessed by one or more persons are reported with regard to the potential for physical activity. One case each involved the ulnar artery, the great saphenous vein and the periphery of the lung and liver and the physical activity following these injuries lasted for several hours. In one case, the left carotid and vertebral arteries were transected and the physical activity lasted for approximately 10 s. An extraordinary case involved a protracted incapacitation due to heart tamponade from a small myocardial injury caused by a cannula. In the remaining seven cases, a stab wound to the heart was present. With regard to the physical activity, a long-term group (2-10 min, n = 4) can be differentiated from a short-term group (approximately 10 s, n = 2) and one case of immediate incapacitation. The size of the myocardial perforation was 7-10 mm in length in the long-term group compared to 1.4-2 cm in the short-term group. So small perforations of the heart or incisions of the carotid artery offer a potential for considerable physical activity. Large perforations of the heart or a transection of the carotid and vertebral arteries can result in short-term activity.

Adult↗

Human Y-chromosomal STR types in north Thailand.

A north Thai Y-haplotype database consisting of the loci DYS19, DXYS156-Y, DYS390, DYS391, DYS392, DYS393, and the four subsegments of DYS389 is presented. We observed 44 Y-types in 50 unrelated Thais, and the haplotype diversity was calculated to be 97.36%. No Y-types were shared with a sample of 55 Japanese, but 3 matches were found in a sample of 61 Han Chinese, and there was one Thai-German match in a larger west German sample (n = 179).

Gene Frequency↗

Inflammation of the cardiac conduction system in a case of hyperthyroidism.

A 37-year-old female showed signs of hyperthyroidism 2 weeks before death after a partial thyroidectomy was carried out 15 years previously. An examination 3 days before death revealed a normal blood cell count, an increased level of thyroidal hormones, sinus tachycardia and a high blood pressure of 170/90 mm Hg. A hyperthyroidism was diagnosed and therapy with carbimazol (2 x 10 mg) was started but 2 days later fever and chill occurred and before death short phases of unconsciousness and dyspnoea. The autopsy findings showed an interstitial inflammation of the AV-node, the His-bundle and its branches which can correlate with typical ECG changes in hyperthyroidism.

Adult↗

Mitochondrial DNA typing from human axillary, pubic and head hair shafts - success rates and sequence comparisons.

The analysis of mitochondrial DNA (mtDNA) from shed hairs has gained high importance in forensic casework since telogen hairs are one of the most common types of evidence left at the crime scene. In this systematic study of hair shafts from 20 individuals, the correlation of mtDNA recovery with hair morphology (length, diameter, volume, colour), with sex, and with body localisation (head, armpit, pubis) was investigated. The highest average success rate of hypervariable region 1 (HV 1) sequencing was found in head hair shafts (75%) followed by pubic (66%) and axillary hair shafts (52%). No statistically significant correlation between morphological parameters or sex and the success rate of sequencing was found. MtDNA sequences of buccal cells, head, pubic and axillary hair shafts did not show intraindividual differences. Heteroplasmic base positions were observed neither in the hair shafts nor in control samples of buccal cells.

Adult↗

Expanding the forensic German mitochondrial DNA control region database: genetic diversity as a function of sample size and microgeography.

Mitochondrial DNA control region sequences were determined in 109 unrelated German Caucasoid individuals from north west Germany for both hypervariable regions 1 (HV1) and 2 (HV2) and 100 polymorphic nucleotide positions (nps) were found, 63 in HV1 and 37 in HV2. A total of 100 different mtDNA lineages was revealed, of which 7 were shared by 2 individuals and 1 by 3 individuals. The probability of drawing a HV1 sequence match within the north west Germans or within published sets of south Germans and west Austrians is similar (within a factor of 2) to drawing a sequence match between any two of these three population samples. Furthermore, HV1 sequences of 700 male inhabitants of one village in Lower Saxony were generated and these showed a nearly linear increase of the number of different haplotypes with increasing number of individuals, demonstrating that the commonly used haplotype diversity measure (Nei 1987) for population samples tends to underestimate mtDNA diversity in the actual population.

Austria↗

Manipulated radiographic material--capability and risk for the forensic consultant?

As interest is being increasingly focused on the digital processing of radiographs for identification of the deceased, the benefits and risks of electronic image processing are presented. With digitization of all kinds of radiographic equipment being on the increase and image processing personal computers being readily accessible, increasing quantities of manipulated radiographic material are to be expected in the future. This potential risk is meanwhile highlighted from the legal aspect.

Age Determination by Teeth↗