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Biomedical subjects

B Baranowska

Publications and source records attributed to B Baranowska.

At least 73 records · Page 4Linked to original sources
PubMed1983

[Interdependence between hormonal disorders, stage of development and degree of malignancy of prostatic cancer. 1].

The aim of this study was to determine whether in human clinical practice destruction of the prostate gland by a malignant process is accompanied by a fall in serum testosterone and dihydrotestosterone levels. Mean serum testosterone and dihydrotestosterone levels were significantly lower and LH levels notably higher in patients with metastases than in those without metastases. No notable differences were found between serum testosterone and dihydrotestosterone levels in relation to the degree of malignancy of the tumours. Concentrations of relatively inactive androgens (DHA and delta-4-androstenedione) in the serum were normal. The fall in serum concentration levels with later increase in LH levels is probably caused by malignant destruction of the prostate gland.

Acid Phosphatase↗
PubMed1982

Kinetic properties of human muscle pyruvate kinase.

The steady-state kinetics of human skeletal muscle pyruvate kinase (MA) and its RNA-complex (MB) has been examined and compared. Kinetic studies revealed significant differences in kinetic properties with respect to free and complex form of pyruvate kinase. The MA form follows a simple Michaelis-Menten kinetics in contrast with the MB form, which displays a negative cooperativity with respect to ADP. Vmax for the complex is 40-60% that for free enzyme. Heterologous RNA is a noncompetitive inhibitor of free enzyme but the kinetics of the complex (MB) is not affected. In presence of 1.0 mM ATP in an assay mixture the kinetic constants of the complex were unchanged except for Vmax, which increased by nearly 60%. Aged preparations of free enzyme (MA) were activated by 100% and more, but the native enzyme was inhibited by 22%. Inorganic phosphate is a potent activator of both forms of pyruvate kinase. In presence of 50 mM K-phosphate the apparent Michaelis constant and interaction coefficient are unchanged, but Vmax for free enzyme increases by 35% and for the complex by 70%, respectively. The specific activity of aged MA form can be restored to the original value after incubation of the enzyme in 50 mM K-phosphate, pH 7.6, or by addition of ATP (1.0 mM) to the assay mixture.

Enzyme Activation↗
PubMed1982

The role of sex hormones in the mechanism of inhibited LH release in female patients with anorexia nervosa.

In order to elucidate the mechanism of impaired LH secretion, 60 female patients with anorexia nervosa were investigated. A control group consisted of 14 women of the same age, examined in the follicular phase of the menstrual cycle. The serum LH, FSH, prolactin, TSH, testosterone, dihydrotestosterone, dehydroepiandrosterone, delta 4-androstenedione, oestrone, oestradiol, oestriol, progesterone, thyroxine, triiodothyronine, reverse T3 and serum sex hormone binding globulin (SHBG) concentrations were measured. The results showed a significant increase in serum dehydroepiandrosterone, testosterone, oestriol and reverse T3 concentrations. However, oestrone, oestradiol, progesterone, SHBG and triiodothyronine levels were significantly lower than those of the control group. The mean serum LH concentration in patients with anorexia nervosa before and after LRH stimulation was significantly lower than that in the control group, but FSH secretion in response to LRH was normal. All hormonal changes in anorexia nervosa disappeared after weight gain during cyproheptadine treatment. Dramatically increased dehydroepiandrosterone levels suggest that the high testosterone in women with anorexia nervosa is derived from adrenal rather than from gonadal steroids. There was no correlation between serum testosterone, dehydroepiandrosterone, oestriol and LH concentrations indicating that steroid hormone disturbances do not cause impaired LH release in anorexia nervosa.

Adolescent↗
PubMed1982

The role of computed tomography in the localization of adrenal cortex tumors.

The aim of this study was to evaluate the usefulness of computed tomography in the localisation of adrenal tumors producing aldosterone and cortisol. One case each of Conn's and Cushing's syndrome are described. The diagnosis of Conn's syndrome was established by demonstrating an elevated plasma aldosterone level "at rest" and its decrease after stimulation, the absence of plasma renin activity and a lowered plasma potassium level. The diagnosis of Cushing's syndrome due to adrenal adenoma was established by demonstrating the typical clinical features, an abnormal diurnal rhythm of cortisol and ACTH secretion and an increased urine excretion of 17-OHCS without suppression by large doses of dexamethasone. The localisation and the size of the tumors as determined by computed tomography were confirmed during surgery.

Adenoma↗
PubMed1981

[The influence of spironolactone on the concentration of gonadotrophins and gonadal hormones in prostatic hypertrophy (author's transl)].

The authors examined the influence of spironolactone on the concentration of testosterone, 5 alpha - dihydrotestosterone (DHT), progesterone, oestradiol (E2), LH, and FSH in 47 patients with prostatic hypertrophy, aged from 60 to 80 years. The control group consisted of 58 men of the same age. Spironolactone was prescribed at a dose of 100 mg per day for three months. There was a considerable fall in the concentration of testosterone and of DHT and, at the same time, an increase in the concentration of progesterone, E2 and LH. After treatment with spironolactone there was a decrease in the size of the prostate gland. Results obtained show that spironolactone is an effective drug in the treatment of prostatic hypertrophy, since it inhibits androgen metabolism.

Aged↗
PubMed1980

[Hormonal disturbances in men with a prostatic adenoma (author's transl)].

On the basis of a series of hormone estimations in elderly men with a prostatic adenoma, and in men not suffering from the condition (old or young), the authors attempt to develop a physiopathological theory of the pathogenesis of prostatic adenoma. The elderly subject not suffering from a prostatic adenoma has lower blood testosterone, DHT (dihydrotestosterone), LH, FSH and oestrogen levels than the elderly subject with an adenoma, whilst the latter has a lower serum progesterone level. In patients with an adenoma, all the urinary metabolites of testosterone are excreted at markedly higher levels than normal. After prostatectomy, mean serum testosterone and DHT levels fall. In patients undergoing surgery for prostatic adenoma there is a higher level of testosterone and DHT in prostatic venous blood than in the peripheral blood. In prostatic adenoma sufferers, the injection of LHRH results in a higher secretion of LH and FSH than in the control group, but this phenomenon caused an increase in testosterone level only in the controls and not in the adenoma patients. Finally, in all patients with a prostatic adenoma in the absence of stimulation by LHRH, blood testosterone levels were proportional to LH levels. Pathogenic hypothesis: the initial phenomenon could be testicular atrophy in the elderly resulting in the active secretion of gonadotrophins, which themselves would cause a hypersecretion of testosterone and its derivatives within the prostatic tissue itself. It remains to obtain evidence of the existence of gonadotrophins receptor in the prostate, the insensitivity of testicular tissue in the elderly subject to increased gonadotrophin levels and the reasons for which the increased secretion of testosterone by adenomatous prostatic tissue does not in its turn induce inhibition of pituitary hypersecretion of gonadotrophins.

Adult↗
PubMed1979

Enhanced testosterone in female patients with anorexia nervosa: its normalization after weight gain.

In order to elucidate the mechanism of disturbances of gonadal hormones secretion in anorexia nervosa 14 female patients were investigated. A control group also consisted of 14 women of the same age. The serum LH, progesterone, oestrogens: oestrone + oestradiol (Oe1 + Oe2), oestriol (Oe3) and testosterone were determined by radioimmunological methods. In patients with anorexia nervosa the serum testosterone and Oe3 concentrations were dramatically elevated, whereas LH, progesterone and Oe1 + Oe2 were decreased as compared with the control group. Considerable weight gain induced by cyproheptadine treatment caused a normalization of the serum testosterone and Oe3 concentrations in all the patients. A negative correlation between the testosterone level and the deficit in body weight was observed. The mechanism causing the dramatically high serum testosterone concentration in the female patients with anorexia nervosa is discussed.

Adolescent↗
PubMed1979

Conversion of 3H-testosterone to dihydrotestosterone in human hypertrophic prostatic tissue.

The aim of the study was to develop a simple method for the determination of the conversion of testosterone to 5 alpha-dihydrotestosterone (5 alpha-DHT) after incubation of human hypertrophic prostatic tissue with 3H-testosterone. The mean conversion rate of 3H-testosterone to 5 alpha-DHT in hypertrophic prostatic tissue was found to be higher than in normal and carcinomatous tissue. The results indicate that androgen metabolism in the hypertrophic prostatic gland is enhanced.

Aged↗