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Biomedical subjects

B Bai

Publications and source records attributed to B Bai.

22 records · Page 2Linked to original sources

[The effect of intrathecal injection of neurotensin on acupuncture analgesia in rats].

The influence of intrathecal injection of Neurotensin (NT) and Anti-NT serum (ANTS) on pain threshold, and electroacupuncture (EA) analgesia in the rat was investigated. The tail-flick induced by potassium iontophoresis was used to measure the pain threshold. The increase of pain threshold was observed within 100 min, after NT injection (2 micrograms), and it was more effective than that of the ACSF injection (P < 0.01). The NT administration could also enhance the EA analgesia. On the contrary, the pain threshold was decreased in ANTS group as compared with that of ACSF control group. The ANTS administration could decreased the role of EA analgesia. Our data show that NT in spinal cord may play an important role in the EA analgesia.

Acupuncture Analgesia↗

Electron spin resonance determination and superoxide dismutase activity in polymorphonuclear leukocytes in congestive heart failure.

Electron spin resonance spin trapping technique was used to measure the generation of active oxygen free radicals during the respiratory burst of phorbol myristate acetate-stimulated leukocytes, and the superoxide dismutase activity in healthy subjects and in patients with congestive heart failure. The authors also measured the concentration of peroxidation products (primarily malondialdehyde) by the thiobarbituric acid method. Experimental results showed that the electron spin resonance spectra obtained during the respiratory burst of polymorphonuclear leukocytes stimulated with phorbol myristate acetate primarily were those of the spin adduct of 5,5-dimethyl-1-pyrroline-N-oxide (DMPO) with the superoxide anion and to a lesser extent those of DMPO with hydroxyl radical. Compared with healthy subjects, the release of oxygen free radicals in the respiratory burst of polymorphonuclear leukocytes stimulated with phorbol myristate acetate and the concentration of thiobarbituric acid reactive product in plasma were significantly increased in patients with congestive heart failure while the activity of superoxide dismutase was markedly lower. The increased production of oxygen free radicals by polymorphonuclear leukocytes and the decreased capability of antioxidative defences might play an important role in the generation and development of cardiac failure.

Adult↗

Ventromedial hypothalamic somatostatin may affect gastric somatostatin concentration in rats.

Changes in the somatostatin-like immunoreactivity (SLI) concentrations in gastric antral mucosa were detected by RIA following microinjections of synthetic somatostatin (SS) or cysteamine (CSH) into the ventromedial nucleus of the hypothalamus (VMH). SLI concentrations in the antral mucosa were increased by 60.8% (p less than 0.001) and 42.3% (p less than 0.05), respectively, one and four hours after microinjection of somatostatin (0.5 microgram/0.5 microliter) into the VMH, and decreased by 32.6% (p less than 0.05) four hours after microinjection of cysteamine (15 microgram/0.5 microliter) into the VMH. Moreover, microinjection of somatostatin (0.5 microgram/0.5 microliter) into the VMH could only elicit an increase of 16.0% (p less than 0.05) in the SLI concentration in the antral mucosa one hour after bilateral subdiaphragmatic vagotomies, but still produced an increase of 120.0% (p less than 0.05) following celiac ganglionectomies. In conclusion, somatostatin (exogenous and endogenous) in the VMH seems to affect the gastric somatostatin levels via the vagal nerves.

Animals↗