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Biomedical subjects

B Bültmann

Publications and source records attributed to B Bültmann.

At least 91 records · Page 5Linked to original sources
PubMed1975

Migration inhibition of peritoneal macrophages by peptidoglycan.

Previous studies have shown that peptidoglycans from group A and B streptococci inhibit the migration of peritoneal exudate cells from non-sensitized rats and guinea pigs. In the present studies peptidoglycans from S. aureus and S. epidermidis were found to inhibit the migration of cells to the same extent as group A streptococcal peptidoglycan. In contrast, HSA-pentapeptide, an immunologically active synthetic analog of the peptide moiety of peptidoglycan which is free of the intrinsic toxicity of naturally occurring peptidoglycans, did not induce migration inhibition of peritoneal exudate cells from non-sensitized guinea pigs. Sensitization of animals with 200 mug HSA-pentapeptide emulsified in incomplete Freund's adjuvant significantly reduced the inhibitory effect of streptococcal and staphylococcal peptidoglycan; HSA-pentapeptide again showed no activity. However, when HSA-pentapeptide was tested against cells from animals sensitized with 200 mug HSA-pentapeptide incorporated in complete Freund's adjuvant, a strong inhibition of migration was evident. Skin tests performed in these animals, in contrast to the dermonecrotic reaction elicited by streptococcal or staphylococcal peptidoglycan, revealed a characteristic delay hypersensitivity to HSA-pentapeptide.

Animals↗
PubMed1975

Adjuvancy of streptococcal nucleic acids.

A study was performed to find out whether or not RNA and DNA isolated from Group A streptococcal cytoplasm does possess adjuvant activity. The findings obtained indicate that the adjuvancy of streptococcal RNA is very similar to that of poly I:C. The adjuvant activity of poly I:C is characterized by its capacity to increase the number of pre-existing hemolysin-producing spleen cells and by its ability to increase the development of 19S and 7S producers early in the 12-day-period of primary immune response. The adjuvancy of streptococcal DNA was considerably less pronounced than that of RNA. Neither poly I:C nor streptococcal RNA and DNA were found to be capable of increasing the process of priming for the secondary immune response.

Adjuvants, Immunologic↗
PubMed1973

Inhibition of macrophage migration by nucleotide-containing streptococcal preparations.

Certain extracts of streptococcal cell walls are known to inhibit macrophage migration in vitro. In this study, we attempted to identify the streptococcal components responsible for this phenomenon. Trypsinized cell walls and cytoplasm from groups A and B streptococci were extracted with hot formamide followed by acetone precipitation. Subsequent gel filtration in aqueous solutions yielded a fraction devoid of C-carbohydrate and containing mostly oligonucleotides, apparently derived from streptococcal cytoplasm. This fraction significantly inhibited the migration of peritoneal exudate cells from rats sensitized to groups A and B streptococci. It was noteworthy that no inhibition of migration was observed with cells from nonsensitized animals or control rats injected with BCG or complete Freund adjuvant. Similarly, no inhibition was obtained with formamide extracts of calf thymus RNA. Although the inhibition does not show specificity for streptococcal groups, it seems to have immunological specificity since prior sensitization with streptococci is required for migration inhibition.

Animals↗