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Biomedical subjects

B Astedt

Publications and source records attributed to B Astedt.

At least 109 records · Page 6Linked to original sources
PubMed1982

Immunoradiometric quantification of tissue plasminogen activator secreted by fetal organs. Comparison with urokinase.

Explants of fetal tissues were maintained in organ cultures for 2-3 weeks and the conditioned culture media analysed for the two main plasminogen activators, tissue activator and urokinase. A new immunoradiometric method was used for determining the tissue activator. The method is based on 125I-labelled antibodies to a tissue plasminogen activator which was purified from the culture medium of an established melanoma cell line. It detected tissue activator in a concentration of 1 microgram/1 or even less. Urokinase was measured with a RIA. Explants of kidney as well as thyroid gland and thymus released very substantial amounts of urokinase and smaller amounts of tissue activator. Urokinase was also detected in media conditioned by skin, spleen and pancreas. Aorta explants released only the tissue activator. The capacity of many tissues to release urokinase indicates that this is a more significant plasminogen activator in extrarenal organs that hitherto realized. The tissue activator is probably confined to vascular structures.

Animals↗
PubMed1981

Estrogen receptors in the human female lower uninary tract.

Cytosolic and nuclear fractions prepared from the urethra, urinary bladder, and trigonum of the bladder obtained at urethrocystectomy from four female patients were analyzed for the presence of estrogen receptors. High-affinity estradiol receptors (KD 0.7 x 10(-9)M) could be detected in both cytosolic and nuclear fractions of the urethra from all four patients. Estradiol receptors could be detected in only the nuclear fractions of the urinary bladder in two of the four preparations. In the trigonum, cytosolic and nuclear receptors could be measured in one and three preparations, respectively. The receptor concentrations in both trigonum and the bladder were lower than those in the urethra. By providing experimental evidence for the presence of estradiol receptors in the lower uninary tract, the present data advance the case for estradiol therapy in incontinent patients.

Adult↗
PubMed1981

Urokinase-like plasminogen activator as a marker of endometrial neoplasia.

Urokinase-like plasminogen activator (ULPA) was determined with a radioimmunoassay in uterine aspirates from 67 patients prior to curettage. In women with normal endometrial histology the mean concentration of ULPA was 5.4 +/- 2.5 microgram per liter. Only one of this aspirates contained more than 8 microgram per liter. In cases with malignant histology ULPA was above this level in 10 of 11 with a mean concentration of 31.2 +/- 31.4 microgram per liter. In cases with glandular cystic hyperplasia and/or adenomatous hyperplasia 6 of 15 had values above 8 microgram per liter with a mean of 10.3 +/- 6.1 microgram per liter. In addition to other parameters of malignancy such as cytology, detection of ULPA in uterine fluid might prove useful in screening examinations for endometrial neoplasia.

Female↗
PubMed1981

Effect of IUD on urokinase-like immunoreactivity and plasminogen activators in human uterine fluid.

The coexistence of two types of plasminogen activators in human uterine fluid was demonstrated. Immunologically, one was urokinase-like and the other tissue activator-like. Both activators contributed to the fibrinolytic activity of uterine fluid. Urokinase-like immunoreactivity decreased from the proliferative phase to the luteal phase and increased premenstrually. The increase in fibrinolytic activity premenstrually was ascribed mainly to an increase in concentration of the urokinase-like plasminogen activator. A much higher urokinase-like immunoreactivity was found in the uterine fluid from IUD-users than from non-users. The fibrinolytic activity of the uterine fluid, which in contrast to that of the endometrium is known to be depressed in IUD-users, was inhibited by an antiserum against the tissue activator but only weakly influenced by an antiserum against urokinase. This indicates the presence of urokinase inhibitors in the uterine fluid of IUD-users with a capacity to inhibit the enzymatic activity of the urokinase-like plasminogen activator.

Adolescent↗
PubMed1981

Fibrinolytic activity of human uterine fluid.

Normal biochemical composition of uterine fluid is essential for survival and successful implantation of the blastocyst. Fibrinolytic enzymes are involved in the implantation process. The variation of the fibrinolytic activity of uterine fluid with the phase of the normal menstrual cycle was investigated with the aid of radial enzyme diffusion on fibrin plates. The lytic activity increased during the proliferative phase, reached a maximum in midcycle and then fell to its lowest level during the luteal phase. It increased again in the premenstruum.

Body Fluids↗
PubMed1981

Fibrinolytic activity of uterine fluid in IUD-users.

The influence of an IUD on the fibrinolytic activity of uterine fluid was measured by radial enzyme diffusion on fibrin plates. In samples obtained from 48 women using Cu-IUDs, the fibrinolytic activity in mid-cycle phase was higher than that in the proliferative or the luteal phase. It increased in the premenstrual phase. Individual values before and after insertion of a Cu-T were determined in the proliferative-mid-cycle phase and the luteal phase in a group of 11 women. In contrast to earlier reports on the endometrium, the fibrinolytic activity of uterine fluid was lower after, than before, insertion of an IUD. This may be due to a lower concentration of activators and/or to an increased concentration of inhibitors of plasminogen activation.

Body Fluids↗
PubMed1981

Gigantomastia.

A case of gigantomastia is reported. The breast enlargement started some years before first pregnancy, during which a tremendous acceleration was observed. Various laboratory investigations elicited normal values, except for some high values of prolactin. No treatment was given. After delivery, the patient nursed her baby. Thirteen weeks later, lactation was inhibited with bromocriptine. A further 3 weeks later, a reduction mammoplasty was performed. One year later, the prolactin values were still normal. The etiology of this disease is not known. One possible explanation is an increased number or increased sensitivity of prolactin receptors in the target organ.

Adult↗
PubMed1980

Effect of tranexamic acid on progress of experimental tumours and on DNA-synthesis.

The fibrinolytic inhibitor tranexamic acid affects certain experimental tumours; it prolongs survival (Lewis lung adenocarcinoma), decreases tumour weight (C3H breast carcinoma), and inhibits ascitic production (AH 130 rat hepatoma). However, it does not significantly influence DNA-synthesis in cell suspensions prepared from the same tumours.

Adenocarcinoma↗
PubMed1980

Incidence of thrombosis after gynecologic surgery evaluated by an improved 125I-fibrinogen uptake test.

Two hundred and seventy-six women over 50 years of age who had gynecologic surgery were followed postoperatively with the 125I-fibrinogen uptake test (125I-FUT) to detect deep venous thrombosis (DVT). By using a newly devised technique based on conventional 125I-FUT, the exact depth of thrombi could be determined. The correlation between the results with our new technique and those from phlebography was almost 100%. Forty-seven (17%) of the women developed DVT postoperatively, 60% of them occurring in the calf muscle veins. Patients subjected to abdominal hysterectomies for malignant diseases had the highest incidence of DVT (36%), whereas those operated on for benign diseases with the same technique had the lowest (11%). Patients operated on for uterine prolapse had an intermediate DVT frequency of 15% but advanced age and estrogens given preoperatively increased the frequency. Neither the type of anesthesia (epidural or general), nor the duration of the operation, significantly influence the incidence of DVT in this study.

Age Factors↗
PubMed1980

A method for determination of the depth of thrombi after injection of fibrinogen labelled with iodine 125.

By using a specific characteristic of 125I decay, the depth of thrombi located in the lower limb could be determined. Twelve thrombi in nine patients were studied with the method and their localization was in all cases confirmed by phlebography and clinical examination. The results of the phlebograms were in good agreement with those of the 125I-fibrinogen method. The present method offers a promising alternative to phlebography in the diagnosis of postoperative thrombosis.

Aged↗
PubMed1980

Abruptio placentae--treatment with the fibrinolytic inhibitor tranexamic acid.

Abruptio placentae is known to have a bad prognosis for the fetus. Pathologic proteolysis, e.g. a pathologic activation of the coagulation mechanism and/or the fibrinolytic system is known to be a common complication in such cases. Analysis of the coagulation factors and components of the fibronlytic system in the acute stage of 14 cases confirmed the earlier finding of mainly an activation of the fibrinolytic system, which argues for the use of a specific inhibitor. 73 consecutive cases of abruptio placentae were treated with tranexamic acid in the acute stage. 67 of the patients were immediately delivered by cesarean section. The remaining six patients were in early gestational age and were treated for a prolonged period. The perinatal mortality of the entire group was only 8 per cent and the maternal mortality nil. None of the cases were complicated by hemorrhagic diathesis or thromboses. We believe that routine immediate treatment with tranexamic acid can reduce the perinatal mortality in cases of abruptio placentae.

Abruptio Placentae↗
PubMed1980

Effect of dihydroergotamine on coagulation factors and components of the fibrinolytic system.

Dihydroergotamine (DHE) was given to 20 gynecological patients, 0.5 mg i.m. preoperatively and again every twelfth hour for 7 days. No cases of thrombosis occurred as judged by the 125I-fibrinogen test. Increased concentrations, but within normal range, were found for fibrinogen factor VIII activity and related antigen, plasminogen and antithrombin III and decreased concentration for alpha 2-macroglobulin. The fibrinolytic activity of the wall of a superficial hand vein was not suppressed, which otherwise occurs postoperatively. Together with the increased concentration of antithrombin III this might explain the potentiating effect of DHE or prophylactic treatment with low-dose heparin.

Aged↗