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Biomedical subjects

B Anderberg

Publications and source records attributed to B Anderberg.

71 records · Page 4Linked to original sources
PubMed1981

Protein composition in single follicles, homogenates and fine-needle aspiration biopsies from normal and diseased human thyroid.

The protein composition of the thyroid colloid was analysed by microgel electrophoresis and densitometry in 41 euthyroid patients. The colloid samples were obtained from single follicles by micropuncture, from homogenates of microbiopsies or from aspiration biopsies. Fourteen of the patients had morphologically normal thyroid tissue, 18 had atoxic nodular goitre and 19 of the patients had atoxic adenoma. Ten of the patients with nodular goitre had prior to the investigation received lithium therapy for psychiatric disorders. The main component of the thyroid colloid was 19S thyroglobulin (TG), but larger iodoproteins (S-TG) and smaller protein fractions, an albumin-like protein and a pre-albumin fraction, were also present in varying relative amounts. Analyses of homogenates of microbiopsies from normal thyroid tissue demonstrated the same protein composition as observed in single follicles. In colloid samples from atoxic nodular goitre the lighter protein fractions were absent in most of the samples. Analyses of homogenates or aspiration biopsies could not demonstrate this alteration in the protein composition in nodular goitre. Lithium therapy resulted in a significantly lower amount of the lighter protein fractions but unchanged amounts of the globulin fractions in atoxic nodular goitre.

Adenoma↗
PubMed1981

Human thyroid blood flow response to endogenous, exogenous human, and bovine thyrotrophin measured by electromagnetic flowmetry.

Human thyroid blood flow (TBF) was studied with electromagnetic flowmetry during operation. Measurements were made of the effect on TBF of injections of bovine TSH into one inferior thyroid artery in 6 patients; 6 other patients were given human TSH, and in 10 patients measurements were made both of TBF and endogenous TSH released after administration of TRH in a peripheral vein. The TBF increased after all three types of injection. The mean of the TBF maxima after bovine TSH was 2.26 +/- 0.35 (mean +/- SEM) relative to basal TBF, after human TSH 1.97 +/- 0.28, and after TRH 1.64 +/- 0.20. In the three groups combined it was 1.92 +/- 0.16. The TBF was often increased already during the first recording period 1-10 min after TSH or TRH administration. The mean TBF was approximately doubled at 30-50 min. There were considerable inter-individual variations in the latent time and maximum response of TBF, especially after human TSH, but we found no correlation between the response and the TSH serum concentration in any group. A prompt, but inter-individually varying, increase in TBF was confirmed. This increase is suggested to be secondary to an increased intrafollicular metabolic activity and not primarily regulating thyroid function.

Adult↗
PubMed1981

Complications to subtotal parathyroidectomy.

Seventy-three patients were operated on for confirmed hyperparathyroidism. Identification of all glands was done by 1/3 biopsies for histopathologic examination on frozen section during the operation. In cases of adenoma only, the adenoma was removed except for the biopsies. In cases of hyperplasia, subtotal resection was made, leaving between a half-gland and a whole gland. The frequency of complications was analysed. Temporary hypocalcemia occurred in a similar frequency in both adenoma and hyperplasia groups. Permanent hypocalcemia was present in only one patient. At follow-up 3-5 years after operation, 98.6% of the patients were normocalcemic. Ionized and total serum calcium were normalized 3 months after operation and remained so for the rest of the follow-up period. Serum phosphate, serum magnesium and serum parathormone also remained normal. Thus identification of parathyroid glands with biopsies or subtotal parathyroidectomy in cases with hyperplasia did not increase the risk of postoperative complications.

Adult↗
PubMed1980

Cortisol, growth hormone and prolactin responses to insulin-induced hypoglycaemia in hyperthyroid patients before and during beta-adrenoceptor blockade.

Two beta-adrenoceptor blocking agents, metoprolol (beta-1-selective) and propranolol (non-selective), were used in the treatment of hyperthyroid patients. The response of pituitary stress hormones to insulin-induced hypoglycaemia was investigated before and during beta-adrenoceptor blockage. Treatment with metoprolol (n=10) or propranolol (n=10) produced no changes in the cortisol or prolactin responses to hypoglycaemia. Moreover the growth hormone response remained unaltered during treatment with metoprolol. A small, but statistically significant, augmentation of growth hormone response was obtained during treatment with propranolol. When twelve subjects, euthyroid after final conventional treatment (surger), radioiodine or thyrostatic drugs), were re-examined, the cortisol and prolactin responses were unchanged, although growth hormone concentrations reached a slightly higher maximum value (P less than 0.01). It was concluded that treatment with beta-blocking agents in hyperthyroid subjects has no clinically important influence on the release of pituitary stress hormones during hypoglycaemia.

Adrenergic beta-Antagonists↗
PubMed1980

Protein composition of the thyroid colloid in patients with hyperthyroidism.

Analysis of the protein composition of the thyroid colloid was performed in 28 patients operated on for hyperthyroidism. Fifteen of the patients were treated before the operation with carbimazole combined with thyroxine and 13 were treated with propranolol alone. Colloid was collected by micropuncture of single follicles in peroperative thyroid biopsies. The protein composition was analysed by microgel electrophoresis and densitometry, both in the colloid samples and in the supernatant fraction of homogenates of microbiopsies from the thyroid specimens. The analyses showed that, during treatment with carbimazole and thyroxine, the relative amount of the larger thyroglobulin aggregates (S-TG) was decreased compared with the relative amount observed in the colloid from normal thyroid tissue. In the hyperfunctioning thyroid tissue from the propranolol-treated patients the protein composition of the colloid was similar to that observed in normal tissue and the relative amount of the S-TG fractions was significantly higher than in the carbimazole- and thryoxine-treated group. It may be concluded that the increased release of thyroid hormones in hyperthyroidism is not combined with changes in the protein composition of the thryoid colloid. The decreased relative amount of the S-TG fractions in the thyroid colloid from patients treated with carbomazole and thryoxine was probably due to an insufficient capacity to iodinate thyroglobulin.

Carbimazole↗
PubMed1980

Protein synthesis in hyperfunctioning human thyroid tissue.

The rate of protein synthesis was examined in vitro in thyroid tissue slices. The material was obtained from euthyroid patients operated on for hyperparathyroidism and from hyperthyroid patients with Graves' disease pre-operatively treated with propranolol alone. The rate of protein synthesis was calculated from the incorporation of [3H]leucine radioactivity in the soluble thyroid proteins (mainly the colloid proteins), the amount of soluble protein per mg tissue and amount of DNA. Based on these figures it was observed that the rate of protein synthesis was 3.2 times higher in the hyperfunctioning thyroid tissue than in the normal tissue. A significant correlation was observed between this activity factor and the serum concentrating of thyroxine. It is concluded that the increased hormone formation in hyperthyroid Graves' disease at least partly depends on an increased rate of protein synthesis in the follicular epithelium. This could be due to stimulation by thyroid immunoglobulins, but other pathogenetic mechanisms may be responsible.

Humans↗
PubMed1979

Depletion of colloid 27S and larger thyroid iodoproteins following treatment with propylthiouracil.

By means of a micropuncture technique and microgel electrophoresis the luminal content of single rat thyroid follicles were analyzed during PTU treatment for 4 weeks. The relative amount of aggregates of thyroglobulin (i.e. the 27S iodoprotein and larger complex) was reduced from 15 per cent to approximately 3 per cent after 48 h. The aggregates were practically absent after 2 weeks. The thyroglobulin fraction was the predominant colloid constituent. The protein concentration of the colloid was not changed at the end of the period of PTU treatment (8.9 g/100 ml). It is concluded that PTU inhibits the aggregation of thyroglobulin to a larger complex. The results further indicate that the aggregates of thyroglobulin are preferentially localized to the periphery of the lumen in the larger follicles.

Animals↗
PubMed1979

Peroperative estimation of the size of the thyroid remnant.

A new method for estimation of the size of the remaining tissue after thyroid resection is described. The method has been tested on cadavers and during lobectomy in a series of patients. It is shown that intraoperative quantification of the thyroid remnant can be performed mostly with +/- 0.5 g for an individual thyroid lobe with a coefficient of variation of +/- 7.6%. In patients with toxic nodular goiter and calcifications, however, the error might be larger due to the varying homogeneity of the thyroid tissue. In 73 patients with negative antibody titers operated on for hyperthyroidism it was shown that the size of the thyroid remnant was negatively correlated to the delta TSH, determined 6 weeks after the operation.

Cadaver↗
PubMed1978

Change of the protein composition of the thyroid colloid during treatment with propylthiouracil and thyroxine: a microgel electrophoretic study of single rat thyroid follicles.

Prophylthiouracil and thyroxine were given daily to rats for 4 weeks. Samples of colloid were collected in vivo from the superficial thyroid follicles during this period and their protein composition was analysed by gel electrophoresis. It was observed that the aggregates of thyroglobulin, i.e., the 27-S thyroid iodoprotein and the heavier fractions, were reduced to 50% after 1 week and were almost absent after 2 weeks. A faster migrating thyroglobulin fraction was observed in the samples of colloid and in the homogenate of the whole gland after 48 h of treatment. During the following period of treatment there was an increase in the relative amount of the faster migrating thyroglobulin fraction compared to 19-S thyroglobulin in the colloid, the former comprising approx. 75% of the globulins after 4 weeks, It can be concluded that propylthiouracil inhibits the formation of the 27-S iodoprotein and that a structurally altered and iodine-poor thyroglobulin fraction is accumulated in the follicle lumen.

Animals↗
PubMed1978

B- and T-lymphocytes in toxic diffuse goitre.

Operation specimens of thyroid tissue from patients with Graves' disease were digested, with collagenase, down to a cell suspension. The lymphocytes in the suspension were concentrated by density-gradient sedimentation and the proportions of B and T lymphocytes were determined. Six patients with Graves' disease were studied. The proportions of B and T lymphocytes in the thyroid glands were similar to the proportions of B and T lymphocytes in the venous blood from healthy subjects.

Adult↗
PubMed1978

Clinical experience of parathyroid autotransplantation.

Parathyroid tissue was transplanted from the neck to the muscles of the non-dominant forearm in 18 patients. The difference in concentration of parathormone between the transplant arm and the contralateral arm was used as a test of parathyroid function. All patients showed a difference in concentration between the arms. Three patients needed calcium supplement for 2 months after surgery, and one patient with no parathyroid tissue left in situ also needed vitamin D for 8 months. The results indicate that parathyroid tissue transplanted to the arm continues to function, and is able to keep the patient normocalcaemic even when no parathyroid tissue is left in situ.

Adenoma↗
PubMed1977

A microgel electrophoretic analysis the colloid in single rat thyroid follicles during thyroxine suppression.

A sequential study of the effect of thyroxine (T4) on the protein composition of the colloid in the rat thyroid gland has been carried out in 24 rats. Daily ip injections of 20 mug thyroxine were given and the colloid was analyzed after 1, 2, 3 and 4 days and 1, 2 and 3 weeks. The protein constituents were separated by microgel electrophoresis and measured by microdensitometry. The incorporation of [3H]leucine into the thyroglobulin fraction and into the albumin-like protein in the homogenate of the gland was analyzed after T4 suppression for 2 days. The 2 h thyroidal iodine uptake was analyzed during the first week of T4 administration. During the second and the third day of T4 administration, the amount of 27S and larger complexes of thyroglobulin was reduced by 30-40 per cent and the amount of the albumin-like protein was significantly increased and present in almost all samples. Preliminary data indicated that during the same period the relative amount of [3H]leucine activity was increased in the albumin-like protein fraction compared to the thyroglobulin fraction in the thyroid. It is concluded that the exocytotic vesicles contain the albumin-like protein. The results further indicate that the administration of T4 temporarily changes the relative rates of formation of thyroglobulin and of the albumin-like protein in the follicle.

Animals↗
PubMedDate not supplied

The nude mouse. A possible experimental model for investigation of human thyroid tissue.

The effect of transplanting hyperfunctioning human thyroid tissue to athymic, nude mice was explored. The thyroid tissue was obtained from a patient with diffuse, thyrotoxic goiter preoperatively treated with a beta-adrenoreceptor blocker. One transplant was placed in each groin of 4 nude mice. Light microscopy after 4 weeks showed that transplants consisted mainly of typical follicles; capillaries were common at the periphery of the transplant but not in between the follicles. The ultrastructure of the follicle cells in the transplants was similar to that of follicle cells in general. All 8 transplants accumulated 125I as revealed by external counting; the uptake in each transplant 24 h after administration of radioiodine was 4-25% of that in the thyroid of the transplanted mouse. The release of radioiodine, measured during a period of 14 days, from the thyroid of a transplanted mouse was delayed as compared to a nontransplanted control; this indicates that the mouse thyroid activity was suppressed due to hormone production in the transplants. Electron microscopic autoradiography 24 h after injection of 125I showed that protein-bound label in transplants was located mainly in follicle lumens. The radioiodine was incorporated in thyroglobulin as demonstrated by separation of soluble proteins from transplants by sucrose density gradient centrifugation. The present study shows that human thyroid tissue can be transplanted to nude mice with maintained structural and functional properties. Transplantation of human thyroid tissue might be a useful model for studies of thyroid diseases.

Animals↗
PubMedDate not supplied

Thyroid blood flow rate in man. Electromagnetic flowmetry during operation in euthyroid normal gland, nontoxic goiter, and hyperthyroidism.

Human thyroid blood flow rate (TBF) was measured during operations by electromagnetic flowmetry in 75 euthyroid patients with normal thyroid tissue, nodular goiter, or solitary adenoma, and in 22 hyperthyroid patients with diffuse or nodular goiter. Blood flow rate was measured in one to four of the thyroid arteries. No difference in blood flow rate was seen between the left and right lobes. The slight difference found between the inferior and superior arteries was not significant. In each subject, total TBF was calculated as 4 times the mean of the recorded blood flow in the single arteries. The total TBF was 31 (9-109) ml/min (inner 95 percentile range) in euthyroid patients, similar in all 3 groups. This is less than in most earlier reports. The relative TBF was 1.2 (0.4-3.8) ml/min/g thyroid tissue in normal thyroid tissue and 0.6 (0.1-3.7) ml/min/g in nontoxic nodular goiter (p less than 0.01). Patients with hyperthyroidism had a higher total TBF 54 (15-197) ml/Min (p less than 0.001), despite preoperative treatment giving euthyroidism, Similar TBF rates were found in 3 hyperthyroid patients given propranolol preoperatively. Electromagnetic flowmetry is applicable to study thyroid blood flow rate. Human TBF shows considerable interindividual variations, which must be kept in mind when studying directly the rate of thyroid hormone secretion from arteriovenous gradients.

Adult↗
PubMedDate not supplied

Bacteremia following diagnostic and therapeutic ERCP.

The occurrence of bacteremia in association with diagnostic or therapeutic ERCP was studied in 180 patients undergoing a total of 194 examinations. Nineteen (15%) of 126 diagnostic procedures and 18 (27%) of 68 therapeutic procedures were associated with bacteremia (p less than 0.1). Nine patients had polymicrobial bacteremia and a total of 16 species were detected. Different streptococci, mainly alpha-hemolytic, were the most common bacteria which were identified in 38% of the bacteremic patients. There were no significant differences with regard to the occurrence of fever, pancreatitis, or septic complications between the diagnostic and therapeutic groups of patients. Neither did the complication rate in patients with bacteremia differ from that in patients without bacteremia, whether the procedure was diagnostic or therapeutic. Complication rates did not differ between patients with and patients without pancreaticobiliary obstruction. However, the majority of patients with biliary stasis had drainage with relief of the obstruction at the time of the diagnostic ERCP. We conclude that general routine antibiotic prophylaxis is not indicated in patients undergoing diagnostic or therapeutic ERCP. The question whether such prophylaxis should be given with certain diagnoses or treatments, or in patients with valvular heart disease, remains to be answered in controlled randomized studies.

Aged↗