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Biomedical subjects

B A Howard

Publications and source records attributed to B A Howard.

26 records · Page 2Linked to original sources
PubMedDate not supplied

Fragmentation of the ribosome to investigate RNA-ligand interactions.

RNA molecules perform a variety of important and diverse functions and, therefore, an understanding of their structure and interaction with proteins and ligands is essential. Large RNA molecules (for example, the ribosomal RNAs) are complex and hence reports describing their fragmentation into functional subdomains has provided a means for their detailed analysis. We present here an in vivo approach to study RNA-ligand interactions. This is based on the concept that an RNA fragment could mimic a drug-binding site present on the intact molecule. Overexpression of the fragment would sequester the drug thereby permitting the continued functioning of the ribosome and, thus, ensuring cell viability. Accordingly, a fragment of 16S rRNA encompassing the spectinomycin-binding domain in helix 34 (nucleotides 1046-1065 and 1191-1211) was cloned and in vivo expression resulted in drug resistance. Furthermore, an RNA fragment lacking flanking sequences to helix 34 was also selected from among a pool of random rRNA fragments and shown to confer spectinomycin resistance. A similar in vitro approach is also described for the analysis of rRNA molecules that interact with the yeast elongation factor 3 (EF-3).

Anti-Bacterial Agents↗
PubMedDate not supplied

Diagnostic imaging of trauma to the ankle and foot. Part VI: Forefoot injuries.

Drs. Mitchell et al. have provided a valuable radiologic perspective of trauma to the ankle and foot. Previous articles in this series consisted of analyzing ankle fractures, ligamentous injuries, trauma to the talus, calcaneus, and midfoot. Plain x-ray films and sophisticated studies will assist in recognizing conditions described in this series of papers. The following represents the final part of this discussion.

Ankle↗
PubMedDate not supplied

Diagnostic imaging of trauma to the ankle and foot: I. Fractures about the ankle.

Drs. Mitchell et al. provide a valuable radiologic perspective of trauma to the ankle. The review is divided into six parts. The initial presentation deals with ankle fractures. Subsequent dialogues will consist of ligamentous injuries, trauma to the talus, calcaneal fractures, midfoot, and forefoot injuries. Plain x-ray film, as well as sophisticated studies, will assist in recognizing these conditions.

Ankle Injuries↗
PubMedDate not supplied

Diagnostic imaging of trauma to the ankle and foot: IV. Fractures of the calcaneus.

Drs. Mitchell et al. provide a valuable radiologic perspective of trauma to the ankle and foot. This review is divided into six parts. The previous presentations involved ankle fractures, ligamentous injuries, and trauma to the talus. Subsequent dialogues will consist of midfoot and forefoot injuries. Plain x-ray films, as well as sophisticated studies, will assist in recognizing these conditions.

Calcaneus↗