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Biomedical subjects

Arif Hussain

Publications and source records attributed to Arif Hussain.

24 records · Page 2Linked to original sources
PubMed2004

Prevalence of non-vision-impairing conditions in a village in Chakwal district, Punjab, Pakistan.

PURPOSE: To determine the prevalence of non-vision-impairing ocular conditions (NVIC) and estimate the number of primary eye care treatments per 1000 population per month. METHODS: A cross-sectional study in a random sample of 1670 people was done to determine the load of NVIC in a village in Chakwal district. RESULTS: The prevalence of NVIC was 30.6% (306 per 1000 population). NVIC with the exclusion of presbyopia accounted for 14.6%. The main NVIC were allergic conjunctivitis (3.7%), bacterial conjunctivitis (3.5%), pterygium/pinguicula (2.6%) and acute/chronic dacryocystitis (1%). The average Complaint Frequency (CF) per month/1000 population was 55, excluding complaints of near vision and watery eyes. CONCLUSIONS: The foundation of a comprehensive district eye care strategy in the light of VISION 2020 - the Right to Sight - remains an effective primary eye care service whose elements are treatment of NVIC, detection and referral of cataracts and refractive errors, and promotion of eye health.

Adolescent↗
PubMed2003

Cataract blindness in Chakwal District, Pakistan: results of a survey.

AIM: To present the results of a rapid assessment of cataract surgical services in Chakwal District, Pakistan. METHODS: 40 clusters of 40 persons of 50 years and older (1600 eligible persons) were selected by systematic random sampling from the entire Chakwal district. A total of 1505 persons were examined (coverage 94%). RESULTS: Cataract is the major cause of bilateral blindness (46.5%). The age and sex adjusted prevalence of bilateral cataract blindness (VA < 3/60) in people of 50 years and older was 2.0% (95% CI: 1.2-3.2%), with a cataract surgical coverage of 92% for males and 73% for females, a significant difference. The prevalence of bilateral cataract and VA < 6/60 in persons of 50 years and older was 5.1% (95% CI: 3.6-6.9), an estimated total of 8833 persons. In this last group, the surgical coverage was 66% (persons) and 50% (eyes). Of the patients operated with IOL implantation, 12% could not see 6/60, while 36% of those operated without IOL could not see 6/60. 'No services' (18%) and 'cannot afford' (18%) were mentioned most as reasons why surgery had not been done, followed by 'no information' (13%), 'waiting for maturity' (12%) and 'old age, no need for surgery' (12%). CONCLUSION: The number of cataract operations in Chakwal District can be increased by reducing the threshold for cataract surgery to VA < 6/60 or less. A special approach to operate more females may be required. Results from this survey can be used for a planning exercise to optimize eye care services in the district. Sustained efforts have to be made to increase the number of IOL surgeries, through making IOL surgery available locally at an affordable cost.

Aged↗
PubMed2002

Endoplasmic reticulum calcium pool depletion-induced apoptosis is coupled with activation of the death receptor 5 pathway.

Thapsigargin (TG), by inducing perturbations in cellular Ca(2+) homeostasis, has been shown to induce apoptosis. The molecular mechanisms of Ca(2+) perturbation-induced apoptosis are not fully understood. In this study, we demonstrate for the first time that TG-mediated perturbations in Ca(2+) homeostasis are coupled with activation of the death receptor 5 (DR5)-dependent apoptotic pathway in human cancer cells. TG selectively upregulated DR5 but had no effect on the expression of the other TRAIL receptor, DR4. TG also upregulated the expression of the DR5 ligand TRAIL (tumor necrosis factor-related apoptosis inducing ligand), albeit in a cell-type specific manner. TG-induced apoptosis has been shown to be associated with activation of the mitochondrial pathway. We found that TG upregulation of DR5 and TRAIL was coupled with caspase 8 activation and Bid cleavage, suggesting that the TG-regulated DR5 pathway could be linked to the mitochondrial pathway. TG enhanced not only DR5 mRNA stability but also increased induction of the DR5 genomic promoter-reporter gene. The TG-induced increase in DR5 expression appeared to occur as a consequence of TG-induced endoplasmic reticulum (ER) Ca(2+) pool depletion. Thus, we report our novel findings that ER Ca(2+) pool depletion-induced apoptotic signals are mediated, at least in part, via a DR5-dependent apoptotic pathway and there appears to be a cross-talk between the death receptor and mitochondrial pathways.

Apoptosis↗