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Anupam Basu

Publications and source records attributed to Anupam Basu.

6 recordsLinked to original sources
PubMed2005

Your diagnosis? Scaphoid fracture.

Fractures of the scaphoid are the most common carpal bone fracture. With careful management, prognosis is excellent. Imaging is critical, not only for early diagnosis, but also for monitoring patients after initial treatment to detect unstable fractures, the common complications of nonunion and avascular necrosis, and the need for surgical treatment.

Adult↗
PubMed2004

Coordinated problem solving through resource sharing in a distributed environment.

An important feature in a distributed problem solving system is that the resources of different nodes can be shared through cooperation. In this paper, the generalized partial global planning (GPGP) approach used for multiagent systems is extended by providing a coordination mechanism for resource sharing across nodes. In our framework, multiple conflicting criteria (or objectives) like quality, cost, and duration may be associated with an input task. Preference ratings expressed subjectively may be assigned to each of the criteria. Task assignment in this system, which is a multiobjective decision making problem, is important for the satisfaction of the criteria. It has to be done with imprecise information since the system is dynamic and preference ratings are specified subjectively. A technique for task assignment using the fuzzy set approach is also presented in this paper. Simulation studies for the coordination mechanism and the task assignment have been performed to demonstrate their effectiveness.

Letter↗
PubMed2003

Substitution of carbonate buffer by water for IgG immobilization in enzyme linked immunosorbent assay.

The first step of enzyme linked immunosorbent assay (ELISA), namely, adsorption of antigen or antibody to the plastic microtiter well plate, was studied as a function of insolubility of IgG in water. Immobilization efficiency was assessed in terms of number of wells coated per milliliter of primary antiserum. We have compared different coating/immobilization protocols, i.e., direct and indirect immobilization of primary antibody to the plastic microtiter well plate using carbonate buffer and phosphate buffer with glutaraldehyde. We have observed efficient coating when the immobilization of primary antibody through an immunobridge technique was performed, where water was used as a coating medium. It gave a higher number of wells coated per milliliter of anti-serum (primary or secondary) than other compared coating protocols and it allowed the use of serum (non-immune) and anti-serum (primary and secondary antibody) dilutions, avoiding the need for gamma-globulin purification from normal and immunized serum.

Adipates↗
PubMed2003

One step enzyme linked immunosorbent assay for direct estimation of serum testosterone.

One step competitive enzyme linked immunosorbent assay (ELISA) for direct estimation of testosterone in human serum is described. Testosterone-3-O-carboxymethyl-oxime-bovine serum albumin (testosterone-3-O-CMO-BSA), was used as immunogen and testosterone-3-O-carboxymethyl-oxime-adipic-acid dihydrazide-horseradish peroxidase (testosterone-3-O-CMO-ADH-HRP) was used as tracer. To the testosterone antibody coated microtiter wells, standard or serum samples (100 microL), along with testosterone-3-O-CMO-ADH-HRP conjugate (100 microL) were incubated for 1 h at 37 degrees C. Bound enzyme activity was measured by using tetra methyl benzidine/hydrogen peroxide (TMB/H2O2) as a substrate. In this new strategy, charcoal stripped pooled human serum spiked with non-cross reactive C18, C19, C21, and C27 steroids, used for preparing the standards and blocking the sex hormone binding globulin (SHBG)/and other steroid binding globulins (SBG). The sensitivity of the assay was 0.015 ng/mL. The intra-assay and inter-assay coefficients of variation (CVs) were ranged from 7.8 to 11.8 and 4.8 to 10.4, respectively. The serum testosterone values, obtained by this method, were correlated well with those obtained by radioimmunoassay r = .98 (n = 100).

Adipates↗