Biomedical subjects

Anita Singh

Publications and source records attributed to Anita Singh.

6 recordsLinked to original sources

PubMed2006

Dietary effects of universal-free school breakfast: findings from the evaluation of the school breakfast program pilot project.

OBJECTIVE: To determine the effects of offering universal-free school breakfast in elementary schools on students' dietary outcomes. DESIGN: Experimental study with random assignment of 153 matched elementary schools in six school districts. Treatment schools offered universal-free school breakfast, and control schools continued to operate the traditional means-tested School Breakfast Program. Twenty-four-hour dietary recalls were collected from sample students near the end of the first year. SUBJECTS: About 30 students in second through sixth grades were randomly selected from each school (n=4,358). INTERVENTION: Free school breakfasts were made available to all students in treatment schools, regardless of family income, for three consecutive school years (2000-2001 to 2002-2003). MAIN OUTCOME MEASURES: Breakfast consumption and food and nutrient intake. STATISTICAL ANALYSES: Hierarchical mixed-models and logistic regression, adjusting for age, sex, minority status, and income eligibility for the regular school meal programs, were used to estimate effects. RESULTS: Despite a significant increase in school breakfast participation among sample students in treatment schools (from 16% to 40%, P<0.01), the rate of breakfast skipping did not differ between groups (4% overall). Treatment school students were more likely to consume a nutritionally substantive breakfast (P<0.01), but dietary intakes over 24 hours were essentially the same. CONCLUSIONS: Making universal-free school breakfast available in elementary schools did not change students' dietary outcomes after nearly 1 year. To improve children's diets overall, efforts should focus on ensuring all students have access to a healthful breakfast, at home or at school.

Body Weight↗

PubMed2005

Mechanical properties of spinal nerve roots subjected to tension at different strain rates.

An understanding of the biomechanical and physiological properties of spinal nerve roots, particularly in response to tension, is critical in understanding the pathomechanisms of pain and nerve root injury and subsequent management of related injuries. Biomechanical properties of dorsal nerve roots at the lumbar and sacral levels were evaluated at various strain rates. Nerve roots were stretched at two different rates, 0.01 mm/s (Group A, quasistatic) and 15 mm/s (Group B, dynamic). Load, displacement and digital video data were obtained as the nerve roots were stretched until failure. Maximum stress, strain at maximum stress and modulus of elasticity (E) were calculated from the load-displacement measurements. Comparison of mechanical properties and failure patterns of nerve roots at two different rates revealed significant differences. Maximum load, maximum stress and E values of 5.7+/-2.7 gm, 257.9+/-111.3 kPa and 1.3+/-0.8 MPa were observed for Group A and 13.9+/-7.5 gm, 624.9+/-306.8 kPa and 2.9+/-1.5 MPa were observed for Group B, respectively. Higher maximum load, maximum stress and E values occurred at the dynamic stretch rate as compared to the quasistatic stretch rate, illustrating the strain-rate dependency of spinal nerve roots. No differences were observed in the strain values. Differences in mechanical behavior of nerve roots were also observed among the four root levels (L4-S1). A significant interaction effect was observed between nerve root diameter and stretch rates. Overall, results from the present study demonstrate viscoelastic material properties of spinal nerve roots and provide better insight on the tensile properties of nerve roots at different strain rates.

Animals↗

PubMed2004

Demonstration of substance P, calcitonin gene-related peptide, and protein gene product 9.5 containing nerve fibers in human cervical facet joint capsules.

STUDY DESIGN: Human cervical facet joint capsules were evaluated by immunohistochemistry. OBJECTIVES: To study the neuropeptide innervation of the cadaveric cervical facet joint capsules. SUMMARY OF BACKGROUND DATA: Various clinical and biomechanical studies indicate a role for cervical facet joint capsules in the etiology of neck pain. However, studies on innervation of these capsules are very limited. There is also a dearth of studies on the neuropeptide nature of this innervation. METHODS: Facet joint capsules harvested from unembalmed cadavers were studied by the avidin biotin peroxidase method for the presence of nerve fibers. Neuropeptide innervation was investigated by using antisera to substance P and calcitonin gene-related peptide. Antisera to protein gene product 9.5 (PGP 9.5), a general neuronal marker, were also used. RESULTS: In a study of 12 human cervical facet joint capsules, short segments of substance P were observed in 6 capsules, while fibers reactive to calcitonin gene-related peptide were demonstrated in 7 capsules. Nerve fibers immunoreactive to protein gene product 9.5 were also observed in 9 of the 14 capsules studied. Protein gene product 9.5 reactive fibers were the most extensively distributed fibers, observed as bundles and also as single fibers. CONCLUSIONS: An abundance of protein gene product 9.5 reactive nerve fibers indicates an extensive innervation of the cervical facet joint capsules. The presence of substance P and calcitonin gene-related peptide reactive nerve fibers in a population of these lends credence to cervical facet joint capsules as a key source of neck pain.

Aged↗

PubMed2003

Are women with urogenital atrophy symptomatic?

OBJECTIVE: The purpose of this study was to determine the degree of correlation between physical signs of genital atrophy and symptoms that are suggestive of atrophic vaginitis. STUDY DESIGN: Female volunteers (n = 135; mean age, 69 years) rated the presence and severity (rating, 0-3) of vaginal atrophy symptoms. The presence and severity of vaginal mucosal changes, which included vaginal pH (0-3), were recorded during a pelvic examination. A vaginal cytologic maturation value was performed. Symptoms, signs, pH, and maturation value were correlated by the Spearman rank test. RESULTS: Symptom scores were low (mean, 0.41; range, 0-2.6). Symptoms were only weakly correlated with physical findings (r = 0.14) and not with maturation value (r = 0.06) or age (r = -0.004). There was a moderate correlation between physical examination score and maturation value (r = -0.48). In women > or =65 years old, symptom score and physical examination score were correlated weakly (r = 0.25). Low pH correlated well with high maturation value (r = -0.52). Women who were undergoing estrogen therapy had higher symptoms scores (P =.0007) and maturation values (P =.0002) than women who were not undergoing therapy. CONCLUSION: Although urogenital atrophy occurs universally after menopause, most elderly women are minimally symptomatic. Those women on estrogen replacement therapy may be more symptomatic. Symptoms alone should not be used as a guide for the initiation of estrogen therapy.

Adult↗

PubMed2003

Design and synthesis of semicarbazones and their bio-isosteric analogues as potent anticonvulsants: the role of hydrogen bonding.

A series of p-nitrophenyl substituted semicarbazones (4a-c) and phenoxy/p-bromophenoxy acetyl hydrazones (8a-q) were synthesized and their anticonvulsant activity was screened against maximal electroshock seizure (MES), subcutaneous metrazole (ScMet) and subcutaneous strychnine (ScSty) tests. Compounds 4a-c with -NHCO- were found to be the most active in all these tests. These compounds were also active in the MES test after oral administration in rats. On the other hand, compounds 8a-q with -OCH2- were devoid of anticonvulsant activity. The studies revealed that the hydrogen bonding domain in semicarbazones, adjacent to the lipophilic aryl ring, is essential for the anticonvulsant activity.

Administration, Oral↗

PubMed2002

Choline ingestion does not modify physical or cognitive performance.

OBJECTIVES: This study was undertaken to determine whether choline ingestion improves physical and cognitive performance following exhaustive load carriage exercise. METHODS: In a double-blind crossover study, 13 men (28 +/- 2 years) underwent four test sessions: load carriage treadmill and no-load carriage test sessions after taking choline or placebo. Physical and cognitive performance batteries were administered at the end of the test sessions. RESULTS: Choline ingestion (50 mg/kg body weight) significantly (p < 0.05) increased plasma choline concentrations during the load and no-load carriage test sessions. However, plasma choline did not decrease during the placebo load carriage test session, an indication that load carriage does not deplete circulating choline. There were no differences in performance on physical tasks, and choline ingestion had no effect on reaction time, logical reasoning, vigilance, spatial memory, or working memory. CONCLUSION: In healthy men, supplemental choline did not affect physical or cognitive performance after exhaustive physical activity.

Adult↗