Biomedical subjects

Angelo Sassaroli

Publications and source records attributed to Angelo Sassaroli.

8 recordsLinked to original sources

PubMed2005

Three-element phased-array approach to diffuse optical imaging based on postprocessing of continuous-wave data.

We present a multielement phased-array approach to diffuse optical imaging based on postprocessing of continuous-wave data for the improvement of spatial resolution. In particular, we present a theoretical and experimental analysis of the performance of a three-element source array in the study of an optically turbid medium with two embedded cylindrical inclusions. We find that the proposed phased-array approach is able to resolve two cylinders with side-to-side separation of 10 mm that are not resolved by the intensity associated with a single light source.

Image Enhancement↗

PubMed2005

Spatial and spectral information in optical mammography.

This article reviews our research activities in the area of optical mammography and relates them to the historical developments and the current state and trends in the field. The guiding threads for this article are the roles played in optical mammography by spatial and spectral information. The first feature, spatial information, is limited by the diffusive nature of light propagation but can take advantage of the exceptionally high optical contrast featured by blood vessels and blood-rich areas in the breast. We describe a method to correct for edge effects, a spatial second-derivative algorithm, and a two-dimensional phased-array approach that enhance the image contrast, the spatial resolution, and the depth discrimination in optical mammograms. The second feature, spectral information, is the most powerful and unique capability of optical mammography, and allows for functional measurements associated with hemoglobin concentration and oxygenation, water concentration, lipids content, and the wavelength dependence of tissue scattering. We present oxygenation-index images obtained from multi-wavelength optical data that point to the diagnostic potential of oxygenation information in optical mammography. The optimization of the spatial and spectral information in optical mammography has the potential to create a role for this imaging modality in the detection and monitoring of breast cancer.

Breast Neoplasms↗

PubMed2004

Comment on the modified Beer-Lambert law for scattering media.

We present a concise overview of the modified Beer-Lambert law, which has been extensively used in the literature of near-infrared spectroscopy (NIRS) of scattering media. In particular, we discuss one form of the modified Beer-Lambert law that is commonly found in the literature and that is not strictly correct. However, this incorrect form of the modified Beer-Lambert law still leads to the correct expression for the changes in the continuous wave optical signal associated with changes in the absorption coefficient of the investigated medium. Here we propose a notation for the modified Beer-Lambert law that keeps the typical form commonly found in the literature without introducing any incorrect assumptions.

Biophysics↗

PubMed2004

Optical measurements of absorption changes in two-layered diffusive media.

We have used Monte Carlo simulations for a two-layered diffusive medium to investigate the effect of a superficial layer on the measurement of absorption variations from optical diffuse reflectance data processed by using: (a) a multidistance, frequency-domain method based on diffusion theory for a semi-infinite homogeneous medium; (b) a differential-pathlength-factor method based on a modified Lambert-Beer law for a homogeneous medium and (c) a two-distance, partial-pathlength method based on a modified Lambert-Beer law for a two-layered medium. Methods (a) and (b) lead to a single value for the absorption variation, whereas method (c) yields absorption variations for each layer. In the simulations, the optical coefficients of the medium were representative of those of biological tissue in the near-infrared. The thickness of the first layer was in the range 0.3-1.4 cm, and the source-detector distances were in the range 1-5 cm, which is typical of near-infrared diffuse reflectance measurements in tissue. The simulations have shown that (1) method (a) is mostly sensitive to absorption changes in the underlying layer, provided that the thickness of the superficial layer is approximately 0.6 cm or less; (2) method (b) is significantly affected by absorption changes in the superficial layer and (3) method (c) yields the absorption changes for both layers with a relatively good accuracy of approximately 4% for the superficial layer and approximately 10% for the underlying layer (provided that the absorption changes are less than 20-30% of the baseline value). We have applied all three methods of data analysis to near-infrared data collected on the forehead of a human subject during electroconvulsive therapy. Our results suggest that the multidistance method (a) and the two-distance partial-pathlength method (c) may better decouple the contributions to the optical signals that originate in deeper tissue (brain) from those that originate in more superficial tissue layers.

Absorption↗

PubMed2003

Solution of the time-dependent diffusion equation for layered diffusive media by the eigenfunction method.

An exact solution of the time-dependent diffusion equation for the case of a two- and a three-layered finite diffusive medium is proposed. The method is based on the decomposition of the fluence rate in a series of eigenfunctions and upon the solution of the consequent transcendental equation for the eigenvalues obtained from the boundary conditions. Comparisons among the solution of the diffusion equation and the results of Monte Carlo simulations show the correctness of the proposed model.

Journal Article↗

PubMed2002

Maps of optical differential pathlength factor of human adult forehead, somatosensory motor and occipital regions at multi-wavelengths in NIR.

The optical differential pathlength factor (DPF) is an important parameter for physiological measurement using near infrared spectroscopy, but for the human adult head it has been available only for the forehead. Here we report measured DPF results for the forehead, somatosensory motor and occipital regions from measurements on 11 adult volunteers using a time-resolved optical imaging system. The optode separation was about 30 mm and the wavelengths used were 759 nm, 799 nm and 834 nm. Measured DPFs were 7.25 for the central forehead and 6.25 for the temple region at 799 nm. For the central somatosensory and occipital areas (10 mm above the inion), DPFs at 799 nm are 7.5 and 8.75, respectively. Less than 10% decreases of DPF for all these regions were observed when the wavelength increased from 759 nm to 834 nm. To compare these DPF maps with the anatomical structure of the head, a Monte Carlo simulation was carried out to calculate DPF for these regions by using a two-layered semi-infinite model and assuming the thickness of the upper layer to be the sum of the thicknesses of scalp and skull, which was measured from MRI images of a subject's head. The DPF data will be useful for quantitative monitoring of the haemodynamic changes occurring in adult heads.

Brain↗

PubMed2002

Analytical approximate solutions of the time-domain diffusion equation in layered slabs.

Time-domain analytical solutions of the diffusion equation for photon migration through highly scattering two- and three-layered slabs have been obtained. The effect of the refractive-index mismatch with the external medium is taken into account, and approximate boundary conditions at the interface between the diffusive layers have been considered. A Monte Carlo code for photon migration through a layered slab has also been developed. Comparisons with the results of Monte Carlo simulations showed that the analytical solutions correctly describe the mean path length followed by photons inside each diffusive layer and the shape of the temporal profile of received photons, while discrepancies are observed for the continuous-wave reflectance or transmittance.

Diagnostic Imaging↗

PubMedDate not supplied

Two-dimensional phased arrays of sources and detectors for depth discrimination in diffuse optical imaging.

We present a multisource, multidetector phased-array approach to diffuse optical imaging that is based on postprocessing continuous-wave data. We previously showed that this approach enhances the spatial resolution of diffuse optical imaging. We now demonstrate the depth discrimination capabilities of this approach and its potential to perform tomographic sectioning of turbid media. The depth discrimination results from the dependence of the sensitivity function on the depth coordinate z. To demonstrate the potential of this approach, we perform an experimental study of a turbid medium containing cylindrical inhomogeneities that are placed 2.0, 3.0, and 4.0 cm from a seven-element, 2-D source array. A single detector element is placed at a distance of 6.0 cm from the source array, and the measurement is repeated after switching the positions of the detector and the source array to simulate the case where both sources and detectors consist of a 2-D array of elements. We find that the proposed phased-array method is able to separate cylinders at different depths, thus showing cross-sectioning capabilities.

Equipment Design↗