Multianalyte bioanalytical devices: scientific potential and business requirements.
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Biosensing Techniques↗
Biomedical subjects
Andreas Brecht
Publications and source records attributed to Andreas Brecht.
Online monitoring of solid-phase peptide syntheses on glass-type surfaces using white light interference.
Label-free optical detection methods are of particular value for the investigation of biomolecular interactions. A label-free method based on reflectometric interference spectroscopy is described which enables both the on-line monitoring of solid phase peptide syntheses and subsequently the determination of antibody binding to these peptides without cleavage from the support. The method uses SiO(2) transducers that were modified with diaminopolyethylene glycol. The stepwise coupling of different amino acids to the transducer surface was investigated and the complete monitoring of the synthesis of a viral epitope was performed. The success of the synthesis was proven via binding of a specific monoclonal antibody to the transducer-bound product. In order to demonstrate specificity the binding was inhibited with the same peptide epitope added in solution. The approach is attractive especially in the field of high throughput screening since both the synthesis and the interaction with the biological receptor can be monitored using the same technique.
Amino Acids↗
Optical multiple-analyte immunosensor for water pollution control.
A prototype of a portable optical immunosensor (called river analyser) has been developed. It can be applied for the monitoring of surface water quality. Antibodies carrying a fluorescent label are used for the specific recognition of pollutants, such as frequently applied pesticides. The transduction principle is based on total internal reflection fluorescence (TIRF). The outstanding advantage of the river analyser is, that at least three analytes can be detected simultaneously in one sample. Test cycles and fluid handling are automated and enable unattended measuring.
Calibration↗