Ana Claudia Ramalho | Search PubMed

PubMed2006

The effect of resistance versus aerobic training on metabolic control in patients with type-1 diabetes mellitus.

This study evaluated the effect of aerobic versus resistance training on metabolic control in type-1 diabetes patients. Thirteen non-active patients, ranging in age from 13-30, were submitted to a 12-week aerobic exercise (Group A, n = 7) or resistance training (Group B, n = 6) period. Group A training consisted of a 40 min walk or run and Group B training consisted of resistance exercises three times a week. Blood samples were obtained before and after the 12-week training period. When these samples were compared, results showed that in Group A there were no changes in glycated hemoglobin, lipid profile, fast glucose level or body mass index (BMI). There was, however, a reduction in waist circumference and in average self-monitored blood glucose levels, measured after each exercise session. In Group B, there were no changes in the parameters evaluated. In both groups the total insulin dosage was reduced. As other authors have shown, resistance/aerobic training did not improve glycated hemoglobin in type-1 diabetes patients.

Adolescent↗

PubMedDate not supplied

Estradiol and raloxifene decrease the formation of multinucleate cells in human bone marrow cultures.

Estrogen (E2) deficiency is responsible for increased bone turnover in the postmenopausal period, and it can be prevented by estrogen replacement therapy. The way estrogen acts on bone cells is not fully understood. Human bone marrow cell cultures may be a reliable model for studying the action of steroids on osteoclastogenesis in vitro. We examine the effects of estradiol and Raloxifene, a selective estrogen receptor modulator, on human primary bone marrow cells cultured for 15 days. 17beta-estradiol and Raloxifene significantly decreased the number of tartrate-resistant acid phosphatase multinucleate cells from osteoclast precursors on day 15. Estrogen receptor alpha (ER-alpha) mRNA was present in bone marrow mononuclear cells cultured for 5 days, but there was no estrogen receptor beta (ER-beta) mRNA, suggesting that this effect was mediated by ER-alpha. 15-day cultures no longer contained ER-alpha mRNA, suggesting that estrogen acts on early events of osteoclast differentiation. Finally, 10-8 M 17beta-estradiol has no effect on the release of IL-6 and IL-6-sr into the medium of marrow mononuclear cells cultured for 5 or 15 days. Osteoclast apoptosis was not affected by estradiol or Raloxifene after 15 days of culture under our conditions. In conclusion, we have shown that both estradiol and Raloxifene inhibit osteoclast differentiation in human bone marrow mononuclear cultures. The biological effect that can mimic in vivo differentiation could be mediated through ER-alpha.

Apoptosis↗