Biomedical subjects

Albert P Pisano

Publications and source records attributed to Albert P Pisano.

4 recordsLinked to original sources

PubMed2006

Towards the silicon nanowire-based sensor for intracellular biochemical detection.

A microneedle sensor platform with integrated silicon nanowire tip was developed for intracellular biochemical detection. Because of the virtue of miniaturized size and high sensitivity, this sensor has a great potential for studying individual cell or localized bioenvironment by revealing the pH level and/or enzyme activities. The fabrication of the microneedle sensor was primarily based on conventional silicon processing, where a silicon-on-insulator (SOI) wafer with 50 nm thick (100) p-type Si device layer was used as the substrate. The silicon nanowires of 50 nm height and 50-100 nm width were created by electron beam (E-beam) lithography on the tip of microneedle with good electrical connection to the contact pads for convenient electrical measurement. A three layer structure with base, support cantilever, and needle tip was designed to ensure convenient handling of sensors and minimize the invasive penetration into biological cells. In this paper, we demonstrate a preliminary assessment of this novel intracellular sensor with electrical conductance measurement under different pH levels. It is expected that this sensor with proper chemical modification will enable localized biochemical sensing within biological cells, such as neurotransmitter activities during the synaptic communication between neuron cells.

Biosensing Techniques↗

PubMed2006

Injection molded microfluidic chips featuring integrated interconnects.

An injection molding process for the fabrication of disposable plastic microfluidic chips with a cycle time of 2 min has been designed, developed, and implemented. Of the sixteen commercially available grades of cyclo-olefin copolymer (COC) that were screened for autofluorescence and transparency to ultraviolet (UV) light, Topas 8007 x 10 was identified as the most suitable for production. A robust solid metal mold insert defining the microfluidic channels was rapidly microfabricated using a process that significantly reduces the time required for electroplating. No wear of the insert was observed even after over 1000 cycles. The chips were bonded by thermal fusion using different bonding conditions. Each condition was tested and its suitability evaluated by burst pressure measurements. The COC microfluidic chips feature novel, integrated, reversible, standardized, ready-to-use interconnects that enable operation at pressures up to 15.6 MPa, the highest value reported to date. The suitability of these UV transparent, high pressure-resistant, disposable devices was demonstrated by in situ preparation of a high surface area porous polymer monolith within the channels.

Microfluidic Analytical Techniques↗

PubMed2005

Design of a monolithic piezoelectrically actuated microelectromechanical tunable vertical-cavity surface-emitting laser.

We report the design of a monolithic piezoelectrically actuated microelectromechanical tunable vertical-cavity surface-emitting laser (VCSEL). The main advantages of piezoelectric actuation compared with conventional capacitive techniques are improved wavelength control, reduced external and tilt losses, and lower power supply voltages. The details of the piezoelectric actuation scheme for a 980-nm VCSEL with a variable air gap are described. A tuning range of approximately 35 nm can theoretically be achieved with a 3-V power supply (2 x reduction from that of electrostatic actuation) by use of a 250-microm-long cantilever beam. The proposed actuation mechanism is insensitive to the pull-in phenomenon, therefore improving wavelength control and reducing threshold current. Drastic improvements in power efficiency make it ideal for low-power applications such as all-optical communication, chip-scale atomic clocks, and biological studies.

Journal Article↗

PubMed2004

Continuous on-chip micropumping for microneedle enhanced drug delivery.

Microneedles are promising microfabricated devices for minimally invasive drug delivery applications. Needles can be integrated into a variety of devices. However, any portable drug delivery device with integrated microneedles will need an equally compact means to deliver therapeutics. This work presents microneedles integrated with an on-chip MEMS positive displacement micropump for continuous drug delivery applications. The generation and collapse of thermally generated bubbles with flow rectified by directional check valves are used to achieve net pumping through the device. Visualization methods have observed net flow rates of water out of a microneedle at approximately 2.0 nl/s with a pressure of 3.9 kPa. In addition, continuous pumping was achieved for more than 6 hours with the heaters actuating for over 18 hours (15,000 cycles) without failing.

Drug Delivery Systems↗