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PubMed1990

[Curative effects of modified xiao-jin pills and chemotherapy after surgery in patients with mid and late phase gastric cancer].

From 1986, the authors mainly used Modified Xiao-Jin Pills (MXJP) added with chemical treatment to cure patients in mid and late phase of gastric cancer after operation. 72 patients were verified as gastric cancer by pathological section and phases were divided according to TNM classification which was revised by the national cooperative group of gastric cancer in 1978. The patients were randomly divided into curative and control groups. The two groups were basically similar in sex, age, clinic pathological phase and operation. One month after operation, the two groups received the same treatment except that the control group did not take MXJP. The indexes of observation consisted of living rate, substance of tongue and 5 indexes of blood coagulation (AT-III, Fn, FA, VIII R:Ag and beta TG). The curative effects showed that MXJP could enhance gastric cancer patients living rate for 1, 1.5 and 2 years among which it was obvious for phase IV's gastric cancer patients to enhance 1 year's living rate (P less than 0.05), and lower the negative rate of substance of tongue (P less than 0.001). The indexes of AT-III, Fn FA and VIII R:Ag and beta TG of gastric cancer patients had changed for the better (P less than 0.05). The results showed that MXJP can enhance the capacity of body to antagonize coagulation fibrinolysis, remove blood stasis and further enhance the living rate of patients. MXJP has better curative effects of short term.

Adult↗

PubMed1989

Reconstitution of murine resident peritoneal macrophages for antibody-dependent cellular cytotoxicity by homologous serum Clq.

Mouse resident peritoneal macrophages (PM) were reconstituted in their response to activation for antibody-dependent cellular cytotoxicity (ADCC) for sheep erythrocyte targets (SRBC) by subhemolytic dilutions of homologous or autologous sera. ADCC-responsive inflammatory PM were largely unaffected in their activation by exogenous serum. Augmentation of resident PM for ADCC by homologous serum was correlated with the complement-activating potential of the mouse monoclonal anti-SRBC IgG isotype in that serum augmented IgG gamma 2a greater than IgG gamma 2b much greater than IgG gamma 1. The active component of mouse serum was heat-labile at 56 degrees C for 30 min and was present in both C5-deficient AKR and C5-sufficient homologous C3H mouse sera. Western blot analysis of the cell lysates for Clq confirmed that oil-elicited and thioglycollate-elicited inflammatory PM had greater levels of endogenous Clq than did resident PM which correlated with their innate responsiveness for ADCC activation. Depletion of Clq from serum by immunoprecipitation with IgG antibody to Clq or by ion exchange chromatography removed the active reconstituting activity for ADCC. Purified mouse Clq (0.4 microgram) partially replenished the ADCC augmenting activity of Clq-depleted AKR mouse serum. SRBC targets preopsonized with IgG gamma 2a and purified mouse Clq (0.075-5.0 microgram/ml) fully reconstituted the ADCC response of resident PM similar to homologous serum indicating that the major active component of serum was Clq. Thus resident PM with low endogenous levels of Clq were reconstituted for ADCC by the addition of exogenous Clq, whereas inflammatory PM with sufficiently high endogenous levels of Clq were not further enhanced by exogenous Clq. Our findings indicate that Clq may provide an essential second signal in concert with Fc receptor binding of IgG to initiate ADCC activation of macrophages.

Animals↗