Biomedical subjects

A Z Yumbul

Publications and source records attributed to A Z Yumbul.

4 recordsLinked to original sources

PubMed1995

A sensitive staining method for NORs.

A sensitive staining method for nucleolar organizer regions (NORs) is described, using blue toning of AgNORs. NORs are loops of DNA which are transcribed into ribosomal RNA. NORs can be demonstrated by staining with silver nitrate, since NOR-associated proteins are argyrophilic, producing structures termed AgNORs. Normal blood lymphocytes were stained with both methods. The number and resolution of NORs increased 2-3 times by blue toning (30 mmol/l FeCl3, 11 mmol/l potassium hexacyanoferrate(III), and 33 mmol/l oxalic acid) compared with silver staining. A significant difference in the number of NORs was noticed between silver-stained and blue-toned cells (P < 0.001). The blue toning technique thus appears to be more sensitive in detecting NORs than the AgNOR method and may prove a useful alternative for applications in histopathology.

Chlorides↗

PubMed1994

Hydronephrotic type of bilateral multicystic dysplastic kidney. An unusual case.

25 year-old pregnant woman was admitted at week 29 of fetal development for routine ultrasonic evaluation of growth. Sonography of the fetal body revealed two cystic anechoic septated structures bilaterally. At autopsy of the stillborn neonate pathology confirmed congenital multicystic dysplastic kidney. We believe this is the first case reported.

Abnormalities, Multiple↗

PubMed1994

Primary ovarian pregnancy. A case report.

A primary ovarian pregnancy was diagnosed in a patient with intrauterine device. Primary ovarian pregnancy which is a rare form of ectopic pregnancy, was reviewed according to etiology, pathogenesis and improvements in diagnosis and therapy. In this article, our case was multiparous and used intrauterine device for 5 years. Ovarian pregnancy was diagnosed before operation. This diagnosis was verified histologically and she was treated by oopherectomy.

Adult↗

PubMedDate not supplied

Nucleolar organizer regions in lymphomas: a quantitative study.

Using a silver staining technique, nucleolar organizer region-associated proteins (Ag-NORs) have been studied in paraffin sections of 76 non-Hodgkin's lymphomas, five normal lymph nodes, and five < > lymph nodes. The mean number of nucleolar organizer regions per nucleus was 1.19 (SD:0.09) for normal lymphocytes, 3.04 (SD:0.14) for reactive lymph nodes, 2.79 (SD:0.44) for low-grade lymphomas, 6.33 (SD:1.58) for intermediate-grade lymphomas, and 10.53 (SD:1.97) for high-grade lymphomas. There were highly significant differences in Ag-NOR counts among the groups (p < 0.001). The Ag-NOR regions were often observed in nuclei in areas where nucleoli themselves were invisible. It is suggested that this method is useful in diagnostic histopathology and in differentiation of the grade of lymphomas.

Humans↗