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Biomedical subjects

A Wright

Publications and source records attributed to A Wright.

At least 379 records · Page 21Linked to original sources
PubMed1971

Mechanism of conversion of the salmonella O antigen by bacteriophageepsilon 34.

The structural determinants for antigen 34 in the E group salmonella are glucosyl substituents on the galactosyl units of the O antigen which has a mannosylrhamnosylgalactose repeating sequence. The temperate bacteriophage epsilon(34) brings about the production of antigen 34. It has been shown here that glucose is transferred from uridine diphosphate glucose to the O antigen via a glucosyl-lipid intermediate in a two-step reaction. Glucose is linked through carbon 1 to the lipid by a phosphodiester bridge, the glucosyl bond having the beta-anomeric configuration. The lipid is a C(55)-polyisoprenoid alcohol, each isoprene unit having one double bond. It is the same lipid which is involved in the synthesis of the O antigen repeating sequence.

Antigens↗
PubMed1971

Isolation and haracterization nonconverting mutants of bacteriophage epsilon 34.

epsilon(34) Is a converting bacteriophage which brings about a glucosylation of the O antigen in the E group salmonella, thus producing antigen 34. Uridine diphosphate glucose is the precursor of antigen 34 and a glucosyl-lipid is an essential intermediate in this process. Mutants of epsilon(34) which cannot produce antigen 34 have been isolated. Cells lysogenic for these mutants are of two types. Type 1 mutant lysogens cannot form the glucosyl-lipid intermediate; those of type 2 can form glucosyl-lipid but are unable to transfer glucose from the lipid to the O antigen.

Adsorption↗