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Biomedical subjects

A Wille

Publications and source records attributed to A Wille.

At least 37 records · Page 2Linked to original sources
PubMed1996

Community analysis of the bacterial assemblages in the winter cover and pelagic layers of a high mountain lake by in situ hybridization.

The bacterial community structure in the winter cover and pelagic zone of a high mountain lake was analyzed by in situ hybridization with fluorescently labeled rRNA-targeted oligonucleotide probes. Cells fixed on membrane filters were hybridized with a probe specific for the domain Bacteria as well as with probes for the alpha, beta, and gamma subclasses of the class Proteobacteria and the Cytophaga-Flavobacterium group. The fraction of bacteria detectable after hybridization with the bacterial probe EUB ranged from 40 to 81% of 4(prm1),6-diamidino-2-phenylindole (DAPI) counts. The bacterial assemblage varied considerably between and within different habitats (snow, slush, and lake water) but was in most cases dominated by members of the beta subclass (6.5 to 116% of bacteria detectable with probe EUB). The sum of bacteria hybridizing with group-specific probes was usually lower than the fraction detectable with probe EUB. Image analysis was used to characterize morphology and the size-specific biomass distribution of bacterial assemblages, which clearly separated the three habitats. Although the measured secondary production parameters and the fraction of 2-(p-iodophenyl)-3-(p-nitrophenyl)-5-phenyltetrazolium chloride-reducing bacteria varied by more than an order of magnitude in the different slush and pelagic layers, detectability with the fluorescent probe EUB was constantly high. Physiological strategies of bacteria under nutrient limitation and at low temperatures are discussed in the context of the ribosome content of single cells. This study confirms the suitability of fluorescently labeled rRNA-targeted probes for the characterization of bacterial population structures even in oligotrophic habitats.

Journal Article↗
PubMed1993

Excretion of urinary enzymes after extracorporeal shock wave lithotripsy: a critical reevaluation.

The excretion of the urinary enzymes alanine aminopeptidase, alkaline phosphatase, gamma-glutamyl-transferase and N-acetyl-beta-D-glucosaminidase, and the 99mtechnetium-diethylenetriaminepentaacetic acid isotope clearance were studied in 35 patients treated with extracorporeal shock wave lithotripsy (ESWL*). Enzyme measurements were made before and consecutively on days 1, 2 and 5, and at 3 months after treatment. A control group investigated at the same intervals was included. Posttreatment enzyme values were not significantly different from those before treatment except for alkaline phosphatase on day 1 after ESWL. Some individuals had short-term increases of enzymuria that were greater than biological variations in the control group. The transient changes of enzymuria after ESWL had no predictive value for kidney function, since no decreased renal function was observed in individual patients with high enzyme excretions after ESWL.

Acetylglucosaminidase↗
PubMed1989

Mechanism of recovery from acute virus infection. VIII. Treatment of lymphocytic choriomeningitis virus-infected mice with anti-interferon-gamma monoclonal antibody blocks generation of virus-specific cytotoxic T lymphocytes and virus elimination.

In acutely infected mice the lymphocytic choriomeningitis (LCM) virus multiplies to high titers in essentially all tissues. Around day 6, virus clearance sets in, which has previously been shown to be mediated by CD8+ cytotoxic T lymphocytes (CTL), probably by releasing (or inducing other cells to release) anti-viral cytokines. To ascertain whether interferon-gamma plays a role, infected mice were injected once i.v. with monoclonal antibody known to neutralize this lymphokine, and the effect this had on both termination of the infection and development of LCM virus-specific CTL was determined. Administration 1 day after infection blocked virus elimination from spleen and liver and decreased the generation of CTL; also, limiting dilution analysis revealed absence of activation of CTL precursors. In contrast, when the antibody was given 3 days after or 1 day before the virus, neither clearance nor generation of CTL was measurably affected. Furthermore, the antiviral effect of immune spleen cells after their transfer into infected recipients was not altered by treatment of the latter with monoclonal antibody. We conclude that in the generation of LCM virus-specific CTL an early event is dependent on constitutively produced interferon-gamma; when its activity is blocked, CTL do not mature, resulting in the mouse's inability to terminate the infection.

Animals↗
PubMed1988

[Clinical practice of a child and adolescent psychiatric service as evaluated by parents].

A follow-up study was conducted at an outpatient child psychiatry facility on parents' satisfaction with the quality and effectiveness of the treatment provided their children. The selection of the sample (N = 105), the methods used and the high response rate of 80% are discussed. The parents who did not return the questionnaire were interviewed later by telephone. For the sample as a whole, about 75% of the parents made a positive assessment of the service provided by the clinic and the improvement in their child's situation and general development. The initially non-responding parents were less positive in their assessment than the responders. The conclusions to be drawn from these findings are discussed.

Adolescent↗
PubMed1986

[Psychological military fitness--a catamnestic study].

1980 a psychiatric examination was made of a pre-selected collective (recruits of the tank-corps, ordinance-corps and highway police). Now, three years later, we have re-examined the 96 recruits regarding their psychic qualifications and military suitability and made a report. The methods and the results are illustrated and discussed in detail therein.

Adaptation, Psychological↗
PubMed1985

[Loyalty conflicts in children of divorce].

Divorce is dealt with as a social phenomenon, including the effects of divorce on the children concerned and on their development. In case studies the practical work with divorce families is described in detail. Depending on the possibilities and the readiness of the involved parents, different therapeutical approaches can be taken in order to diminish the loyalty conflicts of divorce children: family-therapeutical work on the symptomatic behaviour of the child, divorce therapy as such with the parents, parent counselling limited to the parental level.

Adolescent↗
PubMed1984

[Individual psychotherapy--family therapy in children and adolescents].

The theoretical basis, the therapeutical approach and the methods of individual psychotherapy and of family therapy are presented. Possibilities and limitations, advantages and disadvantages of the two forms of therapy are discussed and illustrated by means of case histories. It is pointed out that the question of indication can only be solved if on the one hand the patient "individual" and the patient "family" are considered, and if the therapist with his theoretical and practical possibilities is included on the other hand.

Adolescent↗
PubMed1983

[Comparative psychiatric study of recruits].

From a group of 1780 recruits, 30 recruits who had been showing behavioural disorders but had remained in army service, were compared with a control group of recruits who had never demonstrated any deviant behaviour. This was done in order to gain information of the performance of the examined persons in respect of their physical capacities, emotional patterns, learning abilities and efficiency, consumption patterns, personal contacts and sociological integration. The article describes the methodic approach and discusses the results. In conclusion, the possibility of prophylactic sociomedical activity in the school of recruits is brought up for discussion, was well as the question of their fitness for active military service.

Adaptation, Psychological↗
PubMed1975

[Effects of the ashes of the Irazu volcano (Costa Rica) on various insects].

A study of the 1963-1965 eruptions of the Irazú volcano (Costa Rica) showed that the ashes altered the ecological conditions of a great number of insects. Experiments suggest a mechanical action of the abrasive particles on the epicuticle, making it permeable, and thus accelerating dehydration. Other insects, such as the honey bee (Apis mellifera), were not affected externally but through the ingestion of ash-contaminated nectar and by the massive destruction of the vegetation on which they depended. Great destruction of bee populations was observed during 1964 and 1965-66 in the San José area. On the other hand, the volcanic ash apparently favored other insects, especially those protected by a waxy secretion, and by the possible elimination of their natural enemies, to such proportions that they became pests of economic significance.

Costa Rica↗
PubMedDate not supplied

[Voice dialogue--dialogue of voices].

Voice Dialogue is an integration of different therapeutic methods (Gestalttherapy, Psychodrama, Psychology of C.G. Jung, Transactional Analysis, Psychosynthesis). It is a therapeutic method, which allows in a rather humoristic way, without provoking lots of resistance, to contact the subpersonalities of a client. This will objectify and bring into awareness the different subpersonalities, as well as the disowned parts of the personality. Therefore we will have the chance to deal with these parts in a creative way without the Ego interfering critically. So a new balance can be found between the different subpersonalities and the Ego. Voice Dialogue can be applied very well in single, partner or family therapy and has worked successfully with grown-ups and especially with youngsters.

Adolescent↗