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Biomedical subjects

A Wang

Publications and source records attributed to A Wang.

At least 253 records · Page 14Linked to original sources
PubMed1994

Plasma soluble interleukin-2 receptor in patients with primary myelofibrosis.

Using en enzyme-linked immunosorbent assay (ELISA) test, the level of soluble Tac peptide, one chain of the human interleukin-2 receptor, was measured in the plasma of 26 patients with primary myelofibrosis (MF), seven patients with polycythaemia vera and 11 normal controls. The plasma soluble interleukin-2 receptor (sIL-2R) was found to be significantly elevated in patients with primary MF compared to polycythaemia vera or controls (P < 0.001), while the plasma sIL-2R of patients with polycythaemia vera also was found to be significantly elevated compared to controls (P < 0.01). The significantly elevated value of sIL-2R seen in primary MF may be secondary to T cell activation resulting from autoimmune phenomena, and myeloblast activation with release of sIL-2R may also be a contributing factor. In primary MF, plasma sIL-2R levels were also found to be correlated to survival, circulating blast cell counts, and thrombocytopenia, but not to white blood cell counts, LDH levels, degree of marrow fibrosis, or degree of splenomegaly. Patients with primary MF with higher titre of plasma sIL-2R had a shorter survival. Further studies involving more patients and longer follow-up may substantiate that plasma sIL-2R is an important prognostic indicator in primary MF.

Biomarkers↗
PubMed1994

Use of antineutrophil cytoplasmic autoantibodies in diagnosing vasculitis in a Chinese patient population.

Antineutrophil cytoplasmic autoantibodies (ANCA) have been used as markers of systemic vasculitides, including microscopic polyarteritis (MPA) and Wegener's granulomatosis. The diagnostic potential of ANCA assays together with antibodies against the neutrophil enzymes myeloperoxidase (MPO) and proteinase 3 for detecting a systemic vasculitis was tested in a Chinese patient population. 672 sera were received for ANCA assay, and ANCA detected by indirect immunofluorescence was positive in 73 sera from 42 patients. Of the 42 patients, 3 had cytoplasmic ANCA, while 39 had a perinuclear pattern. There was no patient with Wegener's granulomatosis. Two cytoplasmic ANCA positive patients suffered from ulcerative colitis. Another cytoplasmic ANCA positive patient was a carrier of human immunodeficiency virus. Of the 39 perinuclear ANCA positive patients, 10 had MPA. Eight of them were tested for anti-MPO antibody, and all were positive. Other immune disorders that were perinuclear ANCA positive included: 13 patients with systemic lupus erythematosus, 3 with mixed connective tissue disease, 1 with Goodpasture's syndrome, 2 with inflammatory bowel disease, and 2 patients with IgA nephropathy. Anti-MPO antibody was not specific for MPA, and 7 out of the 13 patients with systemic lupus erythematosus were anti-MPO antibody positive. Our study suggests that ANCA and anti-MPO antibody are not specific for MPA in a Chinese population. They would alert the clinician of the possibility of vasculitis, but a clinicopathological correlation is essential in making the diagnosis.

Adolescent↗
PubMed1994

Experimental study on effect of traditional bone-knitting drugs on traumatic gu bi (bone-rheumatoid lesions).

Traditional bone-knitting drugs (TBKD) are used frequently in the treatment of bone fractures. An animal model of Gu Bi (bone-related rheumatoid lesions) with defects of articular cartilage as the main lesion was developed by surgical trauma under the stereomicroscope in Wistar rats and treated with TBKD. The results showed that TBKD could promote proliferation of cartilage cells around the traumatic site and improve the osteogenetic function in the traumatic state, suggesting that TBKD can be used in combination with other Chinese drugs to treat Gu Bi, including bony lesions of the late-stage rheumatoid arthritis and bony arthritis. But the experimental results also suggested that TBKD might have an effect of promoting development of inflammation, and therefore, should be used with care in the acute phase of inflammation.

Animals↗
PubMed1994

[The cloning of threonine operon in vivo and mutagenesis in vitro in E. coli].

Report here was the selection of the E. coli strain producing 1.2% threonine. The wild type threonine operon was cloned in vivo and subcloned in vitro. The subclone was mutagenizied in vitro and a clone pTHR12-9-1 producing threonine was obtained. Also the effect of pTHR12-9-1 on threonine yield of hosts producing different amount of threonine was studied. We found that pTHR12-9-1 could promote the production of threonine in lower yield hosts, but it reduced the production of threonine in higher yield hosts. The phenomenon was discussed.

Cloning, Molecular↗
PubMed1994

[Investigation on HBV-M among hospital workers and observation on the efficacy of vaccination against type B hepatitis].

An epidemiological study on HBV infection among 315 staff of PUMC Hospital was carried out with detection of HBsAg, anti-HBs and anti-HBc by ELISA. The results showed that 138 persons had one or more markers of HBV infection and an overall HBV prevalence rate 43.8%. The positive rates of HBsAg anti-HBs, anti-HBc were 6.3%, 28.9% and 8.6%, respectively. This indicates that HBV infection is a risk factor for medical staff, but there was no significant difference among medical, surgical and other members. In 1992, 177 workers whose HBV markers were negative received 3 doses of HB vaccine at 0, 1 and 6 months, respectively. 91.3 percent of them showed an anti-HBs response one month after the last inoculation. The result shows that our national made HB vaccine is safe and immunogenic.

Adult↗
PubMed1993

Inhibitory effects of magnesium hydroxide on c-myc expression and cell proliferation induced by methylazoxymethanol acetate in rat colon.

The effects of magnesium hydroxide were examined on methylazoxymethanol (MAM) acetate-induced c-myc expression and cell proliferation of colonic mucosal epithelium in rats. Rats were divided into four groups and treated as follows: MAM acetate alone (25 mg/kg i.p. injection, five times, once a week for 5 weeks), MAM acetate and feeding of 0.2% magnesium hydroxide in diet, magnesium hydroxide alone and non-treatment. At 4, 8 and 16 weeks after the start of experiment, 10 rats in each group were sacrificed. Magnesium hydroxide inhibited the MAM-induced expression of c-myc proto-oncogene, and also suppressed the increased bromodeoxyuridine (BrdU) and proliferating cell nuclear antigen (PCNA) labelling indexes induced by MAM acetate in colon mucosa in initiation and post-initiation phase. These results suggest that the anti-carcinogenic effect of magnesium hydroxide on rat colon carcinogenesis induced by MAM acetate may be related to the inhibition of the carcinogen-induced expression of c-myc proto-oncogene and cell proliferation.

Animals↗
PubMed1993

Cloning and characterization of a human Mac-2-binding protein, a new member of the superfamily defined by the macrophage scavenger receptor cysteine-rich domain.

We have purified and sequenced a secreted glycoprotein from both the human breast carcinoma cell line, SK-BR-3, and human breast milk. The native protein binds specifically to a human macrophage-associated lectin known as Mac-2. This Mac-2 binding protein (Mac-2-BP) has an apparent native molecular mass of several million daltons and contains subunits of 85-97 kDa that are very susceptible to proteolysis at a dibasic cleavage site. Western analysis suggests that Mac-2-BP is found in serum, semen, saliva, urine, and tears, in addition to breast milk. The gene encoding Mac-2-BP was cloned from a cDNA bank of a human monocytic cell line, using degenerate PCR primers based on the protein sequence. Recombinant Mac-2-BP was expressed in Cos cells and secreted as a high molecular weight complex. The cDNA clone encodes a mature protein of 567 amino acids, preceded by an 18-amino acid leader. The mature protein contains 16 cysteines and has seven potential N-linked glycosylation sites. The first 106 amino acids represent a domain that is highly similar to an ancient protein superfamily defined by the macrophage scavenger receptor cysteine-rich domain.

Amino Acid Sequence↗
PubMed1993

Successful treatment of amoebic meningoencephalitis in a Chinese living in Hong Kong.

Primary amoebic meningoencephalitis due to Naegleria fowleri was found in a 38-year-old Chinese man living in Hong Kong who presumably acquired the infection from swimming in a hot spring in neighbouring China. Amoebic cysts were identified in tissue taken from a brain abscess. The patient responded to surgical drainage and a 6-week course of amphotericin B, rifampicin and chloramphenicol. This is one of 6 cases of successful treatment of primary amoebic meningoencephalitis documented in the medical literature.

Adult↗
PubMed1993

Nursing diagnoses for psychiatric patients in China.

This report describes the development of a preliminary Chinese psychiatric classification system with nine categories and 211 separate diagnoses, and the corresponding diagnostic interview that enables psychiatric nurses in China to systematically assess the presence or absence of the various diagnoses. Using this interview with 30 representative inpatients, the authors identified 152 of the 211 (72%) diagnoses. On average, each patient was given 26 different nursing diagnoses. The interrater reliability of the four nurses who independently coded the diagnostic interview for these 30 patients was excellent: in over 77% of the identified diagnoses the generalized kappa was greater than 0.75. These findings show that further work on this classification of nursing diagnoses is warranted.

Adolescent↗
PubMed1993

Inhibition of 4-nitroquinoline-1-oxide-induced rat tongue carcinogenesis by the naturally occurring plant phenolics caffeic, ellagic, chlorogenic and ferulic acids.

The modifying effects of dietary administration of the plant phenolic antioxidants caffeic acid (CA), ellagic acid (EA), chlorogenic acid (CGA) and ferulic acid (FA) during the initiation phase on 4-nitroquinoline-1-oxide (4-NQO)-induced tongue carcinogenesis and on the number and area of silver-stained nucleolar organizer region proteins (AgNORs), a new cell proliferation marker, of the tongue squamous epithelium were investigated in male F344 rats. Rats were fed the diet containing 500 p.p.m. CA, 400 p.p.m. EA, 250 p.p.m. CGA or 500 p.p.m. FA for 7 weeks. One week after the commencement of the diets, 4-NQO (20 p.p.m.) was administered in the drinking water for 5 weeks. Feeding of four phenolic compounds significantly reduced the incidences of tongue neoplasms (squamous cell papilloma and carcinoma) and preneoplastic lesions (hyperplasia and dysplasia) by 32 weeks, and rats fed CA or EA had no tongue neoplasms. The number and area of AgNORs per nucleus were decreased significantly by dietary treatment with these four phenolics. Thus, CA, EA, CGA and FA inhibited the tongue carcinogenesis induced by 4-NQO when they were administered concurrently with the carcinogen. These results might suggest possible application of these natural substances for cancer chemoprevention in tongue in addition to other tissues (skin, lung, liver and esophagus).

4-Nitroquinoline-1-oxide↗
PubMed1993

Plasma exchange in the treatment of early recurrent focal glomerulosclerosis after renal transplantation. Report and review.

We report a 29-year-old female with recurrent focal glomerulosclerosis (FGS) presenting as heavy proteinuria immediately following renal transplantation. From day 13 after transplantation, daily plasma exchange was performed 6 times. Proteinuria decreased from 13 to 0.2 g/day after plasma exchange and remained to be less than 0.1 g/day even 20 months thereafter. Our review of the literature on the use of plasma exchange in treating recurrent FGS suggests this procedure is not useful in patients whose allograft biopsy showed advanced glomerular sclerotic lesions. We propose early plasma exchange may be a therapeutic option in those patients who have no sclerotic lesions in their allograft biopsy.

Adult↗
PubMed1993

Effects of galactose feeding on aldose reductase gene expression.

Aldose reductase (AR) is implicated in the pathogenesis of the diabetic complications and osmotic cataract. AR has been identified as an osmoregulatory protein, at least in the renal medulla. An outstanding question relates to the response of AR gene expression to diet-induced galactosemia in extrarenal tissues. This paper shows that AR gene expression in different tissues is regulated by a complex multifactorial mechanism. Galactose feeding in the rat is associated with a complex and, on occasions, multiphasic pattern of changes in AR mRNA levels in kidney, testis, skeletal muscle, and brain. These changes are not in synchrony with the temporal sequence of changes in tissue galactitol, galactose, and myoinositol concentrations. Moreover, galactose feeding results in changes in tissue AR activities that are not related, temporally or quantitatively, to the alterations in tissue AR mRNA or galactitol levels. It is concluded that AR gene expression and tissue AR activities are regulated by mechanisms that are not purely dependent on nonspecific alterations in intracellular metabolite concentrations. This conclusion is supported by the finding that chronic xylose feeding, despite being associated with intracellular xylitol accumulation, does not result in alterations in AR mRNA levels, at least in the kidney.

Aldehyde Reductase↗