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Biomedical subjects

A Vijayalakshmi

Publications and source records attributed to A Vijayalakshmi.

6 recordsLinked to original sources
PubMed2002

Effect of long-term dietary manipulation on the aggregation of rat lens crystallins: role of alpha-crystallin chaperone function.

PURPOSE: To investigate the effect of food, protein, and vitamin restriction on the susceptibility of lens crystallins to aggregation and chaperone activity of alpha-crystallin. METHODS: Thirty day old Wistar/NIN rats were maintained on regular rodent diet (C), 50% food restriction (FR), 75% protein restriction (PR), and 50% vitamin restriction (VR) diet for 20 weeks. At the end, alpha-, beta-, and gamma-crystallins were isolated from the lenses of these animals and subjected to in vitro aggregation induced by oxidation, UV irradiation and heat. Aggregation and chaperone activity was assessed by light scattering methods. RESULTS: Dietary restriction has been shown to extend the mean and maximum life span and retard age-related diseases, including cataract. In this study, we demonstrate that while beta- and gamma-crystallins isolated from FR and PR groups were less susceptible to in vitro induced aggregation, beta- and gamma-crystallins from the VR group were more susceptible, compared to controls. Alpha-crystallin from any of the groups did not shown a considerable amount of aggregation. On the other hand, the chaperone activity of alpha-crystallin from FR and PR groups was not significantly different from controls. However, alpha-crystallin from the VR group demonstrated substantially higher chaperone activity than controls. CONCLUSIONS: These results indicate that while food and protein restriction appear to lower the susceptibility of beta- and gamma-crystallins towards aggregation, vitamin restriction tends to increase the aggregation. Chaperone activity of alpha-crystallin is affected (improved) by only vitamin restriction.

Animals↗
PubMed2001

Monitoring of allethrin, deltamethrin, esbiothrin, prallethrin and transfluthrin in air during the use of household mosquito repellents.

Three types of mosquito repellent [two different mosquito coils containing allethrin 0.1% w/w and transfluthrin 0.03% w/w, an aerosol sample containing a combination of two pyrethroid molecules (deltamethrin 0.02% w/w + allethrin 0.13% w/w) and two different mosquito mats containing esbiothrin 2.0% w/w and prallethrin 1.5% w/w as active ingredients] were individually subjected to use in a closed room. Air samples from the room were drawn at different time intervals (15, 30 and 45 min, and 1, 2, 4, 6 and 8 h) uniformly from three different positions in the room (top, middle and bottom) with the pyrethroid contents analysed using gas chromatography-electron capture detection (GC-ECD). Analysis of air samples showed maximum concentrations of the pyrethroid residues allethrin (0.0120 ppm), transfluthrin (0.0134 ppm), deltamethrin (0.0057 ppm), allethrin (0.080 ppm), esbiothrin (0.015 ppm) and prallethrin (0.0138 ppm) within 30-45 min of use. The drop in residue content was significant with time. At the end of a 6 h period, most of the residues had dissipated to below 0.0001 ppm. Further significant differences were observed in the residue contents tested at different points within the room. Studies were compared with the experimental results obtained when the mosquito repellents were tested with air circulation in the room.

Air Movements↗
PubMed1999

Probing Polyethylene Glycol-Phospholipid Membrane Interactions Using Enzymes.

Interaction of polyethylene glycols (PEGs) with phospholipid membranes leads to aggregation and fusion of membranes. The structural basis of these events in membranes, especially in contact with low-molecular-weight PEGs, is uncertain. Using phsopholipases, a class of interfacially active enzymes, we demonstrate enhanced accessibility of lipid hydrophobic portions in the presence of PEGs. All three phospholipases, i.e., A(2), C, and D, show enhancement of activity in the presence of PEGs. Enhancement of activity does not depend on the size of the vesicle or the presence of proteins in the membrane. Fluorescence quenching of probes buried in the membrane supports the phospholipase data. The utility of phospholipases as probes to monitor local and fine structural changes in the membranes is discussed. Copyright 1999 Academic Press.

Journal Article↗