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Biomedical subjects

A Vierucci

Publications and source records attributed to A Vierucci.

At least 91 records · Page 5Linked to original sources
PubMed1992

VIP restores natural killer cell activity depressed by hepatitis B surface antigen.

Vasoactive intestinal peptide (VIP) has recently been shown to bind to human lymphocytes and modulate immune functions. The ability of VIP in restoring natural killer (NK) cell activity depressed by hepatitis B surface antigen (HBsAg) has been investigated in the present research. Human lymphocytes were incubated with HBsAg and, after washing, a 4-hr cytotoxicity assay was performed. VIP was coincubated with lymphocytes during the preincubation with HBsAg or, alternatively, throughout the cytotoxicity assay. The study revealed that VIP, either preincubated or coincubated in the 4-hr assay, strongly restores NK cell activity depressed by viral antigen. This is noteworthy considering that a number of lymphocyte modulators such as interferons fail in restoring viral-dependent NK cell activity depression. In contrast with previous reports, even when coincubated in the 4-hr assay, VIP is a strong activator of NK cell activity. Further studies will be required to understand which mechanisms are involved in the interrelation between VIP and NK cells during viral infections.

Cytotoxicity, Immunologic↗
PubMed1991

Five-year follow-up of vaccination against hepatitis B virus in newborns vaccinated with a reduced number of doses.

The protective efficacy and long-term immunogenicity of a schedule with a reduced number of vaccinations in infants born to mothers positive for hepatitis B surface antigen (HBsAg) has been investigated: 494 infants immunized with a plasma derived hepatitis B vaccine (Hevac B Pasteur) at day 20 after birth and 2 months later were studied. Anti-HBs seroconversion rate was 96% after the second dose, a value similar to that obtained in older children. At 5 years of life anti-HBs at protective levels was still 95.8%. In conclusion, we suggest that this effective and economical reduced vaccination schedule, which before this study was applied to older children, can be used in newborns from HBsAg positive mothers with good protective efficacy shifting the beginning of active immunization from birth to day 20.

Age Factors↗
PubMed1990

False diagnosis of non-A/non-B hepatitis hiding two cases of cystic fibrosis.

We report two cases of children followed for many years with an original diagnosis of non-A/non-B hepatitis. One child developed serious cirrhosis with portal hypertension. Long-term observation of the course of their hepatic disease provided the diagnosis of cystic fibrosis. These cases demonstrate that cystic fibrosis though rarely presenting with initial hepatic signs, can manifest itself with only long-term hepatic symptoms. We therefore strongly recommend analysis of sweat chloride concentration in cases of hepatic disease of unknown origin.

Adolescent↗
PubMed1990

Allergy to different fish species in cod-allergic children: in vivo and in vitro studies.

The presence of a positive clinical history and skin test (ST) results for 17 fish species (anchovy, bass, carp, dogfish, eel, gilthead, mackerel, mullet, perch, red mullet, salmon, sardine, sole, tench, toothed gilthead, trout, and tuna) were investigated in 20 children with cod-positive clinical history, ST, and RAST, and in 40 children positive to one or more foods different from cod (cow's milk, chicken egg white, peanut, and tomato). In cod-positive children, positive clinical history (60%) and ST (85%) to fish species were more frequent than in cod-negative children (7.5% and 10% respectively). In cod-positive children, a high frequency of positive STs to eel (85%) and to bass, dentex, sole, and tuna (55%) was observed. Positivity to dogfish (10%) was the least frequent. RAST-inhibition experiments suggested the presence of cross-reacting antigen(s) in cod, bass, dentex, eel, sole, and tuna. Results of this study demonstrate that cod allergy might be, on the whole, a reliable index of fish allergy, but cod-positive children may perhaps tolerate some other species, which will have to be tested for possible inclusion in their diet.

Animals↗
PubMed1990

Modulation by human milk of IgG subclass response to hepatitis B vaccine in infants.

The influence of breast and formula feeding on specific anti-hepatitis B surface antigen (HBsAg) IgG subclass production and distribution has been investigated in 40 healthy infants, born to HBsAg-positive mothers and vaccinated against hepatitis B virus (HBV). Twenty children were bottle fed and 20 were breast fed. Specific subclasses were detected at the 4th and 12th months using an enzyme-linked immunosorbent assay with monoclonal antibodies. A defect in total IgG and IgG subclasses was previously excluded. Significant differences were observed both at the 4th and 12th months for IgG1 and IgG2. Breast-fed infants had significantly higher levels of specific IgG2 (about three times higher), while IgG1 levels were significantly higher in formula-fed infants. Anti-HBsAg IgG4 levels were always higher in bottle-fed infants, but a statistical significance was never present. No difference was found in specific IgG3 levels. This study reports the evidence that breast feeding influences specific IgG subclass synthesis against a viral antigen and suggests an immunologic modulation of the response to vaccines dependent not only on age but also on factors present in human milk.

Adult↗
PubMed1989

Food allergy in preterm infants fed human milk.

In 80 preterms aged 9-24 months (mean age: 15.9) and in 80 sex- and age-matched full-terms the frequency of atopic diseases and of positive skin tests to 8 food and 6 inhalant allergens was determined. The two groups did not differ as to overall percentages of cutipositive subjects and patients with atopic diseases. In particular, frequencies of positive skin tests to foods and of atopic dermatitis (the peak prevalence of which occurs early in infancy) were similar in preterm (16.2 and 7.5%, respectively) and full-term (13.7 and 5.0%, respectively) infants. We suggest that preterm infants fed human milk are not at increased risk of developing food allergy and related diseases and that the absorption of antigens through the immature intestine does not seem to favor the development of an IgE sensitization to foods.

Animals↗
PubMed1989

[Importance of a particular formula of soybean milk without S2 globulin, in the treatment of allergy to cow's milk proteins].

Replacing cow's milk with a soybean milk is one of the first measures which are commonly taken with babies suffering from cow's milk protein allergy. Unfortunately soybean can induce sensitization, too, with a variable percentage (ranging from 12 to 80%) according to the different Authors, due to the presence of allergenic proteins, among which the most important one seems to be S2 globulin. An excellent tolerability has been reported in a study carried out with 20 babies suffering from cow's milk protein allergy besides a good palatability of the product with a solution of symptoms in 87.3% of the cases. Only in 16.7% of the treated babies intolerance has been reported and therefore we can conclude that soybean milk has proved to be an excellent substitute of cow's milk in babies suffering from cow's milk protein allergy.

Child, Preschool↗
PubMed1988

Foods and respiratory allergy.

Foods may induce respiratory symptoms by both reaginic and nonreaginic mechanisms. Asthma is one of the most common respiratory manifestations in children, and it is well known that many factors may provoke an attack. When considering the relationship between foods and asthma, we must keep in mind that food allergy may coexist with an inhalant allergy and that other nonallergens, such as pollutants, smoke, or additives, may modulate or modify bronchial reactivity and thus favor the food allergen action. In a study using clinical history, prick test, radioallergosorbent test, and double-blind food challenge, we demonstrated respiratory symptoms related to food allergy in 13 of 140 (9.2%) children with asthma. Asthma, in particular, was demonstrated in 8 of 140 (5.7%) patients. Food allergy respiratory symptoms are, in our experience, almost always associated with other clinical manifestations (e.g., cutaneous, gastrointestinal). The recognition of food-dependent IgE-mediated respiratory symptoms is essentially limited to those cases characterized by food allergy with asthmatic expression. It is possible, however, that in many cases foods may have a nonspecific role in the determination of asthma or in the preparation of bronchi for the possible consequent stimulus.

Asthma↗
PubMed1988

Sensitivity to tomato and peanut allergens in children monosensitized to grass pollen.

Possible associations between allergy to grass pollen and positive skin tests to food allergens were studied in 102 children monosensitized (as to inhalant allergens) to grass pollen, and in 117 children monosensitized (as to inhalant allergens) to Dermatophagoides. Thirty-two foods were tested by an epicutaneous method. Positive skin tests to food allergens were more frequent in children with allergy to grass pollen (59.8%) than in children with allergy to Dermatophagoides (9.4%). A considerably high frequency of positive reactions to tomato (39.2%), peanut (22,5%), green pea (13.7%), and wheat (11.7%) was observed in children with allergy to grass pollen. Positive skin tests to peanut closely correlated with positive RAST results and nasal provocation tests, whereas in children with skin test positivity to tomato a close correlation with nasal provocation tests but a 45% correlation with a positive RAST result were observed. RAST inhibition experiments were carried out, and the results may suggest the presence of cross-reacting IgE to grass pollen, tomato, and peanut antigens. Clinical implications of these findings are discussed in the light of histories of food hypersensitivity, urticaria-angioedema, and atopic dermatitis in children with allergy to grass pollen.

Adolescent↗
PubMed1988

Occurrence and subclass distribution of IgG antibodies to dietary antigens in children with atopic dermatitis and in their mothers.

Occurrence and subclass distribution of IgG to alpha-lactalbumin (ALA), beta-lactoglobulin (BLG), and ovalbumin (OA) have been determined in 30 children with atopic dermatitis (AD) and in their mothers. Thirty nonatopic (NA) children and their mothers were studied as controls. Total IgE and IgE to ALA, BLG, OA, Dermatophagoides pteronyssinus, and Lolium perenne were also investigated. The main purpose of this study was to determine whether in humans, as observed in animals, maternal allergen-specific IgG influences allergen-specific IgE synthesis in children. IgG1, IgG2, IgG3, and IgG4 to ALA, and IgG1 and IgG2 to OA were more frequent in AD children than in NA children. As compared with mothers of NA children, mothers of AD children had similar frequency and levels of IgG subclasses to BLG and to OA, but a higher frequency of IgG1, IgG2, and IgG3 to ALA. In no case did total or allergen-specific IgE levels correlate with any subclass of allergen-specific IgG, which in turn influenced neither total IgE levels nor allergen-specific IgE distribution. The presence of allergen-specific IgG of any subclass in mothers was not associated with modifications in total IgE levels nor in allergen-specific IgG subclass distribution in AD children. But occurrence of IgE to OA was higher in AD children whose mothers had IgG4 to OA. This finding may be a model of interference in specific IgE response in the child caused by isotype response in the mother.

Adult↗
PubMed1988

Selectivity in IgG subclass response to hepatitis B vaccine in infants born to HBsAg-positive mothers.

IgG subclasses of antibodies to the hepatitis B surface antigen (HBsAg) in sera from 40 healthy infants immunized with the vaccine against hepatitis B virus (HBV) were detected using an enzyme-linked immunosorbent assay (ELISA) with monoclonal antibodies. The infants were born to asymptomatic HBsAg-positive mothers. Total serum IgG subclasses were also tested to exclude a deficiency of certain subclasses in these infants but their distribution was the one expected according to age. In contrast, IgG subclass antibodies to HBsAg were predominantly IgG1 and IgG4. The collected data indicate that infants produce significantly higher levels of IgG1 and IgG4 than IgG2 and IgG3 in response to the vaccine for HBV. The IgG4 response to anti-viral vaccinations is uncommon. The role of that IgG4 subclass is not yet clear: even if an anaphylactic role was suggested, no adverse reactions were observed in vaccinated children.

Adult↗
PubMed1986

Multiple carboxylase deficiency due to deficiency of biotinidase.

A patient with biotinidase deficiency was studied in whom the first admission to hospital for acidosis occurred at 5 years of age. Sensorineural abnormalities of the optic and auditory nerves antedated diagnosis and treatment with biotin, and these sensory losses did not resolve with treatment. The other clinical manifestations of the disease were highly responsive to biotin. Biotinidase was assayed using 14C-labeled natural substrate. The activity in the patient approximated 1% of the control level.

Amidohydrolases↗
PubMed1986

The multitransfused beta-thalassemic child: a model for the study of IgE response.

Positive skin tests to common inhalant allergens and the frequency of respiratory atopic diseases were determined in a population of multitransfused beta-thalassemic patients and in their parents and siblings. Positive skin test responses and respiratory atopic diseases were significantly less frequent in patients than in their relatives and in controls. Negative patients differed from positive patients as to the age at first transfusion, since the former had started transfusion earlier than the latter. It is speculated that in beta-thalassemic patients, allergen-specific IgG acquired early in life through transfusions suppresses the synthesis of allergen-specific IgE.

Adolescent↗