The presence of the glucocorticoid receptor in early and late passage C-6 glioma cells 2B clone and in astrocytes derived from aged mouse cerebral hemispheres has been documented by immunoblotting and/or immunofluorescence labelling. All cell types studied express the glucocorticoid receptor of molecular weight 97 KDa. In addition, in astrocytes derived from aged mouse cerebral hemispheres a smaller molecular weight polypeptide reacting with anti-glucocorticoid receptor antibody was also demonstrated. No difference in the amount of the 97 KDa glucocorticoid receptor between early and late C-6 2B cells was observed, whereas the astrocytes from aged cerebral hemispheres contained considerably reduced amounts of the glucocorticoid receptor compared to C-6 2B cells. Late passage C-6 2B cells were immunofluorescence labelled with the anti-glucocorticoid antibody, the receptor being almost exclusively present in the cytoplasm, with particular concentration in the perinuclear region. The presence of glucocorticoid receptor of molecular weight 97 KDa in glial cells corroborates and expands the existing data based on radioligand binding and immunocytochemical studies. These cell populations can be exploited as a model system for the study of the effects of glucocorticoids on senescence and brain aging.

Animals↗

PubMedDate not supplied

Urinary cortisol excretion rates and anxiety in normal 1-year-old infants.

Urinary cortisol excretion rates were determined for each voiding during 8 hours on 3 days for 20 infants. The first day served as the control. On the second day stress was imposed by having the mother leave the infant for an hour. On a third day the child was stimulated by novel toys and socialization for an hour in the mother's presence. There was no significant difference between mean cortisol excretion rates on control and stress days. Variability in cortisol levels was significantly greater on the day of stress than on the other 2 days. Ratings of separation anxiety and levels of cortisol excreted 40 to 160 min after the imposed stress were positively correlated (r = 0.46, P less than 0.01). Cortisol levels also differentiated between anxious infants who were agitated from those who were withdrawn. No altered levels of cortisol were associated with excitement.

Anxiety, Separation↗

PubMedDate not supplied

Nucleic acid content in developing rat brain after prenatal and/or neonatal exposure to high altitude.

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Adaptation, Physiological↗