Search PubMed⌕ Search

Biomedical subjects

A Verhoeff

Publications and source records attributed to A Verhoeff.

At least 37 records · Page 2Linked to original sources
PubMed1994

A randomized, comparative study of a single oral dose of fluconazole versus a single topical dose of clotrimazole in the treatment of vaginal candidosis among general practitioners and gynaecologists.

OBJECTIVES: To compare efficacy, acceptability and patient preference of a single oral dose of fluconazole with a single intravaginal dose of 500 mg clotrimazole (medication groups) in women with vaginal candidosis visiting gynaecologists or general practitioners (study groups). DESIGN: A comparative, randomized multicenter study. EVENTS: Baseline visit and treatment, short-term follow-up after 1 week and long-term follow-up after 4 weeks. At each visit, symptoms were graded and cultures were obtained. RESULTS: Symptomatic and mycological efficacy did not differ statistical significant in the medication groups or study groups. A complicated history regarding vaginal candidosis was more often found among gynaecologists, yielding poorer results of treatment. Patients preferred oral treatment over intra-vaginal treatment. CONCLUSIONS: No differences were found in the clinical and mycological efficacy of both drugs. Clinical results were related to parameters originating from the patients history, resulting in less favourable results in the study group of gynaecologists.

Administration, Intravaginal↗
PubMed1994

Effects of estrogen treatment and inhibition of prostanoid synthesis on myometrial activity and gap junction formation in the oophorectomized ewe.

The objective was to elucidate the functional relationship between estradiol-induced changes in myometrial activity, uterine prostanoid synthesis, and gap junction formation in vivo. The study design was as follows. The effects of inhibition of prostanoid synthesis with naproxen on formation of gap junctions and coordination of electrical and mechanical myometrial activity induced by estradiol-17 beta were investigated in 6 chronically instrumented oophorectomized ewes. Prostanoid metabolites were measured in arterial and uterine venous plasma. Myometrial biopsies were obtained to determine gap junction area. The results were that intraarterial administration of 0.1 mg of estradiol-17 beta was followed by a significant increase in maximum rate of rise of the intrauterine pressure cycles and a significant decrease in conduction time of bursts of electrical activity; this response was not altered by pharmacologic inhibition of uterine prostanoid synthesis using naproxen. Estradiol administration during continuous naproxen infusion increased gap junction area. The conclusion is that improved coordination of myometrial activity and formation of myometrial gap junctions induced by estradiol is not mediated by prostaglandins.

6-Ketoprostaglandin F1 alpha↗
PubMed1994

A randomized study of transcervical intrafallopian transfer of pronucleate embryos controlled by ultrasound versus intrauterine transfer of four- to eight-cell embryos.

OBJECTIVE: To establish the value of transcervical intrafallopian transfer of zygotes and the accuracy of fallopian cannulation. DESIGN: A prospective randomized study, comparing ultrasonically controlled transcervical intrafallopian transfer of zygotes with intrauterine transfer of cleaved embryos. SETTING: Department of IVF of the Rotterdam Academic Hospital. PATIENTS: One hundred forty-five patients with patent tubes entered the IVF program. MAIN OUTCOME MEASURE: Pregnancy rates in both groups and ultrasound (US) assessment during fallopian cannulation and ET. RESULTS: Transvaginal cannulation of the tube appears not to be sufficiently accurate when performed without US guidance. Catheter damage occurred in many cases. The previously reported superior implantation rate after intrafallopian transfer in comparison with intrauterine transfer could not be confirmed. CONCLUSIONS: Intrauterine transfer of cleaved embryos remains the method of choice in IVF.

Adult↗
PubMed1994

A comparison of in vitro fertilization results after embryo transfer after 2, 3, and 4 days of embryo culture.

Embryo transfer results after a 2 to 4 day period of embryo culture were compared. Two thousand two hundred ninety-seven ETs, performed in 1991 and 1992, were analyzed. Ongoing pregnancy rates after 2, 3, or 4 days of embryo culture were 23.3%, 21.9%, and 26.4%, respectively. Multiple pregnancy rates were 36.2%, 38.8%, and 32.6% per ongoing pregnancy for the three groups, respectively. The implantation rate of 73 cavitating morulae on day 4 was surprisingly high (41%) compared with that of other developmental stages. Transfer after 4 days of culture gives the ability to recognize embryos with a very high implantation potential.

Cells, Cultured↗
PubMed1993

Ovarian cysts, cytology and histology: a conflicting story.

Ovarian cysts from 27 selected patients were examined cytologically as well as histologically. The aspirates were obtained by means of ultrasound or laparoscopy. Cytological examination in 17 of the cases did not show malignancy, but histological examination demonstrated malignancy in 5 of these patients. The fluid of the other 10 cysts indicated malignancy, which was confirmed histologically in only 4 cases. From this study we conclude that cytological examination has little to offer in diagnosing the true nature of ovarian cysts.

Adult↗
PubMed1992

Comparison of results obtained with human serum and a protein solution as a supplement for in vitro fertilization culture medium.

A prospective controlled study was performed to compare the PRs obtained after use of a uniform IVF culture medium containing a pasteurized serum protein solution or patient serum. The ongoing PRs per ET in the serum and the protein solution group were 32% and 28%, respectively (not significant). Culture of supernumerary embryos showed blastocyst formation and even hatching with both supplements. The PR will not drop when this protein solution is used as a protein supplement in IVF culture medium instead of patient serum.

Blood↗
PubMed1990

Single-dose oral fluconazole versus single-dose topical miconazole for the treatment of acute vulvovaginal candidosis.

OBJECTIVES: To compare efficacy, safety and patient preference of a single oral dose of 150 mg fluconazole with a single intravaginal dose of 1200 mg miconazole in vaginal candidosis. To investigate the effect of treatment on Candida colonization of throat and rectum. DESIGN: Double-blind, double-dummy, parallel, randomized trial. Ninety-nine patients with symptomatic and mycologically verified candidosis were given 150 mg fluconazole with an intravaginal dummy, or 1200 mg miconazole with an oral dummy. Patients with an inadequate short-term response were given a second dose. RESULTS: At each visit a patient self assessment and an investigators' global assessment were recorded, and cultures were set up. Adverse events were recorded and laboratory tests were performed. Clinical cure or improvement (investigators' assessment) was obtained in 100% (short-term) and 95% (long term) of the fluconazole group and in 94% and 90%, respectively, of the miconazole group. Patients considered the treatment excellent or good in 81% (short-term) and 88% (long-term) in the fluconazole group and in 84% and 76%, respectively, of the miconazole group. Mycological cure was achieved in 98% (short-term) and 73% (long-term) of the fluconazole group and in 96% and 82% respectively in the miconazole group. The differences in results were not significant. Both treatments significantly reduced the number of positive rectal cultures: neither treatment had a significant effect on throat cultures. Four percent of the patients preferred intravaginal therapy. CONCLUSION: A single dose fluconazole is as safe and effective as a single dose of miconazole.

Acute Disease↗
PubMed1987

Effects of instrumentation on myometrial activity in the oophorectomized ewe.

The influence of an intrauterine pressure catheter on electrical myometrial activity (EMG) was investigated in chronically instrumented oophorectomized sheep. The EMG of non-catheterized (NC) ewes (n = 4) was characterized by long-lasting but infrequently occurring bursts of electrical activity, whereas the EMG of catheterized (C) ewes (n = 4) showed short and frequent bursts. Intra-arterial administration of 0.1 mg of 17 beta-estradiol (E2) was followed in C and NC ewes by an increase in myometrial activity after an initial silent period. E2 did not essentially change the pattern of long and short bursts in either group. Intravenous administration of the prostaglandin-synthesis inhibitor naproxen changed the EMG pattern in the C ewes, both before and after E2, to a pattern resembling the EMG of NC ewes. The results of this study indicate that electrical myometrial activity is markedly influenced by the presence of an intrauterine pressure catheter, probably due to enhanced prostaglandin synthesis.

Animals↗
PubMed1986

Electrical and mechanical uterine activity and gap junctions in estrogen-treated oophorectomized sheep.

The effects of a single dose (0.1 mg) of 17 beta-estradiol on myometrial electrical activity, intrauterine pressure, and myometrial gap junctions were studied in six chronically instrumented oophorectomized ewes. The intrauterine pressure cycles were analyzed for the maximum rate of rise, peak pressure, and active pressure area, together with the frequency of the cycles and the burst frequency, and related to the gap junction area. 17 beta-estradiol temporarily depressed uterine activity. The period of quiescence was followed by a pronounced increase in all variables of electrical and mechanical activity and also by a rise in gap junction area. The greatest increase in the maximum rate of rise of the intrauterine pressure cycles occurred 24 hours after estrogen and was associated with the maximum increase in gap junction area. These results indicate that a single dose of 17 beta-estradiol induces formation of myometrial gap junctions, which may facilitate the spread of electrical activity over the myometrium and improve coordination of uterine contractility.

Animals↗
PubMed1986

Myometrial activity related to gap junction area in periparturient and in ovariectomized oestrogen treated sheep.

Propagation of electrical (EMG) activity in the myometrium may be facilitated by the presence of gap junctions (GJ), leading to improved synchronization of contractility. EMG and mechanical (IUP) activity in relation to GJ area were studied in 9 periparturient and in 2 ovariectomized (OVX) oestradiol-17 beta (E2) treated ewes, chronically instrumented with bipolar electrodes and intrauterine sponge-tip catheters. Myometrial biopsies were taken under epidural anaesthesia at various time intervals around delivery and after intra-arterial administration of 0.1 mg of E2. In pregnant ewes we found a significant increase in the rate of rise and area of IUP cycles during labour. Both were closely related to a significant increase in GJ area. In E2 treated OVX sheep we found a significant increase in GJ area, with a maximum at 24 hours after injection. The increase in GJ area was associated with a significant increase in the rate of rise of IUP cycles. The results of our study support the hypothesis that gap junctions facilitate the spread of EMG activity across the myometrium, which may improve synchronization of uterine contractility during labour.

Animals↗