[Potential activity of Ca2+/Mg2+-dependent endonuclease in endometrial hyperplasia and cancer].
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Biomedical subjects
Publications and source records attributed to A V Zhdanov.
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DNA hybridization for detecting HSV, CMV, C. trachomatis, and U. urealyticum by biotin-labeled DNA probe was used for investigating clinical specimens from patients with infertility and chronic urogenital inflammations. High sensitivity and specificity of the method was confirmed by the results of PCR, ELISA, and immunofluorescent methods in 80-90% cases. DNA hybridization technique is a simple method requiring no sophisticated equipment, which recommends it for the diagnosis of sexually-transmitted diseases at clinical laboratories.
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Immunologic studies were carried out in 29 women with clinical manifestations of chronic inflammations of the internal genitals. Dot-hybridization with biotin-labeled DNA probes revealed chlamydial DNA in the cervical cells of 14 women, Herpes simplex type 2 and/or cytomegaloviral DNA in 15 women. Cell-mediated immunity was assessed using monoclonal antibodies CD3, CD4, CD8, and CD19 and by Facscan flow cytofluorometer (Becton Dickinson). Immunoglobulins were measured by nephelometry with Abbott kits and TDx device. No appreciable changes in the mean values of the parameters characterizing T-cellular immunity were revealed in women with genital inflammations and chlamydial infection (p < 0.05). The counts of CD19+ lymphocytes varied to a certain degree, and IgM levels were increased in patients with chlamydial infection, this pointing to activation of B-lymphocytes with Chlamydia. A viral infection of the genitals was associated with a reduction of T-cellular immunity parameters and an imbalance in the ratio of immunoregulatory cells at the expense of reduction of the share of lymphocytes expressing CD4 antigen.
Two microbial strains have been isolated from lake water. The strains oxidize ferrous compounds and manganese. By the structure of microcolonies and the character of deposited oxides of these metals, the strains are identical to the genus Siderocapsa. However, according to their growth cycle and some morpho-physiological characteristics, they were included into the genus Arthrobacter (Corynebacteriaceae). Since these microorganisms differ, by their cultural and morpho-physiological properties, from other species of this genus, they were classed as a new species. Arthrobacter siderocapsulatus nov. sp.
A digital memory and a control unit are described that along with the pulse X-ray TV diagnostic system enable reduction of the exposure dose in the course of X-ray examinations. Diagnostic capabilities of the system are improved due to possibility to view up four images at various examination angles at the display and to scale every image.
A wide spectrum of heterotrophic and autotrophic microorganisms was detected in the zones of decomposition of spodumene and bed rocks, pegmatites and shales, in the spodumene deposit. The following aerobic microorganisms which did not from spores predominated in the deposit: Arthrobacter globiformis, A. pascens, A. simplex, Nocardia globerula, Pseudomonas fluorescens, Ps. putida, Ps. testosteronii. The following specific bacterial groups were found: thionic, sulfate reducing, and nitrifying bacteria. Degradation of spodumene and bed rocks was found to occur in moist regions containing cracks; it was accompanied with a decrease in pH. A possible role of microorganisms in decomposition of spodumene as well as removal of elements is discussed.