Search PubMed⌕ Search

Biomedical subjects

A V Nikitin

Publications and source records attributed to A V Nikitin.

At least 73 records · Page 4Linked to original sources
PubMed1991

[Multi-factor analysis of combined effects of rifampicin and peptidoglycan of microbial origin on immune response].

Multifactorial analysis of the combined action of rifampicin and a microbial peptidoglycan on the immune response to antigens of the vaccine EV fraction 1 was performed. The results were computer-processed and the second order equations describing delayed hypersensitivity (DH) and antibody titers were derived. Nomographs or equal level curves showing interrelations of the investigated factors were plotted. The character of the combined action on DH and antibody titers was heterogeneous. The peptidoglycan had a pronounced immunostimulant action on DH and, to a lesser extent, influenced the humoral response. Conditions for the peptidoglycan use aimed at immunostimulation were optimized with application of multifactorial analysis.

Adjuvants, Immunologic↗
PubMed1991

[Multifactorial experiment on the combined action of doxycycline and a low molecular weight immunomodulator of microbial origin in experimental anthrax infection].

Chemotherapeutic efficacy of combined therapy of experimental anthrax infection with subtherapeutic doses of doxycycline and a low molecular weight immunomodulator of microbial origin was studied with mathematical design of the experiment and multifactorial analysis. A marked synergistic effect of oral doxycycline and the immunomodulator was observed. The results of the multifactorial experiment were computer processed and polynomial statistic models (the second order equations) describing the survival rate and mean lifespan (MLS) were derived. The equal level lines characterizing the survival rate and MLS were plotted against the fixed values of the time factor of administering the immunomodulator and the dose of the antibiotic. The doses of the immunomodulator and the time of its administration were optimized with respect to the maximum therapeutic effect with doxycycline subtherapeutic doses.

Adjuvants, Immunologic↗
PubMed1991

[A multifactorial analysis of the combined action of an antibiotic and a low-molecular immunomodulator of microbial origin in experimental plague infection].

The combined effect of doxycycline and a low molecular weight immunomodulator of microbial origin was studied in experimental plague infection with mathematical design of the experiment. Synergism of the action of the antibiotic and immunomodulator used in subtherapeutic doses was observed. The action was the most pronounced when the drugs were applied therapeutically. On the basis of the multifactorial analysis the regimens for the use of the antibiotic and immunomodulator were optimized.

Adjuvants, Immunologic↗
PubMed1991

[The characteristics of the immune status in patients with the Wolff-Parkinson-White syndrome].

A study is presented of 32 patients (18 men, 14 women; age from 16 to 38 years) with WPW syndrome. Immunological investigations revealed characteristic of WPW syndrome phenotypic peculiarities with characteristic changes of the immune status, allowed to evaluate the mechanism of formation of Kent's bundle and to discuss the problem of primary prophylaxis by means of immunoactive agents.

Adolescent↗
PubMed1991

[Multifactorial study of the combined effect of rifampicin and microbial peptidoglycan in experimental plague infection].

The combined effect of rifampicin and a microbial peptidoglycan was studied in multifactorial experiments on noninbred mice with plague infection. The effect of rifampicin and the immunomodulator was shown to be synergistic. The results of the multifactorial experiments provided designing of polynomial statistic models of the second order characterizing the animal survival rate and mean life-span and plotting of nomograms or equal level lines useful in optimization of the combined therapy parameters.

Animals↗
PubMed1991

[Multifactorial experiment on the combined effect of rifampicin and microbial polysaccharide in experimental plague infection].

Multifactorial analysis was applied to the study of the combined effect of rifampicin and a microbial polysaccharide in experimental plague infection. The effect of the antibiotic and immunomodulator was shown to be synergistic. On the basis of the study results polynomial statistic models of the second order were designed and nomograms or equal level lines were plotted which provided optimization of the combined chemo- and immunotherapy.

Animals↗
PubMed1991

[Comparative multifactorial analysis of combined administration of injection and peroral forms of an antibiotic with a microbial immunomodulator in experimental anthrax].

Comparative efficacy of the use of injection and oral dosage forms of rifampicin in the subtherapeutic doses in combination with peptidoglycan , an immunomodulator of microbial origin, was studied in respect to experimental anthracic infection with application of multifactorial analysis. It was shown that the antibiotic and immunomodulator had a pronounced synergistic effect. Polynomial statistic models were developed and nomograms or equal level curves defining the survival rate and average life-span (ALS) of the experimental animals within a wide range of the antibiotic and immunomodulator doses and the peptidoglycan dosing time were plotted. The combined use of the injection rifampicin in the subtherapeutic doses and the immunomodulator provided a significant increase in the survival rate and ALS, whereas the use of the oral antibiotic in combination with the immunomodulator increased only the ALS and not the survival rate. Multifactorial analysis proved to be an optimal methodical approach to comparative study of various antibiotic dosage forms used in combination with immunomodulators under experimental conditions.

Adjuvants, Immunologic↗
PubMed1991

[Optimal combined use of rifampicin and immunomodulator of microbial origin in experimental Q-Rickettsia infection].

Multifactorial analysis of the combined use of rifampicin and an immunomodulator of the microbial origin, such as peptidoglycan, was performed on a model of experimental Q fever in albino mice. On the basis of the experimental results, statistic polynomial models describing the weight of the murine spleens and the titers of the complement-binding antibodies were designed. It was shown that the action of the immunomodulator and antibiotic was highly synergistic with respect to the chemotherapeutic activity and antibody titers. The preventive use of the immunomodulator yielded a 30-fold decrease in a rifampicin therapeutic dose. The use of the immunomodulator also provided a pronounced immunomodulating effect with respect to humoral immunity. Nomographs for optimizing the dose-time parameters of the antibacterial and immunomodulating therapy were plotted.

Adjuvants, Immunologic↗
PubMed1991

[Chemotherapeutic effectiveness of doxycycline in experimental Q fever].

Comparative chemotherapeutic efficacy of doxycycline administered orally or parenterally was studied on a model of experimental Q fever in guinea pigs and albino mice. The advantage of the parenteral use of the antibiotic was observed only in the treatment of the albino mice. Optimization of the doses and time of doxycycline use providing the maximum therapeutic effect and the required levels of the complement binding antibodies was achieved with employing the multifactorial analysis.

Administration, Oral↗
PubMed1991

[Immunomodulating effect of levamisole in antibiotic therapy].

The use of ampicillin, benzylpenicillin, gentamicin, erythromycin, cefamezine or cephaloridine in combination with levamisole aimed at increasing their efficacy and correcting the adverse reactions was studied. Immunological activity of the antibiotics was comparatively characterized by using standard methods. It was shown that the character of the antibiotic effect on the basic factors of nonspecific resistance and immunity was heterogeneous. Schemes for using the antibiotics in combination with levamisole for correcting the detected disorders in the immunity status of the experimental animals were developed.

Adjuvants, Immunologic↗
PubMed1990

[Treatment of patients with corticoid-dependent bronchial asthma by step-by-step intra-pulmonary administration of glucocorticoids].

The efficacy of a local step-by-step intrapulmonary and systemic administration of glucocorticosteroids (GCS) in 61 patients with corticoid dependent bronchial asthma was studied. A significantly earlier normalization of the basic clinical symptoms of exacerbation of the disease and an improvement of the external respiration function were revealed in the main group of patients, as compared to the controls. The method of a local step-by-step intrapulmonary administration of GCS made it possible to increase the treatment efficacy by bringing down the GCS dosage required to maintain++ remission and excluding complications caused during intrapulmonary injection of drugs to patients with corticoid dependent bronchial asthma.

Adult↗
PubMed1990

[Multifactor analysis of the combined action of doxycycline and a peptidoglycan of microbial origin on the immune response].

Multifactorial analysis of the combined action of a microbial peptidoglycan and doxycycline on the immune response to antigens of the vaccine EV fraction 1 was made. Nomograms or equal level curves characterizing delayed hypersensitivity (DH) and antibody titers in various doses of the peptidoglycan and the antibiotic were plotted by the experimental data with a computer. The peptidoglycan had a pronounced immunomodulatory action on DH and antibody titers. However, the types of regulation of the both responses markedly differed. With multifactor analysis, the range of the values of the operating parameters, i.e. the drug doses and the time of their administration providing the required levels of DH and antibodies under the conditions of the combined therapy were defined.

Animals↗
PubMed1990

[Multifactor analysis of the combined use of an antibiotic and a low molecular weight immunomodulator of microbial origin in experimental plague infection].

Multifactorial analysis of the combined effect of rifampicin and a low molecular immunomodulator of microbial origin in experimental plague infection was performed. Synergism of the antibiotic used in the subtherapeutic doses and the immunomodulator was shown. By the results of the study polynomial statistic models of the second order describing the survival rate and average life-span of the experimental animals were developed and nomographs (equal level curves) were plotted for rapid estimating the therapy quantitative parameters. Optimization of the combined use of rifampicin and the immunomodulator on the basis of the multifactorial analysis was achieved.

Adjuvants, Immunologic↗
PubMed1990

[The efficacy of piracetam in treating patients with chronic bronchitis and concomitant atherosclerosis].

The nootropic agent pyracetam was used in patients suffering of chronic bronchitis associated with concomitant atherosclerosis. Pyracetam was administered intravenously in saline solutions. It was found that the group of patients receiving pyracetam revealed earlier normalization of clinico-laboratory indices. Pyracetam also produced immunocorrective and antioxidant effect resulting in normalization immunity values and activity of lipid peroxidation.

Aged↗
PubMed1990

[Mg2+-dependent doxycycline-induced inhibition of leukocyte adhesion].

The method for estimating in vitro leukocyte adhesion was modified in relation to investigation of the effect of antibiotics and other pharmacological agents on cell activation. The adhesion intensity was estimated in standard 96-cavity microplates from the content of beta-hexosaminidase in the adherent cells. That made it possible to easily automatize the method by using the equipment for enzyme immunoassay. It was shown that spontaneous and induced adhesion of guinea pig leukocytes depended on temperature and bivalent ions. The level of spontaneous adhesion mainly depended on Mg ions. Doxycycline, 0.1 to 100 micrograms/ml, did not induce degranulation of leukocytes and had no cytotoxic action. Doxycycline in supratherapeutic concentrations (greater than 10 micrograms/ml) inhibited the spontaneous leukocyte adhesion. In special experiments it was found that the doxycycline's inhibitory effect was partially reversible and associated with ++chelation of Mg ions rather than Ca ones.

Animals↗
PubMed1990

The effect of sodium nucleinate on allergic and immunological reactions.

Experiments on immunized rabbits and guinea pigs indicated that sodium nucleinate (SN) was capable of weakening or entirely eliminating anaphylactic and skin reactions of delayed type hypersensitivity to repeated administration of staphyloanatoxin, APDT vaccine. The findings on patients with the infectious form of bronchial asthma and chronic rheumatism showed that sodium nucleinate attenuated reactions to the subcutaneous administration of staphylococcal and streptococcal allergens. The treatment of patients suffering from infectious-allergic bronchial asthma and rheumatism with SN resulted in the recovery of deficient T cells, T-suppressors, normalization of immunoglobulin concentrations. In children with acute glomerulonephritis sodium nucleinate normalized decreased T-suppressor cells and increased IgG and circulating immune complexes (CIC), resulting in a pronounced remission of disease. The mechanism of desensitization and elimination of CIC by SN has not been explored, however, the parameters of SN-induced immunomodulation are known rather completely. It is suggested that SN brings about accumulation in the cell of cyclic AMP which diminished membrane permeability, activates monoaminooxidase resulting in the degradation of histamine and other biogenic amines, enhances the synthesis of endogenous corticosteroids with their desensitizing properties. All these effects contribute to the elimination of delayed type hypersensitivity. The role of SN in the inhibition of delayed type hypersensitivity remains obscure.

Animals↗