Biomedical subjects

A V Antelava

Publications and source records attributed to A V Antelava.

5 recordsLinked to original sources

PubMed2006

[Oxidative stress during thyrotoxicosis and its correction].

Oligocrine is a preparation of vegetable origin characterized by anti-inflammatory, antioxidant, immunomodulating properties. Our study shows that administration of L-thyroxine injections (irrespective of the duration) results in elevated synthesis of thyroid hormones and intensification of oxidative stress in experimental animals. These changes are evidenced by accumulation of membrane phospholipide peroxidation products - lipid peroxides in the blood. At that we should point that while dependence of FT3 and FT4 levels on the duration of L-thyroxine administration was insignificant in the experimental model of thyrotoxicosis, intensity of lipid peroxidation increased along with prolongation of injections. Blood levels of free nitric oxide were decreased likely due to the transformation of nitric oxide into peroxinitrite. Oligocrine facilitates to the stabilization of thyroid status and restriction of NO hyperproduction, hypermetabolism and oxidative stress in the body.

Animals↗

PubMed2003

Effect of richlocaine alone or in combination with energostim on the severity of endotoxemia and survival of the skin under conditions of reduced blood flow.

The local anesthetic richlocaine decreased the area of necrosis in the skin flap under conditions of reduced blood flow by 29.5%. Improved survival of skin flap after richlocaine treatment alleviated endogenous intoxication, reduced secondary inflammatory reaction, improved liver function, and normalized the ratio between vasoconstricting and vasodilating prostaglandins. This effect was most pronounced after combination therapy with richlocaine and direct-action antihypoxant energostim.

Alanine Transaminase↗

PubMedDate not supplied

[Effect of energostim on myocardial damage during coronary artery occlusion].

In contrast to traditional therapy (beta-adrenoblocker and nitrates), energostim improves the systolic and diastolic functions of myocardium during 120-min occlusion of the left descending coronary artery. The energostim-induced improvement in the central hemodynamics is correlated with an adaptive increase in activity of the antioxidant system enzymes in response to the ischemic production of reactive oxygen species, which is evidence of the mobilization of reserves of the enzymatic link in the antioxidant defense system of cardiomyocytes. Analogous pattern is observed in the blood. In the control group of traditional therapy, a decrease in the superoxide dismutase (SOD) activity and the redox potential (NAD/NADH) in myocardium are correlated with a decrease in the maximum rate of pressure increase in the left ventricle (R 6.4, p < 0.01) observed 2 h after the coronary occlusion. In the energostim treated group, there is a correlation between the SOD activity and the content of cytochrome C in mitochondria (R 6.1, p < 0.01): a change in the level of cytochrome C during 2-h acute ischemia is correlated with the decrease in redox potential (NAD/NADH) and in the ratio of glutathione peroxidase to Mn-dependent SOD (r 0.64, p < 0.01). Thus, disturbances in the antioxidant defense system of both myocardium and blood plasma of the patients with acute myocardium infarction are correlated with inability of the energy supply system to utilize oxygen in the process of glycolysis and oxidative phosphorylation in mitochondria. Stable adaptive increase in activity of the antioxidant defense system enzymes and a decrease in the content of cytochrome C in the blood plasma are probably the independent indications of beneficial prognosis and high efficacy of the proposed treatment of the ischemic damage of myocardium.

Animals↗

PubMedDate not supplied

[Cardioprotective effect of energostim in toxic allergic myocarditis].

Energostim is a combined drug comprising a mixture of nicotinamide adenine dinucleotide (0.5 mg), cytochrome C (10 mg), and inosine (80 mg), representing antihypoxant and antioxidant of direct action in one ampule. After pretreatment and subsequent 3-day energostim therapy of animals with 3-day toxico-allergic myocarditis (3d-TAM), the ECG was free of any rhythm disorders and showed evidence of improved conduction, restoration of the normal form of T-wave and the position of ST segment, while the content of myofibrillar fraction of creatine phosphokinase and toxic products of disturbed metabolism (degree of endotoxemia) decreased to the upper normal level. Under the action of energostim, neither pressure nor the maximum rate of pressure buildup in the left ventricle are reduced (as they do upon 3d-TAM); neither systolic and diastolic functions are disturbed, nor their coordination (r = 0.79 between dP/dtmin and dP/dtmax, p < 0.01). The restoration of contractile activity and maximum rate of relaxation of myocardial microfibrils during 3d-TAM is accompanied by an increase in the content of adenyl nucleotides, in the ATP/ADP, ADP/AMP, NAD/NADH, and NADP/NADPH ratios, and in the cytosol phosphorylation potential. The energostim-induced improvement in the energy supply system are accompanied by restoration of the ability of sarcoplasmic reticulum to efflux Ca2+. Thus, it is demonstrated that the effect of energostim is related to its ability to actively participate in intracell metabolic processes in myocardium, abolish necrotic changes and endotoxicosis, and restore homeostasis in the systems responsible for the contraction--relaxation process (thus preventing from the development of dysfunction of the left ventricle and the heart failure).

Animals↗

PubMedDate not supplied

[Thyroid proteinase activity in toxic forms of goiter].

Shifts of the thyroid proteinase (cathepsin) activity in thyrotoxicosis were studied. It was found that both free and total activity of the thyroid lysosome cathepsins increase. The free activity, expressed as a percentage of the total one, is maximum in diffuse toxic goiter and is minimum in the healthy thyroid tissue. These differences in the thyroid cathepsin activity are due to destabilization of the lysosome membranes and their increased number in thyrotoxicosis according to the electron microscopy data. It is suggested that the thyroid lysosome cathepsins play a leading part in organic proteolysis, stipulating the thyroid hormone release.

Cathepsins↗