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Biomedical subjects

A Thiele

Publications and source records attributed to A Thiele.

46 records · Page 3Linked to original sources
PubMed1993

Density heterogeneities of hepatitis C virus in human sera due to the binding of beta-lipoproteins and immunoglobulins.

Heterogeneities in the density of hepatitis C virus RNA-carrying material (HCV-RNA-CM) found in human sera (1.03-1.20 g/cm3) are attributed to the binding of low-density lipoproteins and/or of IgG. In some sera HCV-RNA-CM seems to be nearly totally bound to beta-lipoproteins and cannot be precipitated by anti-IgG (gamma); in others more than 95% of HCV-RNA-CM is bound to IgG and cannot be precipitated by anti-beta-lipoprotein. Furthermore, there are sera from which HCV-RNA-CM can be completely be precipitated by either anti-beta-lipoprotein or anti-IgG (gamma), pointing to a binding of the two serum proteins to the same HCV-RNA-CM. There are other sera from which HCV-RNA-CM can be partially precipitated by the one or the other antiserum, leaving behind fractions, which are bound to beta-lipoprotein or to IgG. HCV-RNA-CM cannot be precipitated from some sera either by anti-beta-lipoprotein or by anti-IgG (gamma).

Centrifugation, Density Gradient↗
PubMed1993

DNA typing with digoxigenin-11-dideoxyuridinetriphosphate-labelled oligonucleotide probes enables non-radioactive analysis using a dual detection system: application for screening HLA-DQA1 polymorphisms.

We describe a standardized, highly sensitive, nonradioactive detection procedure for HLA class-II typing of DQA1 alleles and suballeles which has important and cost-effective application in studying HLA disease associations. The procedure involves polymerase chain reaction-based target DNA sequence amplification and dot blotting followed by stringent hybridization with digoxigenin-11-dideoxyuridine triphosphate 3'-end-labelled allele-specific oligonucleotide probes. The dual detection system described here makes this procedure very versatile.

Alleles↗
PubMed1991

Pattern of retinotectal projection in the megachiropteran bat Rousettus aegyptiacus.

The retinotopic organisation of the superior colliculus (SC) in the megachiropteran bat Rousettus aegyptiacus was examined with single and multi-unit recordings and by tracing the retrograde and anterograde transport of horseradish peroxidase (HRP) between the retina and the SC. The pattern of projection of the visual field onto the SC in Rousettus resembles the pattern found in most mammals. The whole of the contralateral visual field is represented and, in addition, a region of the ipsilateral visual field extending 25 degrees beyond the vertical 0 degree meridian. The ipsilateral visual field is represented binocularly in the most anterior 300-500 microns of the rostral pole of the SC. The contralateral visual field up to 25 degrees from the vertical meridian is represented through both eyes for the next 500-800 microns. The peripheral part of the contralateral visual field, 25 degrees-110 degrees from the vertical meridian is seen only by the nasal retina (the monocular crescent) of the contralateral eye and is represented in the caudal part of the SC. Following multiple injections of HRP into one SC, ganglion cells were labeled in both the nasal and temporal hemiretina of the contralateral eye. In the retina ipsilateral to the injection site, labeled cells were restricted to the temporal hemiretina. After injections of HRP into one eye, labeled terminals were found all over the contralateral SC, but in the ipsilateral SC they were restricted to a band that begins 300-500 microns caudal from the rostral pole and extends to the middle of the SC. These results suggest that in Rousettus, unlike the megachiropteran bats described by Pettigrew, Jamieson, Robson, Hall, McAnally, and Cooper (Philosophical Transactions of the Royal Society of London Series B 325:489-559, 1989), the retinotopic organisation of the SC is not primate-like, but follows the general mammalian scheme. As the retinotopic organisation of the SC is not consistent among the megachiropteran bats, the pattern of this projection may not be a useful indicator of their phylogenetic origins.

Animals↗
PubMed1989

Potent leukocidal action of Escherichia coli hemolysin mediated by permeabilization of target cell membranes.

The contribution of Escherichia coli hemolysin (ECH) to bacterial virulence has been considered mainly in context with its hemolytic properties. We here report that this prevalent bacterial cytolysin is the most potent leukocidin known to date. Very low concentrations (approximately 1 ng/ml) of ECH evoke membrane permeability defects in PMN (2-10 x 10(6) cells/ml) leading to an efflux of cellular ATP and influx of propidium iodide. The attacked cells do not appear to repair the membrane lesions. Human serum albumin, high density and low density lipoprotein, and IgG together protect erythrocytes and platelets against attack by even high doses (5-25 micrograms/ml) of ECH. In contrast, PMN are still permeabilized by ECH at low doses (50-250 ng/ml) in the presence of these plasma inactivators. Thus, PMN become preferred targets for attack by ECH in human blood and protein-rich body fluids. Kinetic studies demonstrate that membrane permeabilization is a rapid process, ATP-release commencing within seconds after application of toxin to leukocytes. It is estimated that membrane permeabilization ensues upon binding of approximately 300 molecules ECH/PMN. This process is paralleled by granule exocytosis, and by loss of phagocytic killing capacity of the cells. The recognition that ECH directly counteracts a major immune defence mechanism of the human organism through its attack on granulocytes under physiological conditions sheds new light on its possible role and potential importance as a virulence factor of E. coli.

Adenosine Triphosphate↗
PubMed1985

[Differential analysis of the binding forms of iron in various plant foodstuffs].

In fourteen plant foodstuffs of various composition a differentiation of iron contents according to water soluble, in ethylacetate extractable, in labile contents and iron(II) or Fe(III) species in the water extract are carried out by the spectrophotometric Ferrozine method. High water-soluble labile iron parts and high iron(II) contents were found in fruit, high complexed parts in the water extract and ethylacetate extractable iron contents in protein rich foodstuffs. The iron total contents are situated between 1 and 90 ppm, the water soluble parts amount to 6 up to 60% of the total content according to the foodstuffs.

Acetates↗
PubMedDate not supplied

[Anti-Jo-1 antibodies: specific autoantibodies for polymyositis with interstitial pulmonary fibrosis. 2 case reports].

Anti-Jo-1 antibodies are rare autoantibodies, which bind and inhibit the activity of histidyl-tRNA-synthetase. They are predominantly found in a genetically and clinically distinct subset of myositis patients, presenting with interstitial alveolitis. We describe the case of a 22-year-old woman with Jo-1-syndrome with typical features of myositis, pulmonary fibrosis, nonerosive symmetric polyarthritis, Gottron's papules at the metacarpophalangeal joints and classic heliotrope discoloration of the periorbital area with edema. The patient did not respond to chloroquine, azathioprine, intravenous immunoglobulins or cyclophosphamide. Remission was finally achieved with oral methotrexate plus corticosteroids. The second case describes a 34-year-old man with myositis, who rapidly developed respiratory insufficiency after suffering from joint pain and stiffness for about 10 weeks. He responded well to cyclophosphamide pulse therapy and high-dose corticosteroids. The second case demonstrates that control of the interstitial alveolitis is most important for long-term outcome. Therefore, immunosuppressive drugs should be used along with corticosteroids as early as possible.

Adrenal Cortex Hormones↗