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A Termijtelen

Publications and source records attributed to A Termijtelen.

At least 55 records · Page 3Linked to original sources
PubMed1984

Ninth International Histocompatibility pre-workshop testing of Dw6 HTCs. Two subtypes of Dw6.

In the scope of the cellular part of the 9th International Histocompatibility Workshop, the offered homozygous typing cells (HTCs) of several specificities have been screened in a pre-Workshop. In Leiden and Seattle all HTCs typing for "HLA-Dw6" have been tested. This implied that in both laboratories mixed lymphocyte culture (MLC) matrices were performed between the Dw6 HTCs and that all HTCs were tested as stimulator cells against a panel of heterozygous responder cells. The results clearly demonstrated that "HLA-Dw6" as defined by the different participating laboratories can be split into two major groups, Dw6.a and Dw6.b. This observation confirms and extends previous reports. None of the 9th Workshop B-cell sera could discriminate between the two presently described subgroups of HLA-Dw6.

HLA-DR Antigens↗
PubMed1983

Mapping SB in relation to HLA and GLO1 using cells from first-cousin marriage offspring.

A newly defined leukocyte antigen system, the secondary B-cell (SB) system, was shown to be linked to HLA. The SB marker has been investigated in lymphocyte donors presumed to be genetically homozygous for HLA-A through HLA-D/DR by virtue of descent from a first-cousin marriage and of phenotypic homozygosity for these HLA markers. Of 19 donors, 3 were found to be heterozygous for SB. Studies of the families of these three donors could not distinguish with certainty whether the heterozygosity resulted from SB/DR recombination or from "pseudohomozygosity" for HLA-A through -D/DR by inheritance of two genetically unrelated but similar haplotypes. However, our data favored the occurrence of SB/DR recombination with a meiotic distance perhaps as large as 3.3 cMorgan. Recombinations were identified which mapped SB between HLA-B and GLO1. These studies demonstrate the usefulness of cells from first-cousin marriage offspring in mapping a polymorphic genetic system.

B-Lymphocytes↗
PubMed1983

LB-Q1 and LB-Q2: two determinants defined in the primed lymphocyte test and independent of HLA-D/DR, MB/LB-E, or SB.

The primed lymphocyte test (PLT) was used to prepare reagents between HLA-D/DR identical individuals. Two sets of primed lymphocytes were obtained which recognized the new determinants referred to as LB-Q1 and LB-Q2, respectively. LB-Q2 is significantly associated with HLA-A1, -B8, and -D/DR3. Nevertheless, LB-Q1 and LB-Q2 seem to be distinct from any of the established HLA antigens. No association with any of the alleles of the SB system was observed. A recombination between HLA-D/DR and HLA-B suggested that the locus encoding LB-Q2 is situated on the telomeric side of HLA-D/DR.

Epitopes↗
PubMed1983

Reanalysis of the HLA-DRw6 complex.

The serological definition of the HLA-DRw6 specificity has been complicated by the lack of monospecific reagents. During the 8th International Histocompatibility Workshop several subspecificities of DRw6 were proposed. In the present study we have reanalysed the reactions of 8th Workshop and local sera in families and homozygous typing cells. These studies have revealed four DRw6 related reaction patterns. The patterns are described and compared with those reported by others. Two of these specificities are commonly observed in Dutch Caucasoids, one related with Dw6 and one with Dw9. The other DRw6 related reaction patterns are very infrequent in this population. In addition, the relation between a locally defined specificity LB5x8 and two new specificities 8wDRw13 and 8wDRw17 is discussed.

Adult↗
PubMed1982

Recognition of a polymorphic monocyte antigen in HLA.

The serological recognition of 'new' gene products of the HLA system can be accomplished by selecting sera with strong leucocyte antibodies and testing these against a panel of cells obtained from donors which are compatible for the known HLA antigens with the antibody producer. This approach, which we termed the HLA-CAP approach (compatible with antibody producer), was essential in the recognition of what is now called the HLA-DR locus and of non HLA-linked T-cell subgroups. Using the same technique, we have defined here several sera recognizing a structure on monocytes similar to one of the alleles of the HLA-linked PL3 or secondary B-cell (SB) system, so far only recognized by cellular techniques.

Adult↗
PubMed1982

Cell membrane polymorphisms coded for in the HLA-D/DR region. I. Relation between D and DR.

The relation of HLA-D and -DR determinants was studied in Dutch Caucasoids. The recognition of subgroups of DR4, DR5, and DR7, and the specificities LB12 and LB13 are described. Phenotype and gene frequencies and a Hardy--Weinberg analysis of DR and local (LB) B-cell groups are given. Excellent correlation between D and DR typing was obtained when HTCs were studied by selected B-cell antisera. When the same sera were used to type a panel of D typed cells, the correlation was decreased (with the exception of DR3 and Dw3). In the case of discrepancies the DR specificity, but not the corresponding D specificity, always could be found and not the other way around. The data fit best the assumption that HLA-D and -DR are carried by the same molecule, although they might be different determinants on this molecule. A number of possible explanations for the observed discrepancies has been given.

Cell Membrane↗
PubMed1981

The role of primary MLC of the non HLA-D/DR determinant PL3A.

To test the influence in primary MLC of the newly PLT defined determinant PL3A (Termijtelen et al. 1980), 11 Dw3/DR3 homozygous individuals were tested in an MLC matrix. Two groups of mutually negative cells were detailed. The results appeared to correlate with the presence of PL3A. Cells from group 2 were stimulated by cells from group 1, but the reverse reactions were negative. A genetic model for this "one way" stimulation, observed in MLC as well as PLT, is discussed. We suggest that PL3A, which causes strong stimulation in PLT, also plays a role in primary MLC.

Epitopes↗
PubMed1981

Complexity of stimulation in MLC and the influence of matching for HLA-A and -B.

The influence of HLA-A and -B on primary mixed lymphocyte cultures (MLCs) was investigated in homozygous typing cell (HTC) experiments and by testing HLA-D/DR-identical, heterozygous individuals. Compatibility or identity for HLA-B between HLA-D-compatible or -identical responder and stimulator combinations resulted in lower MLC reactivity in many cases. Since this was especially true for haplotypes containing antigens with strong linkage disequilibrium, it was suggested that this observation was due to matching for antigens encoded by the B-D region rather than to matching for HLA-B itself. An additional influence of matching for HLA-A could not be demonstrated, possibly owing to the fact that stimulation caused by the HLA-A region is too weak to influence our results. The complexity of stimulation in MLC is discussed.

HLA Antigens↗
PubMed1980

DR typing in Australian Aborigines. An indication for a second locus in the HLA--D region defined by serology.

A group of 43 Australian Aborigines was typed for HLA--DR determinants and other antigens on B cells, using pregnancy sera from Caucasoid women. The group could not be considered as random, since a selection was made for an equal number of Au antigen positive and Au antigen negative individuals The typing results showed that the specificities DRw2, 4 and 8 were as well defined in this population as in Caucasoids, whereas the specificities DRw1, 3, 5 and 7 could not be defined. Even more interesting was the finding that a cluster of six sera, defining the local B cell specificity LB12, gave positive reactions in 41 out of 43 Aborigines. Although LB12 is found to be highly associated with DRw1, 2 and 6 in the Caucasoid population, in 36 cases the LB12 positive Aborigines could not be demonstrated to carry the specificities DRw1 or 2. The recognition of DRw6 is difficult at the moment and for the reason its presence or absence in this material is uncertain. However, three individuals typed positive for DRw4 and 8 and for LB12 as well. These findings strongly support the hypothesis that the genes coding for LB12 and its postulated allele LB13 are separate from the genes coding for the known DR determinants. LB12 is very likely identical to the recently described determinants DC1 (Tosi & Tanigaki 1979) and MB1 (Duquesnoy et al. 1979a).

Antilymphocyte Serum↗
PubMed1980

A new determinant, defined by PLT, coded for in the HLA region and apparently independent of the HLA-D and DR loci.

A PLT cell was raised between a responder cell which carried the HLA-D and DR specificities Dw3, 8; DRw3, 8 and a stimulator cell which was most likely homozygous for HLA-Dw3, DRw3. The PLT cell appeared to recognize a determinant (PL3A) which was (1) different from the officially recognized HLA-D and DR determinants, (2) was associated with HLA-A1, B8, Dw3 and DRw3, and (3) segregated in three informative families with HLA. Another responder-stimulator combination could be selected to raise PLT cells which recognized the same determinant.

Chromosome Mapping↗
PubMed1977

Evidence for two series of B-cell antigens in man and their comparison with HLA-D.

Population and family studies of five sera with antibodies against B-cell determinants show an excellent fit of some of them with the HLA-D determinants, suggesting that the HLA-D determinants themselves or closely linked structures can be recognized by serology. One serum, Ag, might be coded for by a locus different from HLA-D, which could be located between HLA-A and -B. A two-color fluorescence test to detect B-cell antigens was used and found to save time and reagents and to give very good results.

B-Lymphocytes↗
PubMed1977

Cellology of HLA. I. The apparent homogeneity of HLA-D.

We have demonstrated that there is a similarity in the distribution of restimulation responses of primed lymphocyte typing (PLT) cells raised between related and unrelated individuals, thus leading to the suggestion that HLA-D is a homogeneous determinant. It is proposed that within HLA a hitherto unrecognized heterogeneous structure may exist that is closely associated with HLA-D and which may give rise to intermediate reactions in primary mixed lymphocyte reactions and PLT.

Epitopes↗