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Biomedical subjects

A Tashiro

Publications and source records attributed to A Tashiro.

89 records · Page 5Linked to original sources
PubMed1977

pH and the activity of porphobilinogen synthase in human blood.

For determination of the activity of this enzyme (EC 4.2.1.24) in blood, pH dependency was evaluated by using whole blood and washed erythrocytes. The effects of assay methods, original and final pH, and use of sodium or potassium phosphate buffer were examined. The pattern of pH dependency differed for whole blood and washed erythrocytes, whether blood from normal or lead-exposed subjects was examined. The pH optimum for the activity in erythrocytes from lead-exposed subjects was the same as for the normal persons if the concentration of lead in blood did not exceed 80 microgram/100 g of whole blood. Hemolyzing blood by placing it on solid CO2 decreases the activity.

Erythrocytes↗
PubMed1976

Size and structure of the cholera toxin molecule and its subunits.

Cholera toxin (choleragen) dissociated into two types of subunit with molecular weights estimated to be 28,000 daltons (A) and 11,000 daltons (B); this dissociation was effected by gel filtration at acid pH with or without urea. Subunit A could be separated into two fragments, A1 (23,000 daltons) and A2 (about 2,500 daltons), after reduction and alkylation. Choleragenoid (68,000 daltons) appeared to be a polymerized form of subunit B. A-specific antigen was found in choleragen as well as in A1 and A2, while B-specific antigen was found in both choleragen and choleragenoid. In electron micrographs, the toxin molecule appeared as a particle of uniform size consisting of subunits in V- or Y-shape and in ring form. The dimensions coincided with the values calculated from the molecular weights.

Antigens, Bacterial↗