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Biomedical subjects

A Tashiro

Publications and source records attributed to A Tashiro.

At least 55 records · Page 3Linked to original sources
PubMed1990

Histological changes in cardiac hemochromatosis improved by an iron-chelating agent. A biopsy case.

Cardiac dysfunction and ECG abnormalities were demonstrated in a 51-year-old woman suffering from secondary hemochromatosis in sideroblastic anemia. Hemosiderin deposition in vacuolized and disarrayed myocytes was disclosed by microscopic examination of the first biopsy specimen of endomyocardial tissue. Three months after administration of deferoxamine mesylate, an iron-chelating agent, the clinical findings were improved. The second endomyocardial biopsy revealed marked depletion of hemosiderin deposition in the myocytes, and improvement of myocyte vacuolization and disarray. Ultrastructurally, the highly electron-dense granules in the myocytes were also decreased in number and density. X-ray microanalysis revealed a prominent peak of Fe in the granules. In a liver specimen obtained by needle biopsy 5 months after the second endomyocardial biopsy, marked hemosiderin deposition still remained.

Biopsy↗
PubMed1990

Relation between myocardial histological changes and ventricular tachycardia in cardiomyopathy: a study by 24-hour ECG-monitoring and endomyocardial biopsy.

The relation between myocardial histological changes and ventricular tachycardia (VT) in cardiomyopathy was investigated. Right ventricular endomyocardial biopsy and 24-hour ECG-monitoring were performed in 19 patients with dilated cardiomyopathy (DCM) and 22 with hypertropic cardiomyopathy (HCM). Cardiomyopathy was histologically divided into the following four groups: group A, hypertrophy without disarray of myocytes (3 DCM and 7 HCM); group B, hypertrophy with disarray of myocytes (14 HCM); group C, fibrosis (9 DCM and 1 HCM); and group D, diffuse myocytic degeneration (7 DCM). VT was observed in 20% (2 of 10 patients) of group A, 14% (2 of 14) of group B, 80% (8 of 10) of group C, and 71% (5 of 7) of group D. The degenerating myocytes and/or the irregular distribution of fibrosis may play an important role in the etiology of VT in cardiomyopathy.

Adult↗
PubMed1990

[Glomerulocystic kidney: report of two autopsy cases].

We reported two cases of glomerulocystic kidney (GCK). Case 1 was a 35-day-old male who was born at 38 weeks of gestation and weighed 1750 g at birth. He showed chromosomal aberration and surface anomaly, and was diagnosed as having pentalogy of Fallot. There were no signs of renal disturbance. At autopsy, subcapsular cysts composed of dilated Bowman's capsules were found. Case 2 was a 63-year-old man. He suffered from mild cerebral infarction at the age of 45. At the age of 51, he was diagnosed as having chronic glomerulonephritis and began hemodialysis at the age of 55. He died of uremic lung at 63. Diffuse dilatation of Bowman's capsules were found in the renal cortices. In the liver, proliferation and cystic dilatation of interlobular bile ducts were noted. The patients with GCK without any severe disease in other organs show no or stable symptoms in the early period of life, but later developed severe renal insufficiency.

Dilatation, Pathologic↗
PubMed1989

Antimyristoylation of the gag proteins in the human immunodeficiency virus-infected cells with N-myristoyl glycinal diethylacetal resulted in inhibition of virus production.

The gag proteins of human retroviruses such as human immunodeficiency virus (HIV-1) are specifically myristoylated in their amino termini (1, 2, 3). N-myristoyl glycinal diethylacetal (N-Myr-GOA) and other N-Myr-compounds (N-Myr-Gly-GOA, N-Myr-Gly-Gly-GOA and N-Myr-Gly-Gly-Gly-GOA) were newly synthesized and investigated for activity of antimyristoylation of these gag proteins and for influence on viral replication. Of the N-Myr-compounds tested, N-Myr-GOA most severely inhibited the protein myristoylation; N-Myr-Gly-GOA also inhibited it, but moderately. Furthermore, it was observed that N-Myr-GOA at 20 microM caused noticeable inhibition (about 80%) of the production of mature HIV in the HIV-1-infected MT-4 cells. In this system, N-Myr-GOA substantially inhibited more than 90% of the N-myristoylation of p17 gag protein produced in the HIV-1-infected MT-4 cells. These results suggest that the N-myristoylation of p17 gag protein of HIV-1 may be essential in its structural assembly or maturation.

Acetaldehyde↗
PubMed1989

Antibodies to an NH2-terminal myristoyl glycine moiety can detect NH2-terminal myristoylated proteins in the retrovirus-infected cells.

Novel antibodies were raised against a synthetic NH2-terminal myristoyl glycine moiety which is characteristic of N-myristoyl-proteins. Antisera raised against N-myristoyl-Gly-hemocyanin reacted with N-myristoyl-Gly-[125I]albumin. The immunoreaction was competed for by albumin conjugated with N-myristoyl-glycine, while underivatized albumin had no effect. Of the [3H]myristate-labeled proteins detected, pp60v-src, which is a transforming protein of Rous sarcoma virus, and p19gag and p17gag, which are core proteins in the human T-cell leukemia virus and the human immunodeficiency virus, were identified as N-myristoylated proteins by the radioimmunoprecipitation analyses with the antibody.

Amino Acid Sequence↗
PubMed1989

[A new edentulous model maintained at body temperature].

We have produced a new edentulous model, which is maintained at the human body temperature, for higher grade model practice and pre-clinical education. A soft material, its thickness based on the value of the oral mucosa, covers the surface of this model. It takes 4 minutes to reach the body temperature after turning on the switch, and it consumed about 9 watts per hour. This new model is useful for teaching clinical characteristics of impression materials in which the setting time is influenced by the temperature. Improvements are under consideration to fit some thermal and pressure sensors to the buccal and labial parts to make a more sophisticated patient simulator.

Body Temperature↗
PubMed1988

Prevention of horizontal transmission of hepatitis B: efficacy of hepatitis B immunoglobulin and vaccine in an institution for the handicapped.

In a Japanese institution for the handicapped with confirmed continuous outbreaks of hepatitis B virus (HBV) infection by horizontal nosocomial transmission, 29 susceptible subjects (8 institutionalized children and 21 medical staff) were injected intramuscularly with anti-human HBs immunoglobulin (HB Ig) and subcutaneously with HB vaccine. All cases acquired HBs antibody after injection of HB Ig and seropositivity for HB after the third inoculation of HB vaccine was 78.6%. No new case of HB occurred among the study population throughout the period investigated. This suggested the effectiveness of HB Ig and HB vaccine in the prevention of horizontal nosocomial transmission of HBV.

Adolescent↗
PubMed1988

Antimyristoylation of gag proteins in human T-cell leukemia and human immunodeficiency viruses with N-myristoyl glycinal diethylacetal.

N-Myristoyl (N-Myr-) glycinal (aminoacetoaldehyde, GO) diethylacetal (A), which is abbreviated as N-Myr-GOA, and other N-Myr-compounds (N-Myr-Gly-GOA, N-Myr-Gly-Gly-GOA, and N-Myr-Gly-Gly-Gly-GOA) were newly synthesized and then employed for NH2-terminal antimyristoylation of structure proteins in the human T-cell leukemia virus (HTLV-I) and the human immunodeficiency virus (HIV). The protein myristoylation of structure proteins p19gag, of HTLV-I, and p17gag, of HIV, was determined separately, using radiolabeled myristic acid, in vitro. The radiolabeled proteins, after immunoprecipitation with an antiserum to adult T-cell leukemia (ATL) or the anti-p17gag monoclonal antibody of HIV, were identified as p19gag of HTLV-I and p17gag of HIV by fluorography after SDS-polyacrylamide gel electrophoresis (SDS-PAGE). The protein myristoylation was resistant to NH2OH-treatment. Of the N-Myr-compounds tested, N-Myr-GOA remarkably prevented the myristoylation of p19gag and p17gag, but N-Myr-Gly-Gly-GOA and N-Myr-Gly-Gly-Gly-GOA did not.

Acetaldehyde↗
PubMed1987

Acute pancreatitis with cytomegalovirus infection.

An autopsy case of necrotizing pancreatitis with cytomegalovirus (CMV) infection is reported. A 50-year-old housewife was admitted because of malignant choroid plexus papilloma of the fourth ventricle. Serum amylase transiently elevated during irradiation and chemotherapy for brain tumor, one month before death. The patient died of a respiratory failure. At autopsy focal necrosis was mainly observed in the peripancreatic adipose tissue and occasionally in the parenchyma of the tail. Fibrosis and inflammatory infiltration was found around the necrosis and interstitium. Cytomegalic cells with intranuclear inclusions and/or cytoplasmic granules were observed frequently in the acini near the necrosis. Immunohistological study revealed CMV antigen in the cytomegalic cells. Herpesvirus-type particles were observed in the nucleus and cytoplasm of the cytomegalic cells. CMV infection of the acini are thought to cause necrotic changes in the pancreas.

Acute Disease↗
PubMed1987

Cervical catecholamine-secreting paraganglioma in the pterygopalatine fossa.

A 25-year-old man was admitted for examination to determine the cause of hypertension. High levels of noradrenaline in plasma and urine were seen, suggesting that the patient had an adrenal or extra-adrenal pheochromocytoma. However, whole-body scintigraphy using the isotope of [131I] meta-iodo-benzylguanidine (131I-MIBG) failed to find the presence of a catecholamine-secreting tumor. The highest level of noradrenaline was detected in plasma obtained from the left jugular vein after selective venous collections through femoral catheterization. Both the computed tomography and cerebral angiography showed a hen-egg-sized tumor located in the left pterygopalatine. After surgical removal of the tumor, high blood pressure and the levels of noradrenaline in plasma and urine were significantly decreased. Histopathological diagnosis was paraganglioma (catecholamine-secreting paraganglioma). The patient with cervical catecholamine-secreting paraganglioma is the first case where the tumor was isolated and located in the pterygopalatine fossa.

3-Iodobenzylguanidine↗
PubMed1986

Effects of chaetoglobosin J on the G-F transformation of actin.

It was shown that substoichiometric concentrations of chaetoglobosin J, one of the fungal metabolites belonging to cytochalasins, inhibited the elongation at the barbed end of an actin filament. Stoichiometric concentrations of chaetoglobosin J decreased both the rate and the extent of actin polymerization in the presence of 75 mM KCl, 0.2 mM ATP and 10 mM Tris-HCl buffer at pH 8.0 and 25 degrees C. In contrast, stoichiometric concentrations of cytochalasin D accelerated actin polymerization. Chaetoglobosin J slowly depolymerized F-actin to G-actin until an equilibrium was reached. Analyses by a number of different methods showed the increase of monomer concentration at equilibrium to depend on chaetoglobosin J concentrations. F-actin under the influence of stoichiometric concentrations of chaetoglobosin J only slightly activated the Mg2+-enhanced ATPase activity of myosin at low ionic strength. It is suggested that when the structure of the chaetoglobosin-affected actin filaments is modified, the equilibrium is shifted to the monomer side, and the interaction with myosin is weakened.

Actins↗
PubMed1986

Production of stable mouse x human hybridomas secreting HBs antigen-specific human monoclonal antibody by using in vitro sensitization.

The stable mouse x human hybridomas were established by cell fusion of a mouse myeloma cell line, a P3-X63-Ag8-653 (P3-653) subclone, with human peripheral blood mononuclear cells (PBMs) from normal volunteers for production of human monoclonal antibody against a predefined, clinically important antigen of viral origin, hepatitis-B surface (HBs) antigen. For successful mouse x human fusions, it was extremely important to preselect volunteers and to sensitize their PBMs by using in vitro sensitization. The resulting mouse x human hybridomas have continuously secreted a relatively high amount of human monoclonal antibodies to HBs antigen over nine months without cloning.

Animals↗
PubMed1986

Significant regression of the tumor combined with the high level of plasma cortisol by low-dose administration of O, p'-DDD in a case with Cushing's syndrome caused by adrenocortical carcinoma.

The effect of o, p'-DDD on a functioning adrenocortical carcinoma is described. A 52-year-old woman, who had a huge adrenocortical carcinoma and pulmonary metastasis, was treated by a low-dose administration of o, p'-DDD, which reduced the tumor size. The excretion of urinary 17-OHCS was decreased, whereas plasma cortisol was not decreased but rather increased. This was considered to be caused that o, p'-DDD functioned as a cytotoxic factor which did not improve the states of hyperkalemia, hyperglycemia and hypertension. This suggested that monitoring to measure multiple steroids in addition to plasma cortisol and urinary 17-OHCS was useful for recognizing the general conditions of patients with adrenocortical carcinoma receiving o, p'-DDD therapy.

Adrenal Cortex Neoplasms↗
PubMed1986

Analysis of defective delayed-type hypersensitivity in autoimmune mice bearing lpr gene.

Many functional defects of T-cells have been found in autoimmune prone, MRL/MpJ-lpr/lpr (MRL/lpr) mice. We examined the capacity of delayed-type hypersensitivity (DTH) induction and macrophage migration inhibition factor (MIF) production. MRL/lpr and C57BL/6J-lpr/lpr (B6/lpr) mice were tested for capacity of DTH by the footpad reaction to purified protein derivative of tuberculin (PPD) and lung granuloma formation after immunization with BCG-cell wall (BCGCW). In lpr mice, the ability to induce DTH against exogeneous antigenic stimulation decreased with age. The results show that the defect of DTH in lpr mice caused by deficiency of the MIF producing capacity of proliferated T-cells rather than suppressor cell activity for MIF production.

Aging↗