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Biomedical subjects

A Takeda

Publications and source records attributed to A Takeda.

At least 523 records · Page 29Linked to original sources
PubMed1983

Secretory component and IgA in endometrial adenocarcinomas. An immunohistochemical study.

The localization of secretory component (SC) and IgA was immunohistochemically studied in 6 normal endometrium and 55 endometrial adenocarcinomas including 34 well, 11 moderately and 10 poorly differentiated ones. In normal endometrium, SC localization was found in the cytoplasm of epithelial cells and luminal contents of the gland. IgA showed similar localization of SC. Secretory phase endometrium contained proportionally larger numbers of positive cells for SC and IgA than proliferative phase endometrium. SC localization was found in all cases of well and moderately differentiated carcinomas, while it was found only in 4 cases out of 10 poorly differentiated carcinomas. IgA localization was similar to that of SC and this condition was thought to reveal the binding of IgA to SC existing in the tumor cells. The present immunohistochemical study revealed that the staining intensity of SC well correlated with the histological grade of differentiation of the tumors.

Adenocarcinoma↗
PubMed1983

Influence of single doses of dothiepin and amitriptyline on physiological measures and psychomotor performance in normal young and elderly volunteers.

The subjects were 14 (7 young and 7 elderly) men. Self-rating, and physiological and psychological measurements were applied, and experiments were performed 24 h after drug administrations. 25 mg dothiepin hydrochloride (DP) decreased critical flicker frequency less than 25 mg AMP in both age groups. 25 mg DP reduced whole mouth salivary rates less than 25 mg AMP, especially in the young subjects. 25 mg DP decreased diastolic blood pressure more than 25 mg AMP. Systolic blood pressure (after 25 mg DP and 25 mg AMP) and whole mouth salivary rates (after 25 mg AMP) were affected more intensely in the elderly than in the young volunteers.

Adult↗
PubMed1982

Polyembryoma of ovary producing alpha-fetoprotein and HCG: immunoperoxidase and electron microscopic study.

Polyembryoma of ovary producing alpha-fetoprotein (AFP) and human chorionic gonadotropin (HCG) was studied by indirect immunoperoxidase method for AFP and HCG, and electron microscopy. Clinically, this patient showed pseudoprecocious puberty caused by elevated HCG which is synthesized by trophoblastic cells in polyembryoma. She is put under VAC (vincristine, actinomycin-D, cyclophosphamide) chemotherapy after operation and shows no signs of recurrence including reelevation of serum AFP at five months after operation. Embryoid bodies which we studied correspond to normal embryo at 15- or 16-day stage. Immunoperoxidase study showed that AFP is synthesized by yolk sac cells of the embryoid bodies and HCG is synthesized by syncytiotrophoblastic cells. The finding about AFP synthesis suggests that normal embryo at 15- or 16-day stage may begin AFP synthesis. Electron microscopic study showed that each part of the embryoid bodies had some characteristic structures. Most striking features found in the cytodifferentiation of the embryoid bodies were noticed in some special differentiation of plasma membrane and existence of surface coat. Desmosomes were found in endodermal cells and yolk sac cells. Ectodermal cells were attached to each other by zonulae occludentes and adherentes. Microvilli were found in ectodermal cells and yolk sac cells. Two different kinds of surface coat were found in mesodermal cells and lining cells of yolk sac cavity: thin-layered deposit of electron-dense material covering the plasma membrane facing intercellular space of mesodermal cells and endodermal cells, and thick-layered deposit of electron-dense material which covered the plasma membrane facing yolk sac cavity of endodermal cells and yolk sac cells. Presence of similar characteristic material in RER of yolk sac cells led us to speculate that thick deposit was synthesized by yolk sac cells and secreted into yolk sac cavity. Combined with immunoperoxidase study by light microscopy, we assume that this thick-layered deposit has some close relation to AFP.

Child↗
PubMed1982

Role of serum in inhibition of cultured lymphocytes by lysophosphatidylcholine.

Serum was heated at various temperatures to inactivate components which might be involved in the regulation of lysophosphatidylcholine (lysoPC) levels in rabbit lymph-node cell cultures. Cells cultured in medium containing serum preheated for 20 min at 66 degrees C ("66 degrees C-serum") were inhibited much more by exogenous lysoPC (5 microM) than were cells cultured in medium containing control serum ("38 degrees C-serum"). This was observed over a 20 h culture period as a slow increase in inhibition of cell labelling with [3H] uridine, which reflected cytotoxic cell damage. Heating serum at 66 degrees C caused (i) conversion of monomeric albumin to highly polymeric forms which were deficient in lysoPC-binding activity, (ii) transfer of lysoPC from albumin to lipoproteins, predominantly high density lipoproteins, and (iii) inhibition of two lysoPC metabolizing activities (which were detected only at low levels in control serum). Addition of albumin to cultures containing 66 degrees C-serum decreased the toxicity of lysoPC to the same extent as did the addition of control serum with an equivalent albumin content. Thus, albumin was the major heat-labile factor protecting cells against lysoPC. However, cell inhibition by lysoPC was dependent on the sequence of heating serum and lysoPC addition. Inhibition was small when lysoPC was added before heating the serum. This could not be explained by a detectable difference in the binding of lysoPC to serum components. Furthermore, although radioactive labelling of cells with [14C] lysoPC was increased in 66 degrees C-serum, this did not correlate with cell inhibition. Increased labelling with [14C] lysoPC occurred several hours before significant cell inhibition was evident and was not affected by the sequence of heating and lysoPC addition. Since preincubation of lysoPC with 66 degrees C-serum increased the inhibition, it is suggested that the heated serum lysoPC generates another factor which is responsible for the cytotoxic effects observed.

Animals↗
PubMed1982

A radioreceptor assay for insulin: direct measurement of human serum insulin.

A simple radioreceptor assay was established with sensitivity and accuracy sufficient to detect concentrations of insulin in human peripheral sera. The radioreceptor assay was carried out at 4 degrees C using rabbit erythrocyte membranes and 125I-insulin. In the absence of native insulin, approximately 31% of the total 125I-insulin concentration was bound to erythrocyte membranes. Fourty-six per cent of this binding was displaced by 312 microunits/ml of native insulin. The precision expressed as coefficient of variation was 3 to 21% of the mean for the within-assay, and 1 to 21% for the between-assay, respectively. The recovery of standard porcine insulin added to the serum was 105% on the average. Insulin values in 24 samples of human sera measured by the present assay agreed well with immunoreactive insulin values.

Animals↗
PubMed1982

Formation of erythrocyte rouleaux in preheated normal serum: roles of albumin polymers and lysophosphatidylcholine.

The role of humoral factors in cell-cell interactions was studied in a simple model system: the aggregation of erythrocytes into cylindrical rouleaux when suspended in normal serum preheated at 62 degree C for 20 min. The rouleaugenic activity of heated serum was associated with an increased concentration of albumin polymers. On heating above 62 degree C, albumin released ligands, such as lysophosphatidylcholine, in quantities sufficient to convert erythrocytes to acanthocytes. The latter did not participate in rouleaux formation. Thus normal serum only became rouleaugenic when heated over a narrow range of temperatures. These properties of serum were reproduced in a system consisting only of erythrocytes, heated albumin, and lysophosphatidylcholine. Rouleau formation increased as albumin polymer size increased. Unheated normal serum could also be made rouleaugenic merely by concentrating to above normal physiological concentrations. Unheated, unconcentrated, sera from patients with various diseases are known to be rouleaugenic, but polymeric albumin appears infrequently in such sera; usually there are increases in macroglobulins are large polymeric forms of smaller serum proteins. Current evidence is consistent with the hypothesis that a small shift in the concentration of one or more of these macromolecules above a critical value promotes a phase separation of erythrocytes which spontaneously aggregate to form rouleaux.

Blood↗
PubMed1982

Studies of the function of natural killer-interferon system in patients with Sjögren syndrome.

The natural killer (NK)-interferon (IFN) system is shown to be significantly involved in the resistance of host to viral infections and to tumours in numbers of animal models (1-4). The patients with Sjögren syndrome (SS) as well as those with collagen diseases were systematically investigated for the functions of NK-IFN system, including endogenous and augmented NK activity, IFN production, and responsiveness of NK cells to IFN stimulation, using virus persistently infected cells (heLa-measles cells) as target and stimulator cells. Although endogenous NK activity was not reduced, augmented NK activity by HeLa-measles cells in vitro was significantly depressed in patients with SS compared with that in age-matched normal controls. The patients with SS had also impaired capacity to produce IFN, which is shown to be a major factor regulating NK activity (5,6) in response to HeLa-measles cells in vitro. In three patients with SS who showed severely depressed NK activity, the effect of exogenous IFN was examined, and virtually no augmentation of NK activity was observed in all cases. Under the same condition, the normal controls demonstrated a dramatic increase in NK activity. The reduced IFN production was observed in all examined patients with SS, whereas impaired augmentation of NK activity by the stimulation with HeLa-measles cells as well as IFN seemed to be more striking in patients with the systemic manifestations of the disease, such as hypergammaglobulinemia and lymphoid hyperplasia. The possible involvement of dysfunction of NK-IFN system in the systemic manifestations of SS is discussed.

Adult↗
PubMed1982

High uptake of 14C-labeled D-amino acids by various tumors.

As an extension of the previous finding that radioactivity of 14C-labeled D-amino acids after injection is localized preferentially in the tumor and the pancreas of tumor-bearing animals as compared with the corresponding L-amino acids tested, the results of similar uptake experiments using other tumors araa reported here. The present studies show high radioactivity uptake by human colon cancer, human thyroid cancer, and human leiomyosarcoma transplanted into nude mice, and by solid leukemia L1210 and solid sarcoma 180, but not by Morris hepatoma 7316A or 3'-methyl-4-(dimethylamino)azobenzene-induced rat hepatoma. The results suggest the potential utility of 11C-labeled D-amino acids for the detection of some cancers.

Absorption↗