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Biomedical subjects

A Takano

Publications and source records attributed to A Takano.

At least 73 records · Page 4Linked to original sources
PubMed1987

Immunohistochemical detection of the placental form of glutathione S-transferase in dysplastic and neoplastic human uterine cervix lesions.

Expression of the human placental form of glutathione S-transferase (GST-pi) in dysplasia (53 cases), carcinoma in situ (10 cases), and invasive carcinoma (46 cases) of human uterine cervix was investigated immunohistochemically with specific anti-GST-pi rabbit antibody. While normal squamous epithelium was largely negative, the binding of antibody was appreciable in mild and moderate dysplasias, especially in the cytoplasm of cells demonstrating koilocytotic atypia. In severe dysplasia, the nuclei as well as the cytoplasm were strongly stained in all cell layers except for the superficial layer, and in carcinoma in situ both of them were also strongly stained in all cell layers. In invasive carcinoma, over 90% of cases exhibited strong cytoplasmic staining and in over 70% the nuclei were positive. GST activity towards 1-chloro-2,4-dinitrobenzene and GST-pi protein content were significantly increased in all of 4 squamous cell carcinomas examined as compared to values for normal cervical epithelia. Two-dimensional gel electrophoresis followed by immunoblotting using the GST-pi antibody demonstrated that, of many cytoplasmic proteins, only the GST-pi subunit was specifically bound. These results indicate that GST-pi is a potentially useful immunohistochemical marker for (pre)neoplasia of human uterine cervix. In addition, it was demonstrated that the cells in severe dysplasia, carcinoma in situ, and invasive carcinoma expressing GST-pi were often characterized by staining with a monoclonal antibody to the v-H-ras gene product.

Female↗
PubMed1987

Modulation of brain progestin and glucocorticoid receptors by unsaturated fatty acid and phospholipid.

In an attempt to learn how nonsteroidal factors modulate brain progestin and glucocorticoid receptors, the effects of saturated and unsaturated fatty acids, and phosphatidylinositol on the binding of [3H]R5020 or [3H]dexamethasone, determined by sucrose density gradient and gel filtration on LH20, were examined in the cerebral cortical cytosol from 10-day-old female rats which contain a considerable amount of progestin and glucocorticoid receptors. Unsaturated fatty acids such as oleic (C18:1), arachidonic (C20:4) and docosahexaenoic acid (C22:4) depressed the [3H]R5020 or [3H]dexamethasone binding in increasing order, but saturated fatty acids had no effect. Arachidonic and docosahexaenoic acids, which were strong inhibitors, lowered the binding dose dependently. The fatty acid inhibition on brain progestin and glucocorticoid receptors was thus a function of acid dose and degree of acid unsaturation. Interestingly, prostaglandin D2 did not show any effect. Among phospholipids tested the inhibitory effect of phosphatidylinositol on the [3H]R5020 binding was evident, but no significant effect was found with phosphatidylethanolamine, phosphatidylcholine, phosphatidylserine or sphingomyelin. The phosphatidylinositol inhibition was dose dependent. Analysis on kinetics and Scatchard plot have revealed the noncompetitive type of inhibition by arachidonic acid and phosphatidylinositol. From these results it is suggested that the unsaturated nonestrified fatty acid, arachidonic acid, and phosphoinositides modulate the brain progestin and, possibly, glucocorticoid receptors through their binding at sites different from steroid binding sites on the respective receptor molecules.

Animals↗
PubMed1987

[Clinico-pathological analysis of stage IIIa carcinoma of the cervix uteri].

We have reviewed cervical carcinoma IIIa cases treated at the University of Hirosaki Hospital from 1970 to 1984 and at the Metropolitan Komagome Hospital from 1975 to 1984. In this period, 32 cases were staged at IIIa, and this group accounts for less than 2% of all cervical cancer cases at these two institutions. The age of the 32 patients ranged from 33 to 86 years with a mean age of 66. In treatment methods, 3 cases were operated on and other cases were irradiated by a combination of intracavitary therapy and external beam therapy. The actuarial 5-year survival rate was 78.3%, and the relative 5-year survival rate was 86%. Our results suggest that FIGO stage IIIa carcinoma of the cervix has a much better prognosis than FIGO stage IIIb cervical cancer.

Adult↗
PubMed1986

Inhibition of the binding of R-5020 and rat uterine progesterone receptors by long chain fatty acids.

Long chain fatty acids were known to interfere with the binding between rat uterine estrogen receptors and estradiol. The effect of long chain fatty acids on the binding between rat progesterone receptors and 3H-R5020 was studied. The binding was inhibited by palmitic acid, palmitooleic acid, arachidonic acid and docosahexaenoic acid. Docosahexaenoic acid was the strongest inhibitor and palmitic acid was the weakest inhibitor. The inhibitory effect of palmitic acid and arachidonic acid was dose dependent. In rat uterine cytosols, there existed an arachidonic acid binding factor which was distinct from progesterone receptor. The inhibitory mechanisms of long chain fatty acids was not clear, but the inhibitory effect was stronger if the number of carbon atoms increased with the number of double bonds.

Animals↗
PubMed1985

Type and screen system for elective surgery in obstetrics and gynecology.

In June, 1983, a preoperative blood ordering policy of "type and screen" was introduced in our department for elective surgery. Using this system, sera are preoperatively tested for unexpected antibodies and ABO/Rh typing is done. If the antibody screen result is negative, crossmatching is not done preoperatively. This paper reports our experience with the type and screen system in obstetrical and gynecological practice, and documents its safety, usefulness and efficiency. By adopting this system, the overall ratio of crossmatch to transfusion (C/T)-declined from 8.96 to 1.84. There were 3 patients in this series of 80 (3.75%) with a positive antibody screen result. No untoward reactions were observed following transfusion. Based on these results, the type and screen system for preoperative blood ordering is recommended for most of routine obstetrical and gynecological procedures.

Blood Group Antigens↗
PubMed1984

The morphogenesis of the vacuolation of liver cells.

The morphogenesis of the vacuolation of liver cells caused by congestion was studied in the rat. Congestion of the liver was produced by constriction of the inferior vena cava just below the diaphragm. Vacuoles appeared within 5 min after the constriction (inferior vena cava pressure: 50 mm H2O or more). Vacuolation began as a small and saccular invagination of the sinusoidal surface of the liver cell membrane, and at the initial stage the vacuolar lumen connected with the space of Disse through a narrow slit. The small vacuoles increased in size and became larger by fusing with each other. The limiting membrane of vacuoles showed identical enzymological activities to those in the liver cell membrane. Vacuoles contained some components of blood and horseradish peroxidase injected into the femoral vein. These findings suggest that vacuolation of liver cells is formed by invaginations of liver cell membrane.

Animals↗
PubMed1984

Gynecological malignancies and disseminated intravascular coagulation.

The importance of DIC in the management of gynecological malignancy, especially in advanced cases, was stressed on the basis of our experience with 8 cases. The overall incidence of DIC in our series of 366 cases of gynecological malignancies was 2.2%. However, DIC was experienced only in cases of advanced later stages and the recurrence of malignancy, and the incidence of DIC in these two groups were 2.2% and 7.2% respectively. In our 8 cases, DIC seemed to be provoked or worsened by antitumoric chemotherapy, major surgery, radiation therapy, infection, cerebral infarction and massive packed red cell transfusion. At the same time, an exceptional case of fulminating DIC which had probably been provoked by packed red cell transfusion in a patient with advanced but not detected endometrial cancer which developed in a endometriotic uterus was presented.

Adult↗
PubMed1982

Mass survey of gastric cancer and leukemia in Miyagi Prefecture, Japan.

A little more than 20,000 examinees of x-ray mass survey of gastric cancer and the controls were followed up from 1960 to 1977. Cumulative doses of x-rays were calculated for each examinee, and a collective dose in person-year-rads was constructed. Incidence of leukemia was ascertained from Miyagi Prefectural Cancer Registry. Seven cases of leukemia were found out of 242,689 person-year-rads in the irradiated population, and 9 cases out of 273,344 person-years in the controls. Incidence rate in the two groups was identical. Therefore, the risk of this mass survey was disproved.

Adult↗