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Biomedical subjects

A T Iakovlev

Publications and source records attributed to A T Iakovlev.

11 recordsLinked to original sources
PubMed1996

[Recovery rate in chemotherapy of glanders].

It was shown that out of all the studied methods for the estimation of the recovery rate in animals with experimental malleus the solid-phase enzyme immunoassay with parallel growing of the test material and subsequent isolation of the antigens and L-forms proved to be the most efficient. In addition to the routine criteria of the recovery the malleinic test was a valuable diagnostic means for the estimation of the recovery rate and the efficacy of the antibacterial drugs in a model of guinea pigs. The immunological investigations revealed a dependence of the host sanation on the term of the animal chemotherapy. There was detected no L-transformation of Pseudomonas mallei during the treatment of the animals with experimental malleus with combinations of sulfanilamides with trimethoprim (biseptol and sulfaton) or rifampicin which was evident of their high sanation activity and safety in comparison to sulfamonomethoxin used alone.

Animals↗
PubMed1988

[Pili (fimbriae) of the causative agents of plague and pseudotuberculosis: their detection in the hemagglutination reaction, the passive hemagglutination reaction and in an immunoenzyme analysis system].

Newly developed serological methods for the detection of pili in the passive hemagglutination (PHA) test with the use of immunoglobulin erythrocytic diagnosticum and in the enzyme immunoassay (EIA) with the use of specific immunoglobulins labeled with horse radish peroxidase have been found to exceed the method of detecting pili, based on the determination of their hemagglutinating activity, in sensitivity and specificity. Besides, the PHA test and EIA have proved to be capable of detecting low molecular fragments of pili, obtained by sonication and having lost their hemagglutinating activity.

Antigens, Bacterial↗
PubMedDate not supplied

[The immunodiagnosis of melioidosis by a solid-phase variant of radioimmunological analysis (RIA)].

A variant of the solid-phase radioimmunoassay (RIA) has been developed for the detection of specific immunoglobulins in laboratory animals infected with Pseudomonas pseudomallei. The proposed method has advantages over the indirect hemagglutination test and the enzyme immunoassay in its sensitivity and specificity. The newly developed RIA variant, based on group-specific antigens 6 + d, makes it possible to classify the strain causing the disease with the Asian or Australian serovar of P. pseudomallei according to the composition of detected immunoglobulins.

Animals↗
PubMedDate not supplied

[The epidemiology of cholera in Derbent District, Dagestan. The routes of the importation and spread of cholera].

The epidemic of cholera in the Derbent District of the Daghestan was imported into the region. 172 cholera patients and 204 Vibrio carriers were registered in 23 settlements of the region during the period of June 26 to October 12, 1994. The wide spread of this infection was facilitated by high migration activity of the population and the belated introduction of measures for limiting migration. The most severe outbreaks of cholera were observed in Derbent and in the villages of Mamedkala and Morskoe.

Carrier State↗
PubMedDate not supplied

[The epidemiology of cholera in Derbent District. The characteristics of the epidemic process].

Cases of cholera in the Derbent District of the Daghestan took predominantly mild and moderate clinical forms. The ratio of patients to Vibrio carriers was 1:1.25. The etiological agent of the epidemic was V. eltor, serovar Ogawa. Cholera patients were of greater epidemiological importance as the sources of infection in comparison with Vibrio carriers. Carrier state after convalescence was observed in 1.1% of cases. The population was infected with cholera, as a rule, through everyday contacts and in more rare cases by the water route. The highest rate of contamination with V. cholerae was registered in the age group of 0-2 years.

Acute Disease↗
PubMedDate not supplied

[The early laboratory diagnosis of the pulmonary form of glanders and melioidosis by using rapid methods of immunochemical analysis].

The problems of the sanitary guarding of frontiers comprise such aspects as the early recognition of infectious diseases, even those which are considered exotic in the Russian Federation. These diseases include glanders and melioidosis, infections which are endemic for some frontier regions. The study performed on experimental models of the pulmonary form of glanders and melioidosis with the acute course of the diseases demonstrates that the use of such laboratory diagnostic methods as immunosorbent variants of the enzyme-linked, chemiluminescent and immunofluorescent immunoassays permits making reliable diagnosis as early as 3-12 hours after aspiration infection.

Acute Disease↗
PubMedDate not supplied

[Pseudomonas pseudomallei and Pseudomonas mallei--capsule-forming bacteria].

The specific features of the submicroscopic structure of the causative agents of melioidosis and glanders, grown in culture media and at the initial stages of experimental infection in guinea pigs and golden hamsters, were studied with the use of electronic cytochemistry and immunocytochemistry. P. pseudomallei and P. mallei were found to be capsule-forming bacteria. According to the data obtained with the use of electronic cytochemistry, the basis of the capsular substance P. pseudomallei and P. mallei is formed by polysaccharide biopolymers protecting these bacteria from phagocytosis.

Animals↗