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Biomedical subjects

A Stenderup

Publications and source records attributed to A Stenderup.

At least 37 records · Page 2Linked to original sources
PubMed1983

Mecillinam, a new prophylactic for travellers' diarrhoea. A prospective double-blind study in tourists travelling to Egypt and the Far East.

The efficacy of mecillinam when given orally in preventing travellers' diarrhoea has been studied. Preliminary investigations in volunteers showed that single daily doses of up to 200 mg for 3 weeks were well tolerated. A randomised double-blind study was carried out in a group of tourists visiting Egypt and the Far East. The subjects took either mecillinam (200 mg daily) or placebo for 25 days. 19/36 tourists (53%) taking placebo and 5/38 tourists (13%) taking mecillinam developed travellers' diarrhoea during the treatment period (p less than 0.001). In the placebo group enterotoxigenic Escherichia coli (LT/ST) were found in 2 cases of diarrhoea and Salmonella in another 2 cases. In the remaining cases of diarrhoea the etiological agent was not found in either of the 2 groups.

Administration, Oral↗
PubMed1983

IgA and IgG serum antibodies to Candida albicans in women of child-bearing age.

IgA and IgG antibodies against Candida albicans culture filtrate were determined by enzyme-linked immunosorbent assay (ELISA) in sera from 84 pregnant and 21 non-pregnant women. IgA Candida antibody levels were found not to change significantly with age, whereas IgG Candida antibody levels were significantly higher in women aged 27 to 35 years than in women less than 24 years of age. Neither IgA nor IgG Candida antibody levels were related to the number of pregnancies. In a subgroup of 45 pregnant women examined for yeasts in the oral, anal and vaginal flora IgA Candida antibody levels were significantly higher in 13 women with negative cultures than in 19 women with recovery of C. albicans on plates. Intermediate levels of IgA Candida antibodies were found in 9 women with negative plate cultures, but positive enrichment broth cultures of C. albicans. All of 15 women with IgA ELISA values less than or equal to 0.51 had yeast-positive cultures compared with 17 of 30 women with IgA ELISA values greater than 0.51. This may suggest a role of IgA antibodies in the clearance of C. albicans from mucosal surfaces in healthy individuals.

Adolescent↗
PubMed1982

Serological classification of conjugative plasmids by indirect haemagglutination.

The indirect haemagglutination reaction was evaluated in the classification of conjugative plasmids. A simple and sensitive method was worked out using pili and in some cases whole bacteria as antigens. Antibodies were prepared against pili coded for by plasmids from incompatibility groups IncFI, IncFII, IncI alpha and IncN. The antisera were tested against pili from 35 strains harbouring plasmids. The test differentiated clearly between plasmids from unrelated incompatibility groups, whereas cross-reaction occurred with closely related groups such as FI and FII. Minor antigenic variation could be seen within the IncFII group.

Antibodies, Bacterial↗
PubMed1982

Serum antibodies to aspergillus fumigatus in patients with pulmonary aspergillosis detected by immunofluorescence.

Aspergillus antibodies of the IgG, IgA and IgM class were determined by the indirect immunofluorescence (IIF) technique in sera from 11 patients with proven pulmonary aspergillosis (group I), 11 patients with suspected pulmonary aspergillosis (group II), 34 patients with chronic obstructive lung disease (group III), 29 patients with lung infiltrates of non-fungal aetiology (group IV), and 94 blood donors (group V). The antigen was cryostate sections of formaldehyde fixed mycelium. IgG antibody titres above 640 occurred in 73% in group I + II, 12% in group III, 0% in group IV, and 22% in group V. The difference between group I + II and group V was statistically significant (p less than 0.001). IgA antibody titres above 80 were only found in patients in group I + II, suggesting that demonstration of such antibodies may be of diagnostic significance. IgM antibodies in titres between 10 and 160 were found in 100-65% in group I to V. Precipitating antibodies to a culture filtrate antigen occurred in all subjects in group I, in 9 (82%) in group II, and in 1-7% of subjects in group III to V. However, precipitin titres above 2 occurred only in group I and II. No correlation between precipitin titres and IIF titres of IgG and IgA antibodies was obtained, suggesting that the two methods reveal antibodies of different specificities.

Adult↗
PubMed1982

An IncN plasmid exhibiting a high frequency of transfer in broth media.

A mutant of the IncN plasmid N3T was isolated. This mutant (pNJ101) exhibits equal transfer frequencies in broth and on agar. IncN plasmids have rigid pili and do generally transfer at much lower frequencies in broth than on agar, hypothetically explained as the vulnerability of the pili towards convection forces in liquid. Strains with the mutant do have rigid pili, so other mechanisms regulating IncN plasmid transfer must be sought.

Conjugation, Genetic↗
PubMed1982

Isolation of Cryptococcus neoformans from pigeon manure on two media inducing pigment formation.

A newly described medium with esculin for identification of Cryptococcus neoformans was compared with Staib's Guizotia abyssinica extract-creatinine medium (GAEC) with and without diphenyl (DF). Twenty-seven samples of pigeon manure were examined. Cr. neoformans was found in 6 samples (22%) on GAEC plates (-DF); ESC medium (-DF) and malt extract agar allowed isolation from 2 and 3 samples respectively. Cr. neoformans was found in 0 to 2 samples when DF was added. Colonies of Cr. neoformans found on ESC plates had no distinctive pigmentation although inocula of pure cultures produced brown colonies. On GAEC plates some colonies of Cr. neoformans turned brown not until after 2 weeks of incubation. At 1 month the presence of pigmented colonies on GAEC plates (-DF) allowed the identification of 5 of the 6 samples from which Cr. neoformans was isolated. Other yeasts were grown from 26 samples (96%) and Torulopsis candida was found to be more frequent than Cr. neoformans.

Agar↗
PubMed1980

Cryptococcal meningitis: complications due to intrathecal treatment.

A 50-year-old woman with cryptococcal meningitis was treated with amphotericin B intrathecally through a Rickham reservoir and intravenously, together with flucytosine orally. After 4 months of treatment cryptococci and cryptococcal antigen were still present in cerebrospinal fluid from time to time. After removal of the Rickham reservoir the patient recovered completely within 6 weeks. The persistent infection was thus found to be due to the presence of the Rickham reservoir, a complication to intrathecal therapy which has not been reported before.

Amphotericin B↗
PubMed1977

Changing patterns of plasmid-mediated drug resistance during tetracycline therapy.

The patterns of drug resistance and the frequency of conjugative R plasmids in intestinal Escherichia coli from 88 patients treated for a skin disease (acne vulgaris) with low oral doses of tetracycline are reported. The proportion of patients with resistant bacteria was progressively greater in patients who received tetracycline for 1 week, 4 weeks, or longer (from 50 to 88%). No multiply drug-resistant bacteria were detected before treatment or after 1 week of treatment. After more than 4 weeks of treatment, multiply drug-resistant E. coli were isolated from about 50% of the patients. The origin and selection of R plasmid-determined multiple drug resistance are discussed.

Acne Vulgaris↗
PubMed1976

Extrachromosomal deoxyribonucleic acid in R factor-harboring Enterobacteriaceae.

Extrachromosomal deoxyribonucleic acid (DNA) from 24 different R factor-harboring Enterobacteriaceae was isolated and characterized by analytical ultracentrifugation and electron microscopy. The R factors represented 15 different patterns of transferable drug resistance found in enterobacteria from an enclosed geographic area. All of the strains contained extrachromosomal, circular DNA molecules within the range of 0.4 to 52 mum. More than one size class of circular DNA molecules was observed in the majority of the extrachromosomal DNA preparations. The buoyant density of the extrachromosomal DNA ranged from 1.700 to 1.720 g/cm3. The majority of the bacteria contained extrachromosomal DNAs of various densities. Three-fourths of the R factors were classified as fi+. The investigation illustrates the extensive variability in the physical characteristics of plasmid DNA from R factor-harboring strains.

DNA, Bacterial↗
PubMed1976

Transferable and non-transferable drug resistance in enteric bacteria from hospital and from general practice.

Drug resistance to 8 different antibiotics in Enterobacteriaceae isolated from different hospitals and two groups of general practitioners was studied. Escherichia coli dominated among the 632 strains investigated. Drug resistance was found in 62% of the 512 hospital strains and in 38% of the 120 strains from general practice. Multiple resistance was common especially in strains from hospital. R factors was found in 23% of the 317 drug-resistant strains from hospital and in 11% of the 46 drug-resistant strains from general practice. Resistance to streptomycin, sulphonamide and tetracycline either alone or in combinations were the most common traits transferred.

Anti-Bacterial Agents↗
PubMed1975

An epidemiologic study of yeasts in elderly denture wearers.

The purpose of this study has been to assess the prevalence of denture stomatitis and candida infection in an elderly Danish population. Ten percent of the population above the age of 65 in a Danish community was selected randomly. The study group consisted of 465 persons wearing a removable maxillary denture, who were examined in their homes. Yeasts were isolated by oral swabs for cultivation and by mucosal and denture scrapings for microscopy. The prevalence of denture stomatitis was 65%. Yeasts, especially C. albicans, were cultivated in most denture wearers with denture stomatitis (Group 1) or with clinically normal palatal mucosa (Group 2). However, large accumulations of hyphae were present in 77% of the patients in Group 1 compared with 47% in Group 2; both hyphae and inflammatory cells were seen in smears in 65% of Group 1 but only in 14% of Group 2. On the other hand, bacterial contamination of the smears was more pronounced in Group 2 than in Group 1. The study has revealed that candida infection and poor denture cleanliness are very common in elderly denture wearers.

Candida↗