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Biomedical subjects

A Skowron-Cendrzak

Publications and source records attributed to A Skowron-Cendrzak.

At least 19 recordsLinked to original sources
PubMed1999

Effect of multiparity on T-cell proliferation response to mitogen stimulation in elderly women.

The purpose of the present study was to investigate whether multiparity in women prevents some age-related changes in their cell function, as is known for age and sex. 49 women from the same village, free of acute inflammation or neoplastic diseases, were selected and divided into the following three groups: nulliparous 1935 years old, nulliparous 65 92 years old and multiparous (3-9 pregnancies) 65-88 years old. The response of their blood-lymphocytes to phytohaemagglutinin-P was estimated by thymidine uptake, number of mitoses and number of sister-chromatid exchanges. The stimulation index, mitotic index and proliferative rate in the aging nulliparous women were significantly lower than in the younger nulliparous women, but the latter group (young nulliparous) did not differ in their lymphocyte parameters assayed, from the values in the aging multiparous women breast-feeding their newborn. It is concluded that when the proliferative capacity to mitogen in elderly multiparous women (after 3-9 pregnancies) is related to breast feeding, it is similar to the proliferative capacity in young women. We suggest therefore, that breast feeding is beneficial not only to the newborns, but also to their mothers.

Adult↗
PubMed1997

Effect of sciatic denervation on cell-mediated immunity.

Local semiallogenic graft-versus-host (GvH) and host-versus-graft (HvG) reactions were induced in mice whose right hind limbs had been partially denervated surgically. The denervation was performed by removing a 2 mm segment of the sciatic nerve, while sham-operated and non-operated mice served as controls. Eight days after denervation the mice were injected with spleen cells from a 3-month-old C57BL female, or from (C57xCBA)F1 male mice, and the GvH and HvG reactions were evaluated, 7 or 10 days later, by quantitatively estimating the weight (enlargement) of the popliteal lymph-nodes. Significant weight increases of 51% (for the GvH reaction) and 63% (for the HvG reaction) were observed in the denervated mice, when compared to the sham-operated ones. This increased cell-type reactivity in a denervated limbs seems to be related to the immunostimulatory activity of cytokines which are released by cells engaged in repair processes of damaged nerves, as well as related to the reduction of the immunosuppressive effect of noradrenergic innervation in the denervated animals.

Adoptive Transfer↗
PubMed1996

The effect of multiparity and lactation periods on the graft versus host reactivity of thymocytes and splenocytes from aging C57BL mice.

The ontogeny of graft versus host (GvH) reactivity of thymocytes and splenocytes was tested in C57BL female mice from the day of birth up to the age of 24 months. In comparison with virgin mice, the effect of a different number of pregnancies, with or without lactation, on the GvH reactivity was examined in middle-aged and aging female mice. The most striking results were obtained with suckling, aging 18-month-old multiparas. After three or more pregnancies with lactation--regardless of the number of pregnancies without suckling--the weight and cell count of the thymus and GvH reactivities on both thymocytes and splenocytes were significantly increased and were comparable with those of middle-aged, 12-month-old virgins. The present results indicate that physiological lactation periods are responsible for the long-lasting, immunoenhancing effect of multiparity, and that the maternal thymus is involved in the mechanism of this phenomenon. A possible role of prolactin rejuvenation of the thymus in suckling multiparas is suggested.

Aging↗
PubMed1995

Age-related changes in graft-versus-host reactivity of spleen cells from male, virgin, or multiparous female Long-Evans rats.

Spleen cell graft-versus-host (GVH) reactivity was determined in male and female, either virgin or breeder, Long-Evans (LE) rats from 3 to 24 months of age. The tests of a regional (popliteal lymph node enlargement index) and a systemic (splenomegaly index, mortality assay) GVH reaction was used. Although the GVH reactivity declined with age in both sexes, the onset of this decline was significantly delayed in 18-month-old virgin females in comparison with 12-month-old males. Moreover, 6 or 7 consecutive pregnancies resulted in significantly enhanced GVH reactivity of 18-24-month-old females. This long-lasting effect of multiparity was observed in females mated either syngeneically or allogeneically. The possible role of neuroendocrine factors in delaying the age-related process of thymic involution in multiparous females is suggested.

Age Factors↗
PubMed1994

Protective effect of lithium on the stress-induced depression of cell-mediated immunity in mice.

The effect of lithium chloride (LiCl) administration on the stress-induced depression of cell-mediated immunity was studied in mice. Two models of stress-induced depression of immunity were used: (1) keeping the animals at a temperature of 4 degrees C twice for 24 h at a 24-h interval, and (2) keeping them in the dark for 96 h. Both kinds of stress significantly decreased the reactivity of cell donors in the graft-versus-host (GVH) reaction and recipients in the host-versus-graft (HVG) reaction. Treatment with LiCl of the cell donors in GVH reaction or the recipients in HVG reaction daily for three weeks before stress application completely abolished the immunosuppressive effect of "cooling" stress, but not "darkness" stress. The LiCl is thus shown as a potential immunomodulator protecting mice from some forms of the stress-induced depression of cell-mediated immunity.

Animals↗
PubMed1994

Immunoreactivity of ageing C57BL female mice rendered tolerant to the H-Y antigen.

Transplantation tolerance to the H-Y antigen, induced in neonatal or young adult C57BL female mice, beneficially affected their immunoreactivity during the process of ageing. The tolerance acquired in 2-month-old females significantly increased their splenocyte graft-versus-host (GVH) reactivity and the production of interleukin-2 (IL-2) at the age of 18 months. The neonatally induced tolerance also resulted in a higher weight of the thymus and a significantly enhanced GVH reactivity of thymocytes in 18-month-old females compared to control mice of the same age.

Aging↗
PubMed1994

Effect of immobilization stress on tumor growth in mice.

The effect of immobilization stress applied daily for 2 h on the development of MF-RSV tumor graft in mice and on survival of the hosts was studied. While a single acute stress applied to mice simultaneously with a transfer of MF-RSV cells stimulated antitumor defense, chronic stress applied both before and/or after tumor cell implantation accelerated the death of recipients. The shortest survival time was found in mice which were stressed for 3 weeks before implantation and on, until their death. Heat shock applied to MF-RSV tumor cells before implantation significantly reduced their growth ability in the hosts.

Animals↗
PubMed1992

The effect of age, sex and breeding on graft versus host reactivity of spleen cells from C57BL mice.

Spleen cell graft versus host (GvH) reactivity was determined in male and female, either virgin or breeder, C57BL mice from 3 to 24 months of age. The GvH reaction was assessed by a local popliteal lymph node assay and by a splenomegaly test for a systemic reaction. Although the GvH reactivity declines progressively with age in both sexes the virgin female response was greater than that of males throughout the period of 6-18 months of age. Two-year-old mice of both sexes were practically unable to mount a GvH reaction. No differences were evident in aging female reactivity after one or two syngeneic pregnancies. On the other hand, 3 or more consecutive pregnancies resulted in enhanced GvH reactivity of 12-24-month-old females, which responded comparably to young virgin mice. This long-lasting immunopotentiating effect of multiparity was similar after 3-4 and 8-9 pregnancies. The possible role of developing foetuses on the maintenance of high GvH reactivity in breeder females is suggested.

Aging↗
PubMed1992

Stress-induced changes in muscarinic and beta-adrenergic binding sites on rat thymocytes and lymphocytes.

This study examined the effect of immobilization stress on the expression of muscarinic and beta-adrenergic receptors on thymocytes and lymphocytes obtained from 5-month-old L-E male rats. After 2 h immobilization (acute stress) there was a significant increase in specific binding of [3H]-DHA to beta-adrenergic receptors on thymocytes and on lymphocytes from the blood but not from the spleen, whereas [3H]-QNB binding to muscarinic receptors in those cells was not altered in comparison with the undisturbed control. Chronic immobilization stress (5 days, for 2 h) decreased the [3H]-QNB binding to lymphocytes collected from the spleen and blood but not from thymus; it caused neither a significant change in the 3H-DHA binding to thymocytes nor lymphocytes obtained from the blood and spleen.

Animals↗
PubMed1992

Down-regulating effect on cellular immunity of the NSF17 factor produced by murine neonatal spleen cells fused with BW 5147 thymoma cells.

The non-specific factor NSF17 produced by mouse newborn splenocytes fused with BW 5147 thymoma cells was tested in vivo for its immunosuppressive activity. NSF17 administered i.p. in a single dose into adult mice significantly decreased the PHA-induced proliferative response of lymphocytes, regional graft-versus-host reaction, and delayed-type hypersensitivity.

Animals↗
PubMed1991

The effect of lithium chloride treatment on cell-mediated immunity in mice.

The immunomodulatory effect of lithium chloride (LiCl) administered i.p. to adult mice (150 mg/kg/day) on cellular immunity in vivo was investigated. A short (6-day) treatment with LiCl of either spleen cell donors in semiallogeneic or xenogeneic GVH reaction, or recipients in semiallogeneic HVG reaction, significantly diminished the early sings of local cell-mediated immunity. A short-term LiCl treatment of donor mice also abrogated systemic GVH reaction in newborn F1 recipients, while a treatment prolonged for 20 days did not alter the high GVH response of spleen cells typical for untreated donors. Thus, a striking time-dependent efficacy of LiCl on the reduction of GVH reaction was found.

Animals↗
PubMed1991

The influence of the age of donors and recipients on the regional graft-versus-host reaction in mice.

Regional graft versus host (GVH) reaction in mice induced by injection of parental strain spleen cells into the footpad of F1, recipients, was measured 7 days later by a popliteal lymph node (PLN) enlargement index. Young, 6-week-old F1 hosts can be used for studying the age- and sex-dependent GVH reactivity of parental strain donors. On the other hand, senescent, 21-24-month-old F1 recipients are able to mount a host versus graft response against parental antigens which may obscure the typical GVH reaction. The PLN enlargement in syngeneic old F1 mice points to an autoimmune mechanism of this phenomenon.

Aging↗
PubMed1990

Ontogenic development of immunoregulation in rats.

Suppressor activity of newborn rat spleen cells is totally inhibited on day 3 after birth by immunoregulatory cells of thymic origin. Contrasuppression represents a necessary, evolutionary step in the ontogenic development of the immune system.

Age Factors↗
PubMed1989

Effect of neonatal spleen and thymus implants on H-Y incompatible skin grafts.

The transplantation reactivity of adult splenectomized C57BL females to H-Y incompatible skin grafts was significantly reduced by the implantation of spleen and thymus from foetal or 0-1-day-old syngeneic female donors. The thymus implant served as a possible source of natural suppressor cell precursors and the spleen implant as a microenvironment for their maturation.

Animals↗
PubMed1988

The role of prostaglandin E in the natural suppressor activity of spleen cells from newborn rats.

Spleen cells from newborn to 2-day-old, but not 3-day-old or older, rats suppress the activity of cells from adult animals in xenogeneic local graft-versus-host (GVH) assay. The duration of neonatal suppressor cell activity can be significantly prolonged by treating newborn rats with exogenous prostaglandin E2 (PGE2), and the suppression was completely abolished by administration of PGE inhibitor indomethacin. The results thus demonstrate an involvement of PGE2 in the mechanism of action of neonatal suppressor cells and may explain some discrepancies concerning the nature of natural suppressor cells present in newborn animals.

Animals↗