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Biomedical subjects

A Skarin

Publications and source records attributed to A Skarin.

45 records · Page 3Linked to original sources
PubMed1983

Ampullary somatostatinoma: psammomatous variant of gastrointestinal carcinoid tumor--an immunohistochemical and ultrastructural study. Report of a case and review of the literature.

A 28-year-old man presented with epigastric pain and obstructive jaundice associated with a histologically and immunologically unusual variant of carcinoid tumor involving the ampulla of Vater. The tumor contained abundant psammoma bodies and exhibited immunoreactivity only for somatostatin. Immunoperoxidase studies for insulin, glucagon, vasoactive intestinal peptide, calcitonin, serotonin, and ACTH had negative results. In contrast to most somatostatinomas of pancreatic origin, clinically this ampullary somatostatinoma was not accompanied by features of the somatostatinoma syndrome. A literature review of the clinical and hormonal features in reported cases of gastrointestinal and pancreatic somatostatinomas is presented.

Adenoma, Islet Cell↗
PubMed1983

Anaerobic comma-shaped bacteria recovered from the human genital tract. A review.

The present survey reviews the current knowledge on comma-shaped anaerobic rods that can be isolated from the human genital tract. It summarizes bacterial characteristics such as morphology, biochemical activities, gas chromatographic patterns and differential diagnostic tests. In particular the existence of two variants of such rods is stressed. These variants differ in length, number of flagella, certain biochemical activities and susceptibility to nitroimidazoles. The occurrence of comma-shaped rods in relation to clinical findings is also discussed.

Bacterial Infections↗
PubMed1983

Antimicrobial susceptibility of comma-shaped bacteria isolated from the vagina.

Comma-shaped bacteria isolated from the posterior vaginal fornix of women with foul-smelling discharge were tested for their antimicrobial susceptibility. The 20 strains studied were tested anaerobically, using agar dilution tests. They were highly susceptible to beta lactam antibiotics, clindamycin, doxycycline, erythromycin, gentamicin and thiamphenicol. On the other hand, the bacteria were relatively resistant to sulfamethoxazole, nalidixic acid and nitroimidazoles. A difference in susceptibility to nitroimidazoles between long and short variants of the comma-shaped bacteria was found; the former being more susceptible than the latter.

Anti-Bacterial Agents↗
PubMed1982

Gas chromatographic study of cellular fatty acids of comma-shaped bacteria isolated from the vagina.

Gas chromatographic analyses were performed of the cellular fatty acids of 16 strains of comma-shaped bacteria isolated from the vagina of women with discharge. Acidic methanolysis of lyophilized bacterial cells was used, followed by splitless injection of hexane extracts onto a fused silica capillary column. Myristic (14:0), hexadecanoic (16:0), octadecadienoic (18:2), octadecenoic (18:1) and octadecanoic (18:0) acids were the major fatty acids found. Long and short variants of the comma-shaped bacteria showed a great similarity in their fatty acid patterns. No hydroxy acids were detected.

Bacteria↗
PubMed1982

Characteristics of anaerobic comma-shaped bacteria recovered from the female genital tract.

Comma-shaped rods isolated from the vagina of women with signs of vaginitis were studied. Three types of rods were found: a long (length about 4 microns), a medium-sized (about 3 microns) and a short (about 1 micron) variant. All three variants had a characteristic cork-screw motility. The long and the medium-sized variants had up to eight flagella, while the short had up to four. The bacteria only grew under anaerobic conditions, although after a great number of passages on artificial culture media the short variant, but not the other variants, could be grown in a microaerophilic atmosphere. In broth media growth of all three variants was stimulated by the addition of 0.3% formatefumarate. On solid media colonies developed after incubation for 48 to 72 hours at 37 degrees C. The long and the medium-sized variants differed from the short variant in that they produced leucine arylamidase, while only the short variant produced alpha-galactosidase. None of the strains utilized carbohydrates; intravariant differences were found in the test results for gelatin and nitrate reduction. All strains of the short variant tested, but none of the long, hydrolysed sodium hippurate. Gas chromatographic analyses showed that all three variants produce acetic, lactic and succinic acids.

Anaerobiosis↗
PubMed1981

Scanning electron microscopic examination of Bacteroides fragilis and Gardnerella vaginalis after exposure to concentration gradients of metronidazole and tinidazole.

Agar diffusion tests with metronidazole and tinidazole were performed with one strain each of Bacteroides fragilis and Gardnerella vaginalis (Haemophilus vaginalis, Corynebacterium vaginalis). Their cell morphology was studied 'in situ' on agar surfaces by means of scanning electron microscopy (SEM). A sharp growth end-point was found for B. fragilis, whereas with G. vaginalis there was a gradual decrease in the number and size of the colonies close to the agar well. The SEM study also revealed elongation of the B. fragilis cells when exposed to high concentrations of either drug. No such elongation of G. vaginalis was observed.

Bacteroides fragilis↗
PubMed1978

Transport of organic anions through the erythrocyte membrane as K+-valinomycin complexes.

K+, Rb+, or Cs+ complexes of valinomycin form ion pair complexes with picric acid and trinitrobenzenesulfonate (TNBS). The formation of a picrate-K+-valinomycin complex is supported by spectral evidence. These complexes have zero net charge and readily permeate the intact erythrocyte membrane. The K+-valinomycin complex has been used to convert the nonpenetrating TNBS into a penetrating covalent probe, making it as useful vectorial probe to measure accessible amino groups of proteins and phospholipids on both sites of the erythrocyte membrane. The enhanced transport of TNBS into the cell by valinomycin is dependent on external K+ in the medium. The entry of TNBS into the cell is manifested by an increased labeling of hemoglobin and membrane phosphatidylethanolamine (PE). Stilbeneisothiocyanatedisulfonate (SITS) and anilinonaphthalenesulfonate (ANS) inhibit both the basal and K+-valinomycin stimulated labeling of PE and hemoglobin by TNBS. The data suggest two independent effects of ANS and SITS, one mediated by an inhibition of the anion transport protein and another by the incorporation of these hydrobic anions into the cell membrane with an increase in negative charge on the membrane which leads to an inhibition of TNBS permeation into the cell by electrostatic repulsion.

Anilino Naphthalenesulfonates↗