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A Siddiqui

Publications and source records attributed to A Siddiqui.

147 records · Page 9Linked to original sources
PubMedDate not supplied

Differential expression of platelet-derived growth factor receptor-beta in an aging model of wound repair.

Impaired wound healing as a result of age is a well-documented phenomenon. However, the overall deficit in healing is substantially increased when the healing wound of an aged animal is ischemic. We hypothesized that both of these deficits are cytokine mediated. We have studied the messenger RNA expression of platelet-derived growth factor receptor-beta, using the rabbit dermal ulcer model of wound repair, in young (3 to 6 months) and aged (48 months and 60 months) rabbits under normal and ischemic conditions. Platelet-derived growth factor receptor-beta mRNA expression was measured with the use of quantitative reverse transcriptase-polymerase chain reaction with incorporation of a synthetic, nonhomologous DNA fragment complementary to platelet-derived growth factor receptor-beta primers as a competitive internal standard. Results in young rabbits showed a large upregulation of platelet-derived growth factor receptor-beta mRNA expression after wounding. In both aged animal groups, platelet-derived growth factor receptor-beta expression was found to be significantly decreased in nonischemic wounds relative to young nonischemic controls. Ischemia was found to have little effect on platelet-derived growth factor receptor-beta mRNA expression in young animals relative to matched controls. However, ischemia induced a large decrease in the platelet-derived growth factor receptor-beta mRNA levels of wounds of aged animals relative to paired aged nonischemic wounds. Results suggest an age-related delay in platelet-derived growth factor receptor-beta mRNA expression in healing wounds, as well as an age-related decline in responsiveness to confounding ischemia.

Journal Article↗
PubMedDate not supplied

Intrathecal fentanyl-induced pruritus is more severe in combination with procaine than with lidocaine or bupivacaine.

BACKGROUND AND OBJECTIVES: Fentanyl is used as an additive to prolong intrathecal anesthesia with both lidocaine and low-dose bupivacaine in the outpatient setting to minimize voiding or discharge delays. Pruritus is the most common side effect. When using procaine as a substitute for lidocaine, we perceived an increased frequency and severity of pruritus. We compared prospectively the frequency and severity of itching with combinations of fentanyl with lidocaine, bupivacaine, and procaine. METHODS: After institutional review board approval, 135 patients requesting neuraxial anesthesia were asked to evaluate the presence and severity (using a 100 point verbal pruritus score [VPS]) of itching 30 minutes after injection of their spinal anesthetic, on arrival to the postanesthesia care unit (PACU), and at the time of resolution of their block. Choice of anesthetic drug and dose and the use of intravenous sedation was left to the discretion of the attending and resident anesthesiologist. RESULTS: Thirty-three patients received lidocaine and fentanyl, 47 received bupivacaine and fentanyl, and 55 received procaine and fentanyl. In the lidocaine group, 21% of patients experienced pruritus compared with 55% of the bupivacaine group and 55% of the procaine group (P =.003). The average VPS at 30 minutes postblock was 18.4 in the procaine group compared with 0 and 5.5 in the lidocaine and bupivacaine groups (P =.06). On admission to the PACU, it was 37 compared with 16 and 20 for lidocaine and bupivacaine, respectively (P =.006). CONCLUSION: Procaine produces a higher frequency of pruritus than that seen with lidocaine-fentanyl combinations and a greater severity of pruritus than seen with lidocaine-fentanyl and bupivacaine-fentanyl spinal anesthesia. Reg Anesth Pain Med 2001;26:252-256.

Adjuvants, Anesthesia↗