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A Shiotani

Publications and source records attributed to A Shiotani.

51 records · Page 3Linked to original sources
PubMed1996

[The transcriptional regulation of the human gastrin gene by EGF].

Epidermal growth factor (EGF) and phorbol esters stimulate the human gastrin promoter through a novel GC-rich DNA element 5'-68GGGGCGGGGTGGGGGG-53 called gERE (gastrin EGF response element). The transcription factor Sp1 and two fast migrating complexes designated gastrin EGF response proteins (gERP1 and gERP2) bind to gERE. Sp1 binds to the 5' half site and gERP bind to the 3' half site. Both the 5' and 3' domains and some overlap between the two domains are required to confer EGF induction. Complex interactions between Sp1 and other factors binding to overlapping gERE half-sites confer EGF responsiveness to the gastrin promoter.

Base Sequence↗
PubMed1995

Epidermal growth factor stimulation of the human gastrin promoter requires Sp1.

Growth factors coordinately regulate a variety of different genes to stimulate cellular proliferation. In the stomach, gastrin, epidermal growth factor (EGF), and transforming growth factor-alpha all mediate gastric mucosal homeostasis by promoting cell renewal. We have previously shown that EGF and phorbol esters stimulate the human gastrin promoter through a novel GC-rich DNA element 5'-(68)GGGGCGGGGTGGGGGG-53 called gERE (gastrin EGF response element). In this report, we show that three factors bind to this element, the transcription factor Sp1 and two fast migrating complexes designated gastrin EGF response proteins (gERP 1 and 2). To understand how these factors bind and confer EGF responsiveness, mutations of gERE were tested in vitro for protein binding and in vivo for promoter activation. Both gel shift assays and UV cross-linking studies revealed that the factors bind to overlapping domains, Sp1 to the 5' half-site and gERP 1 and 2 to the 3' half-site. Placing either the 5' or 3' mutations upstream of a minimal gastrin promoter abolished EGF induction. Therefore both the 5' and 3' domains were required to confer EGF induction. Collectively, these results demonstrate that complex interactions between Sp1 and other factors binding to overlapping gERE half-sites confer EGF responsiveness to the gastrin promoter.

Animals↗
PubMed1995

cAMP regulates gastrin gene expression.

Gastrin is one of the most potent regulators of acid secretion and gastrointestinal cell growth. A variety of signals regulate gastrin release from the antral G cell. However, whether these secretagogues also stimulate gastrin gene expression has not been established. Dramatic increases in gastrin gene expression occur in the stomach after birth and in response to chronic achlorhydria. Moreover, gastrin gene expression in malignant islet cell tumors (gastrinomas) appears to represent reactivation of the fetal pattern of expression in the pancreas. Thus differential expression of the gastrin gene is a reflection of differences in transcriptional control. Since various luminal and humoral factors stimulate the production of cAMP and gastrin secretion in both gastrinoma and antral G cells, we studied the effect of cAMP on gastrin gene expression. Using stable and transient transformants of a pituitary cell line containing the human gastrin gene, we found that cAMP stimulates a three-fold increase in gastrin mRNA levels and that the response maps to elements located between -148 and -40 base pairs upstream from the cap site. Collectively, these studies link an important regulator of gastrin secretion to regulation of gastrin gene expression.

Base Sequence↗
PubMed1994

Surgical treatment for hypopharyngeal cysts with a side-opened direct laryngoscope.

Two cases of hypopharyngeal cyst are reported. Both cysts occurred in the piriform sinus of the hypopharynx. Histopathological examination indicated that both were retention cysts. These cysts were removed by laryngomicrosurgical technique using a side-opened direct laryngoscope. In the cyst with a distinct base, a laryngomicrosurgical snare was used for removal. In the wide-based cyst, the mucous membrane around the cyst was incised with an electrosurgical instrument and then detached to facilitate removal. In this paper, we describe our surgical procedure for removing hypopharyngeal cysts and discuss the causes of such cysts.

Cysts↗
PubMed1994

Primary small cell carcinoma of the larynx.

Small cell carcinoma is a rare tumor of the larynx. We present such a case in a 78-year-old female. The histopathological diagnosis at the time of laryngomicroscopic biopsy was squamous cell carcinoma, upon which basis we initially chose surgical treatment. The surgical specimen, however, revealed small cell carcinoma. Despite the administration of radiotherapy and chemotherapy, the patient died 9 months after initial presentation. We believe that this case illustrates the need for a sufficiently large biopsy specimen in order to arrive at the correct histopathological diagnosis when small cell carcinoma of the larynx is present, and that immunohistochemistry and electron microscopy should be performed to aid the diagnosis.

Aged↗
PubMed1993

Laryngomicrosurgery with carbon dioxide laser for laryngeal papillomatosis: application of a two-stage operation.

Among the many treatments reported, CO2 laser surgery has recently been applied in the field of laryngomicrosurgery to treat laryngeal papilloma. It is exceedingly difficult to cure this disease, however, because of its rapid recurrence following surgery. Incomplete removal of papilloma is believed to be caused by the difficulty of precisely identifying residual papillomas during surgery. In an effort to resolve this problem, we have developed a new surgical method which we call the "two-stage operation," in which a series of two operations are performed about 10 days apart. By the time of the second operation, the bleeding, coagulated blood, and carbonized tissue from the first operation have resolved, and the laryngeal image is much clearer. If additional lesions are found, they are removed at the second operation. We applied this method to several patients with recurrent laryngeal papillomatosis and obtained good results. This new method is clearly useful in the treatment of laryngeal papillomatosis, and it promises to decrease the recurrence rate. In this paper we present the case of a 26-year-old male with laryngeal papilloma who underwent surgery 1 month previously at another hospital. Laryngeal endoscopy revealed multiple papillomas especially of the glottis. The first operation with the CO2 laser was performed on April 16, 1990. Visible lesions appeared to have been completely removed. The second operation was carried out 10 days after the first operation, and the small residual lesions were removed with the CO2 laser. The patient has been disease-free for 18 months.

Adult↗
PubMed1993

Disappearance of metastatic renal cell carcinoma to the base of the tongue after systemic administration of interferon-alpha.

We describe a 41-year-old Japanese man with metastases of the lung, bone, lymph nodes and the base of the tongue 34 months after right radical nephrectomy. One month after systemic administration of human lymphoblastoid interferon (IFN), the metastasis to the base of the tongue completely disappeared, while other metastases increased both in size and number. Though the true effect of IFN-alpha on the metastatic renal cell carcinoma to the base of the tongue is still unknown, our case indicates that the tongue is a possible sensitive site to IFN-alpha therapy.

Adult↗
PubMed1990

Dynamic movement of air tract fluid in lubrication of the larynx during phonation: a basic study using excised canine larynges and experimental air tract fluid by means of X-ray stroboscope system.

Experiments using excised canine larynges and experimental air tract fluid were conducted to clarify the movement of air tract fluid in association with vocal fold vibration by means of X-ray stroboscope system. It was thought that the movement of air tract fluid in lubricating of the larynx during phonation was closely correlated to vocal fold vibration, especially the wave motion of the mucous membrane of the vocal folds and the movement of air tract fluid on the glottis might be one of parameters to evaluate the flexibility of the mucous membrane of the vocal folds.

Animals↗
PubMed1989

Microangiographic study of spinal cord injury and myelopathy.

Post-mortem microangiography has been performed in 12 patients with traumatic cervical cord injury, 2 patients with myelopathy, and one patient with post-traumatic syringomyelia. Microangiography defined 4 zones in the injured spinal cord. In traumatic injury, the size and extent of the non-filling area (Zone 1) was directly related to the injury force. Subsequently these areas became cavitated. Surrounding the non-filling area, widely extending areas demonstrated filling of capillaries but showed damaged neurons and axons (Zone 2). In the chronic stage, Zone 2 replaced by gliomesenchymal tissue. The capillaries in the gliomesenchymal scars revealed a distorted abnormal arrangement (Zone 3). In a case with myelopathy, the hypervascular areas (Zone 4) were observed just around the chronically compressed area. The vascular increases were considered to be a reaction against chronic compression. In a patient with post-traumatic syringomyelia, the vascular network of the posterior grey matter disappeared and the central arteries were distorted by the distending forces of the syrinx. The microangiographs suggested that vascular factors might play a significant role in original cavity formation; but in cavity extension, these factors were not primary.

Adult↗
PubMed1980

Comparative studies on the effects of linoleate and methyl linoleate and their hydroperoxides on the respiration and reactivities of rat heart mitochondria.

Linoleate hydroperoxide and linoleate at concentrations of 100-140 nmol-mg protein activated state 4 respiration of rat heart mitochondria 4.2-fold, increased the apparent enthalpy change of the respiration per gram atom of oxygen consumed from -148 to -226 kJ/O and completely inhibited oxidative phosphorylation. Methyl linoleate hydroperoxide or methyl linoleate did not show the same effects. Further addition of linoleate hydroperoxide or linoleate induced oligomycin-insensitive Mg-ATPase to a level 5 or 2 times, respectively, that obtained with 120 muM dinitrophenol, accompanied by clearing of the mitochondrial suspension and release of malate dehydrogenase from the matrix. Methyl linoleate hydroperoxide had the same effects except that the induced Mg-ATPase activity retained oligomycin sensitivity. Methyl linoleate did not show either effect.

Adenosine Triphosphatases↗
PubMedDate not supplied

Papillary adenocarcinoma possibly arising from an intraductal papilloma of the parotid gland.

We report a case of a 70-year-old female with a papillary adenocarcinoma which we believe to have originated from an intraductal papilloma of the parotid gland. A few cases of intraductal papilloma of a minor salivary gland have been reported; however, to our knowledge, intraductal papilloma and, hence, adenocarcinoma originating from an intraductal papilloma of the parotid gland, have never been described.

Adenocarcinoma, Papillary↗
PubMedDate not supplied

Isolated non-Hodgkin's malignant lymphoma of the larynx presenting as a large pedunculated tumor.

This paper presents a case of non-Hodgkin's lymphoma (NHL) of the larynx. The patient was an 86-year-old man who complained of foreign-body sensation and dysphagia. Laryngoendoscopic examination revealed a large pedunculated mass arising from the left aryepiglottic fold. A diffuse, large, B-cell-type NHL was diagnosed histopathologically. The patient was treated with combination chemotherapy, resulting in complete clinical remission. The clinicopathological features of this case have been described and compared with previously reported cases.

Aged↗