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Biomedical subjects

A Sensi

Publications and source records attributed to A Sensi.

44 records · Page 3Linked to original sources
PubMed1985

A monoclonal antibody (GF 22.1) detecting different patterns of HLA class I cross-reactivities at different dilutions.

A murine cytotoxic monoclonal antibody (GF 22.1) was produced by Balb/c immunization with GRA human lymphoblastoid cells. HLA-A, B and C blanketing and SDS-PAGE analysis of the immunoprecipitate demonstrated MHC class I structures as the targets. Cytotoxic assays were performed with peripheral blood lymphocytes from 84 unrelated donors and from members of 15 families at different antibody dilutions. Statistical analysis was performed by Fisher test on each dilution separately and by Mann-Whitney U test on the dilutions taken all together. The data suggest the detection of a cross-reacting group (B15, A32, B17, B40/w41 and B21) with high affinity and the inclusion of other antigens (B12, B35, A2, B13, A11 and A24) with a lower affinity.

Animals↗
PubMed1984

Impaired HLA capping capacity of peripheral blood lymphocytes in Duchenne muscular dystrophy.

The cap capacity in nine Duchenne muscular dystrophy (DMD) patients and in 23 healthy male subjects (14 adults and nine neonates) has been investigated by inducing capping of HLA molecules. The evaluation of capping percentages ranged in healthy controls from 44 to 61 with a mean value of 53.39 +/- 4.89, while DMD patients displayed cap capacity of percentages varying from 15 to 38 with a mean value of 31.5 +/- 7.42. Statistical significance of the differences between the two groups, assessed by the Mann-Whitney U test, was p less than 0.00003. A correlation was found in DMD patients between cap capacity and age (tau = +0.657, p = 0.012). The results confirm previous reports of Ig capping impairment noted in B cells of the whole lymphocyte population, supporting the hypothesis of a systemic cellular defect in DMD patients. The data obtained suggest that HLA capping could overcome some of the technical difficulties of Ig capping and could be used as a diagnostic aid in antenatal detection of DMD.

Adult↗
PubMed1983

[Possibility of prenatal diagnosis of progressive muscular dystrophy: evaluation of creatine kinase activity in serum and the capping of lymphocytes].

Duchenne muscular dystrophy is a severe inherited disease. The pathogenesis is unknown. Duchenne dystrophy is characterized by a large number of membrane abnormalities, which are manifested by a leakage of muscle enzymes, such as creatine kinase (CK), and a reduction in cap formation in lymphocytes. In the present study serum CK and percentage lymphocyte capping in 8 patients with progressive muscular dystrophy, 6 with different myopathies and normal controls are investigated. Reduced antibody-induced redistribution of membrane antigens of B and T lymphocytes and high serum CK activity is found in boys with Duchenne dystrophy if compared with normal subjects and no correlation exists between the two parameters. Our data indicate that the determination of fetal serum CK activity associated with fetal lymphocyte capping may have diagnostic value in antenatal detection of Duchenne dystrophy.

Adolescent↗
PubMed1983

[Microcytological characteristics of reticulocytes in heterozygous beta-thalassemia].

Mean reticulocyte volume (MRV) and reticulocyte/nonreticulated erythrocyte (R/E) area ratio were determined by a planimetric method in normals and in heterozygous beta-thalassaemia subjects. The MRV in beta-thalassaemia group was significantly less, whereas no difference in the R/E area ratio was observed. These data suggest that in both groups the extent of splenic surface remodeling of the red cells should overlap, whereas the small reticulocyte size of beta-thalassaemia is determined by some alteration of bone marrow function. We suggest that a small MRV in beta-thalassaemia trait could also account for ineffective erythropoiesis, a major bone marrow feature of heterozygous beta-thalassaemia.

Adult↗
PubMedDate not supplied

HLA B51 antigen associated with neutrophil hyper-reactivity.

HLA B51 specificity is strongly associated with Behcet's disease (BD) (for references see Baricordi et al., 1986), a multisystem vasculitis of unknown aetiology, for which an immunological pathogenesis has been proposed (O'Duffy et al., 1983; O'Duffy et al., 1990). Neutrophil abnormalities observed in BD patients even during clinical remission suggest prominent involvement of these phagocytic cells in the pathogenesis of the disease (Niwa et al., 1982). In order to clarify how HLA B51 antigen might confer susceptibility to BD, we have investigated neutrophil function in 13 B51-positive and 13 B51-negative healthy subjects. Lymphocyte spontaneous proliferation and circulating immune complexes were also evaluated. Whereas neutrophils from B51-positive subjects showed an increase in the chemotactic response toward casein (P = 0.003) and LPS (P = 0.033) and also in the PMA-induced superoxide production (P = 0.008) no evidence of enhanced lymphocyte activation emerged. These results suggest that the HLA region can exert a regulatory control on PMN functions.

Adult↗