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Biomedical subjects

A Sen

Publications and source records attributed to A Sen.

At least 253 records · Page 14Linked to original sources
PubMed1978

Production of protein by fungi from agricultural wastes. II. Effect of carbon/nitrogen ratio on the efficiency of substrate utilization and protein production by Rhizoctonia melongina, Pleurotus ostreatus, and Coprinus aratus.

An attempt was made to standardize the C/N ratio at which maximum protein production may be achieved by Rhizoctonia melongina, Pleurotus ostreatus, and Coprinus aratus, using sugarcane bagasse for the former two and wheat straw for the latter in the liquid medium. Three different ways of changing the C/N ratios were tried. The optimum levels of carbon and nitrogen required for the best yield, substrate utilization, and protein production efficiency were worked out.

Agaricales↗
PubMed1978

Production of protein by fungi from agricultural wastes. III. Effect of phosphorus on the efficiency of substrate utilization and protein production by Rhizoctonia melongina, Pleurotus ostreatus, and Coprinus aratus.

An attempt was made to select a source of phosphorus that is readily available and ensures maximum protein efficiency for Rhizoctonia melongina, Pleurotus ostreatus, and Coprinus aratus, using sugarcane bagasse for the former two and wheat straw for the latter one in the liquid medium. Of the six different phosphorus compounds tested, urea phosphate was unanimously preferred by the three fungi. The concentration of urea phosphate for maximum protein output was then standardized.

Agaricales↗
PubMed1978

Production of protein by fungi from agricultural wastes. IV. Effect of certain inorganic salts on the efficiency of substrate utilization and protein production by Rhizoctonia melongina, Pleurotus ostreatus, and Coprinus aratus.

The paper deals with the standardization of the concentrations of magnesium sulphate, zinc sulphate, and ferric chloride in the medium containing sugarcane bagasse (for Rhizoctonia melongina and Pleurotus ostreatus) and wheat straw (for Coprinus aratus) for achieving maximum protein production.

Agaricales↗
PubMed1978

Production of protein by fungi from agricultural wastes. V. Effect of various organic acids and growth promoters on the efficiency of substrate utilization and protein production by Rhizoctonia melongina, Pleurotus ostreatus, and Coprinus aratus.

Results of an investigation made for selecting a source of organic acid and also a growth promoter that can maximize protein production in the three test fungi, viz., Rhizoctonia melongina, Pleurotus ostreatus, and Coprinus aratus from their respective substrates (i.e. sugarcane bagasse for the former two and wheat straw for the latter) are reported. Among organic acids, acetic acid (at 0.1% level) and among the growth promoters ascorbic acid (at 0.1% level) had maximum influence on growth and protein production. Based on the detailed investigations carried out with three test fungi (JAUHRI at. al. 1978, Jauhri and Sen 1978a, b, c, new stuffed media have been formulated for the three test fungi which assure maximum protein efficiency from them.

Acids↗
PubMed1977

Phosphorylation of murine type C viral p12 proteins regulates their extent of binding to the homologous viral RNA.

The purified p12 phosphoprotein of Rauscher murine leukemia virus was fractionated by ion exchange chromatography into subpopulations of molecules containing different amounts of covalently linked phosphate. Of the various phosphorylated forms of p12 protein purified from virions, only a species containing relatively little phosphate can bind in vitro to purified homologous 70S viral RNA. Using ultraviolet irradiation to stabilize ribonucleoprotein complexes in intact virions, the same molecular species of p12 phosphoprotein can be isolated in close association with the 70S viral genome. The results show that phosphorylation of type C viral p12 proteins influences the extent, but not the specificity, of their interaction with homologous viral RNA.

Phosphates↗
PubMed1977

The genome-associated, specific RNA binding proteins of avian and mammalian type C viruses.

A structural protein purified from the Rous sarcoma virus (RSV) can specificially bind in vitro to purified avian, but not mammalian, type C viral RNA. Following ultraviolet irradiation of viral particles under conditions which stabilize the polyploid 70S viral RNA, the same polypeptide can be directly purified from the RSV genome. Based on its electrophoretic mobility in polyacrylamide gels containing sodium dodecylsulfate, the RNA binding protein has been identified as the major phosphoprotein (p19) of avian type C viruses. Similar experiments show that the major phosphoproteins of mammalian type C viruses (p12 for murine viruses and p16 for endogenous primate viruses) are also the specific RNA binding proteins and, similarly, are found closely associated with the 70S RNA genomes in the intact viral particles.

Avian Sarcoma Viruses↗
PubMed1977

Grouping of rhizobial strains--a method based on symbiotic characteristics.

Twenty strains of Rhizobium japonicum and non-inoculated control were used to study seven symbiotic characteristics with two varieties of soybean (Glycine max). The strains were then grouped on the basis of these symbiotic characteristics, using Mahalanobis' D2 statistical method. Eight groups were formed in which two strains stood distinctly aloof, indicating thereby the exceptional nature of these strains over others in their symbiotic behaviour. This method is suggested for selecting exceptional strains for particular symbiotic characteristics as well as for greater N fixing efficiency with varieties and for different agroclimatic conditions.

Rhizobium↗
PubMed1977

Stepwise selection of efficient rhizobial cultures through cultural characteristics.

Nodulation and shoot nitrogen of two varieties of soybean (Glycine max) were studied with twenty strains of Rhizobium japonicum. A number of cultural characteristics of the strains in isolation to the symbiotic system were also studied. A stepwise selection method was employed for detecting efficient cultures through the cultural characteristics which showed association with the steps in the symbiotic system. Nodulation of one variety was found to be associated with the dehydrogenase activity and the growth of microbes in the medium containing soil extract, whereas the nodulation of another variety showed association with the growth in the media containing asparagine and tryptophane. The shoot nitrogen of one nodulated cultivar correlated with the microbial growth in Elkan's medium in the medium containing serine and glucose, whereas the shoot nitrogen of the other nodulating variety correlated with the growth of the cultures in the medium containing aspartic acid. The validity of this approach to the problem for detecting efficient strains through cultural characteristics was discussed.

India↗
PubMed1976

Specific binding of the type C viral core protein p12 with purified viral RNA.

The major viral phosphoproteins (p12) of the Rauscher murine leukemia virus (R-MuLV) and the simian sarcoma-associated virus (SSAV) bind in vitro to their homologous 70S and 35S viral RNAs. Using purified 32P-labeled RNA and 125I-labeled p12 protein, complexes that are stabilized by formaldehyde-cross-linking can be readily detected after velocity gradient centrifugation. The in vitro reconstructed ribonucleoprotein complexes are seen only with p12 proteins incubated with viral RNAs isolated from the same type C viruses; no such complexes form with heterologous protein-RNA mixtures. Homologous but not heterologous p12 molecules compete with radiolabeled p12 protein for the specific viral RNA binding sites. The competition assay permits the detection of 10 ng of viral p12 protein. The major internal protein of type C viruses (p30) does not bind to viral RNA using identical assay conditions. From the specific activities of the radiolabeled components and also by equilibrium sedimentation analysis, we estimate that fewer than 15 molecules of p12 protein bind to each molecule of viral RNA. Both the specificity and stoichiometry of the p12-RNA interactions suggest that these RNA tumor virus proteins have a regulatory role in cells.

Binding Sites↗